Skjoldryggen terminal moraine on the mid-Norwegian shelf

Terminal moraines are relatively large ridges of diamictic glacial debris produced at the outermost margins of past glaciers and ice sheets. Their identification on land is important in mapping the maximum extent of Quaternary ice sheets (Svendsen et al. 2004). In marine environments where fast-flowing ice streams reach the shelf edge during full-glacial intervals, moraine ridges are not usually present and the seafloor is characterised by parallel-to-flow streamlined sediments (Ottesen & Dowdeswell 2009). Submarine terminal moraine ridges, by contrast, appear more typical of slower-flowing ice margins (Dahlgren et al. 2002; Dowdeswell & Elverhøi 2002).This is the author accepted manuscript. The final version is available from the Geological Society of London via https://doi.org/10.1144/M46.6

3D seismic imagery of mega-scale glacial lineations and flow-switching by ice streams on the Norwegian continental shelf

This is the author accepted manuscript. The final version is available from the Geological Society of London via https://doi.org/doi.org/10.1144/M46.96Streamlined glacial landforms, produced by deformation of soft sediments at glacier beds (Dowdeswell et al. 2004; Ó Cofaigh et al. 2005; King et al. 2009), provide clear evidence of the direction of ice-flow at the time of their formation (e.g. Ottesen et al., 2005; Livingstone et al. 2012). Where sets of streamlined landforms are present at or close to the seafloor, swath-bathymetric imagery enables the reconstruction of multiple phases of ice-flow (e.g. Greenwood et al. 2012). In many cases, however, key morphological evidence is buried on palaeo-shelves within the Quaternary glacial sedimentary record (Fig. 1c-d), and can only be analysed by tracing these buried horizons in 3D-seismic datasets (Dowdeswell et al. 2007)

Molecular Targets of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) within the Zebrafish Ovary: Insights into TCDD-induced Endocrine Disruption and Reproductive Toxicity

TCDD is a reproductive toxicant and endocrine disruptor, yet the mechanisms by which it causes these reproductive alterations are not fully understood. In order to provide additional insight into the molecular mechanisms that underlie TCDD\u27s reproductive toxicity, we assessed TCDD-induced transcriptional changes in the ovary as they relate to previously described impacts on serum estradiol concentrations and altered follicular development in zebrafish. In silico computational approaches were used to correlate candidate regulatory motifs with observed changes in gene expression. Our data suggest that TCDD inhibits follicle maturation via attenuated gonadotropin responsiveness and/or depressed estradiol biosynthesis, and that interference of estrogen-regulated signal transduction may also contribute to TCDD\u27s impacts on follicular development. TCDD may also alter ovarian function by disrupting various signaling pathways such as glucose and lipid metabolism, and regulation of transcription. Furthermore, events downstream from initial TCDD molecular-targets likely contribute to ovarian toxicity following chronic exposure to TCDD. Data presented here provide further insight into the mechanisms by which TCDD disrupts follicular development and reproduction in fish, and can be used to formulate new hypotheses regarding previously documented ovarian toxicity

Landforms characteristic of inter-ice stream settings on the Norwegian and Svalbard continental margins

This is the author accepted manuscript. The final version is available from the Geological Society of London via https://doi.org/10.1144/M46.16

Fan-like sediments on outer Haltenbanken, mid-Norwegian shelf

Subaqueous fans formed in marine and lacustrine settings have been reported from Quaternary and more ancient glacier-influenced environments (e.g. Powell 1990; Dowdeswell et al. 2015). Their presence is often taken to imply a palaeo-glacial environment abundant in sediment-rich meltwater, whose load was sourced from glacial erosion and transported in subglacial channels to the ice margin. The sedimentology of these fans sometimes exhibits bedforms indicative of high-energy water-flow, suggesting that rapid drain

Galvanic vestibular stimulation produces cross-modal improvements in visual thresholds

