Generating mice with targeted mutations.

Journal ArticleMutational analysis is one of the most informative approaches available for the study of complex biological processes. It has been particularly successful in the analysis of the biology of bacteria, yeast, the nematode worm Caenorhabditis elegans and the fruit fly Drosophila melanogaster. Extension of this approach to the mouse, through informative, was far less successful relative to what has been achieved with these simpler model organisms. This is because it is not numerically practical in mice to use random mutagenesis to isolate mutations that affect a specified biological process of interest. Nonetheless, biological phenomena such as a sophisticated immune response, cancer, vascular disease or higher-order cognitive function, to mention just a few, must analyzed in organisms that show such phenomena, and for this reason geneticists and other researchers have turned to the mouse. Gene targeting, the means for creating mice with designed mutations in almost any gene, was developed as an alternative to the impractical use of random mutgenesis for pursing genetic analysis in the mouse. Now gene targeting has advanced the genomic manipulations possible in mice to a level that can be matched only in far simple organisms such as bacteria and yeast

Predicting the establishment of hair follicle patterns in the developing mouse

Symposium in Honor of Patrick Tam FRS "60 Years & Still Gastrulating"Conference Theme: From Embryology to Disease Mechanism

Understanding the molecular impact of Sox9Y440X in campomelic dysplasia

Symposium in Honor of Patrick Tam FRS "60 Years & Still Gastrulating"Conference Theme: From Embryology to Disease Mechanism

Sox2 dosage defines the specification and survival of sensory neurons in the inner ear

Symposium in Honor of Patrick Tam FRS "60 Years & Still Gastrulating"Conference Theme: From Embryology to Disease Mechanism

Predicting the spatiotemporal dynamics of hair follicle patterns in the developing mouse

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Impact of Sox2 dosage on sensory neurogenesis and prosensory versus non-sensory cell fate in the inner ear

Session: Cell Fate, Potential and DifferentiationConference Theme: Insights into Gene Networks, Disease Models and Evolutionary Mechanisms of Neural Crest and Cranial Placode Developmen

A mouse model for hearing and balance defects in campomelic dysplasia

The 6th Molecular Biology of Hearing and Deafness Conference, Hinxton, U.K., 11-14 July 2007