Background: Stochastic resonance (SR) refers to a faint signal being enhanced with the addition of white noise. Previous studies have found that vestibular perceptual thresholds are lowered with noisy galvanic vestibular stimulation (i.e., "in-channel" SR). Auditory white noise has been shown to improve tactile and visual thresholds, suggesting "cross-modal" SR. Objective: We aimed to study the cross-modal impact of noisy galvanic vestibular stimulation (nGVS) (n=9 subjects) on visual and auditory thresholds. Methods: We measured auditory and visual perceptual thresholds of human subjects across a swath of different nGVS levels in order to determine if a subject-specific best nGVS level elicited a reduction in thresholds as compared the no noise condition (sham). Results: We found an 18% improvement in visual thresholds (p = 0.026). Among the 7 of 9 subjects with reduced thresholds, the average improvement was 26%. Subjects with higher (worse) visual thresholds with no stimulation (sham) improved more than those with lower thresholds (p = 0.005). Auditory thresholds were unchanged by vestibular stimulation. Conclusions: These results are the first demonstration of cross-modal improvement with nGVS, indicating galvanic vestibular white noise can produce cross-modal improvements in some sensory channels, but not all.Comment: 15 pages, 7 figure

Heat-shock responsive genes identified and validated in Atlantic cod (Gadus morhua) liver, head kidney and skeletal muscle using genomic techniques

Background: Daily and seasonal changes in temperature are challenges that fish within aquaculture settings cannot completely avoid, and are known to elicit complex organismal and cellular stress responses. We conducted a large-scale gene discovery and transcript expression study in order to better understand the genes that are potentially involved in the physiological and cellular aspects of stress caused by heat-shock. We used suppression subtractive hybridization (SSH) cDNA library construction and characterization to identify transcripts that were dysregulated by heat-shock in liver, skeletal muscle and head kidney of Atlantic cod. These tissues were selected due to their roles in metabolic regulation, locomotion and growth, and immune function, respectively. Fish were exposed for 3 hours to an 8°C elevation in temperature, and then allowed to recover for 24 hours at the original temperature (i.e. 10°C). Tissue samples obtained before heat-shock (BHS), at the cessation of heat-shock (CS), and 3, 12, and 24 hours after the cessation of heat-shock (ACS), were used for reciprocal SSH library construction and quantitative reverse transcription - polymerase chain reaction (QPCR) analysis of gene expression using samples from a group that was transferred but not heat-shocked (CT) as controls.Results: We sequenced and characterized 4394 ESTs (1524 from liver, 1451 from head kidney and 1419 from skeletal muscle) from three "forward subtracted" libraries (enriched for genes up-regulated by heat-shock) and 1586 from the liver "reverse subtracted" library (enriched for genes down-regulated by heat-shock), for a total of 5980 ESTs. Several cDNAs encoding putative chaperones belonging to the heat-shock protein (HSP) family were found in these libraries, and "protein folding" was among the gene ontology (GO) terms with the highest proportion in the libraries. QPCR analysis of HSP90α and HSP70-1 (synonym: HSPA1A) mRNA expression showed significant up-regulation in all three tissues studied. These transcripts were more than 100-fold up-regulated in liver following heat-shock. We also identified HSP47, GRP78 and GRP94-like transcripts, which were significantly up-regulated in all 3 tissues studied. Toll-like receptor 22 (TLR22) transcript, found in the liver reverse SSH library, was shown by QPCR to be significantly down-regulated in the head kidney after heat-shock.Conclusion: Chaperones are an important part of the cellular response to stress, and genes identified in this work may play important roles in resistance to thermal-stress. Moreover, the transcript for one key immune response gene (TLR22) was down-regulated by heat-shock, and this down-regulation may be a component of heat-induced immunosuppression

Transcriptomic Responses of Atlantic Salmon (Salmo salar) to Environmental Enrichment during Juvenile Rearing

Captive rearing programs (hatcheries) are often used in conservation and management efforts for at-risk salmonid fish populations. However, hatcheries typically rear juveniles in environments that contrast starkly with natural conditions, which may lead to phenotypic and/or genetic changes that adversely affect the performance of juveniles upon their release to the wild. Environmental enrichment has been proposed as a mechanism to improve the efficacy of population restoration efforts from captive-rearing programs; in this study, we examine the influence of environmental enrichment during embryo and yolk-sac larval rearing on the transcriptome of Atlantic salmon (Salmo salar). Full siblings were reared in either a hatchery environment devoid of structure or an environment enriched with gravel substrate. At the end of endogenous feeding by juveniles, we examined patterns of gene transcript abundance in head tissues using the cGRASP-designed Agilent 4×44K microarray. Significance analysis of microarrays (SAM) indicated that 808 genes were differentially transcribed between the rearing environments and a total of 184 gene ontological (GO) terms were over- or under-represented in this gene list, several associated with mitosis/cell cycle and muscle and heart development. There were also pronounced differences among families in the degree of transcriptional response to rearing environment enrichment, suggesting that gene-by-environment effects, possibly related to parental origin, could influence the efficacy of enrichment interventions

Fish and chips: Various methodologies demonstrate utility of a 16,006-gene salmonid microarray

BACKGROUND: We have developed and fabricated a salmonid microarray containing cDNAs representing 16,006 genes. The genes spotted on the array have been stringently selected from Atlantic salmon and rainbow trout expressed sequence tag (EST) databases. The EST databases presently contain over 300,000 sequences from over 175 salmonid cDNA libraries derived from a wide variety of tissues and different developmental stages. In order to evaluate the utility of the microarray, a number of hybridization techniques and screening methods have been developed and tested. RESULTS: We have analyzed and evaluated the utility of a microarray containing 16,006 (16K) salmonid cDNAs in a variety of potential experimental settings. We quantified the amount of transcriptome binding that occurred in cross-species, organ complexity and intraspecific variation hybridization studies. We also developed a methodology to rapidly identify and confirm the contents of a bacterial artificial chromosome (BAC) library containing Atlantic salmon genomic DNA. CONCLUSION: We validate and demonstrate the usefulness of the 16K microarray over a wide range of teleosts, even for transcriptome targets from species distantly related to salmonids. We show the potential of the use of the microarray in a variety of experimental settings through hybridization studies that examine the binding of targets derived from different organs and tissues. Intraspecific variation in transcriptome expression is evaluated and discussed. Finally, BAC hybridizations are demonstrated as a rapid and accurate means to identify gene content

Development and Experimental Validation of a 20K Atlantic Cod (Gadus morhua) Oligonucleotide Microarray Based on a Collection of over 150,000 ESTs

The collapse of Atlantic cod (Gadus morhua) wild populations strongly impacted the Atlantic cod fishery and led to the development of cod aquaculture. In order to improve aquaculture and broodstock quality, we need to gain knowledge of genes and pathways involved in Atlantic cod responses to pathogens and other stressors. The Atlantic Cod Genomics and Broodstock Development Project has generated over 150,000 expressed sequence tags from 42 cDNA libraries representing various tissues, developmental stages, and stimuli. We used this resource to develop an Atlantic cod oligonucleotide microarray containing 20,000 unique probes. Selection of sequences from the full range of cDNA libraries enables application of the microarray for a broad spectrum of Atlantic cod functional genomics studies. We included sequences that were highly abundant in suppression subtractive hybridization (SSH) libraries, which were enriched for transcripts responsive to pathogens or other stressors. These sequences represent genes that potentially play an important role in stress and/or immune responses, making the microarray particularly useful for studies of Atlantic cod gene expression responses to immune stimuli and other stressors. To demonstrate its value, we used the microarray to analyze the Atlantic cod spleen response to stimulation with formalin-killed, atypical Aeromonas salmonicida, resulting in a gene expression profile that indicates a strong innate immune response. These results were further validated by quantitative PCR analysis and comparison to results from previous analysis of an SSH library. This study shows that the Atlantic cod 20K oligonucleotide microarray is a valuable new tool for Atlantic cod functional genomics research