583 research outputs found
Turning Counseling Students into Researchers: Enhancing Quantitative Research Courses with an Experiential Learning Activity
Research methods and application are crucial aspects of most counseling practitioners and scholars’ lives, yet practical experience with development and implementation of research projects is usually limited to doctoral level dissertations. This article describes an experiential research project that has been integrated into counseling research methods courses at both the master’s level and the doctoral level. In this mentored research activity, students move through the entire research process in one semester. They begin with a notion and finish with a submission for publication. Based on student responses, implementing this process in a research methodology course is recommended
Assisting High School Students with Career Indecision Using a Shortened Form of the Career Construction Interview
A shortened form of the Career Construction Interview (CCI) was used to help high school students struggling with the career decision making process. The shortened instrument is described, as well as, its use with eleventh grade high school students who had low levels of career concern and career curiosity. Students who completed the exercise reported several themes that are introduced and discussed in the article. These themes reflected that the intervention was helpful and facilitated student self understanding and career exploration. Practical applications for school counselors are discussed
Counselor Supervision in Jamaica: An Initial Exploration
The context and conceptualization of counselor supervision in Jamaica was explored using a mixed methods design. A majority of practicum supervisors (81%) from each of the four master’s level counseling programs in the country participated in the study. Descriptive survey data and individual interviews were used to explore current practices and gain perspective on future needs of the profession. Results indicate that the primary form of supervision provided is case consultation with groups of students and the supervisors indicate a need for more supervision training. Suggestions for training and development in supervision and direction for further research are discussed
Human Services Students Preferences for Master\u27s Level Training
Human Services students close to graduation are seeking employment in the field, but many are also considering their future career paths and the training needed to reach their long-term career goals. Knowing if bachelor\u27s level students desire graduate degrees, which focus they prefer, and how they would like to pursue the degrees may contribute to the decision-making of educators, employers, and students. This exploratory study, therefore, examined human services students\u27 preferences for master\u27s level training. Students\u27 responses reflected preferences for several types of master\u27s programs, direct acceptance, and online delivery. These themes and their implications for educators, employers, and students are discussed
Quantitative Footprinting Analysis. Binding to a Single Site
The theory for measuring ligand binding constants from footprinting autoradiographic data associated with a single binding site is derived. If the ligand and DNA cleavage agent compete for a common site, the spot intensities are not proportional to the amount of DNA not blocked by ligand. The analysis of a single site is experimentally illustrated by using results for the anticancer drug actinomycin D interacting with the duplex d(TAGCGCTA)2 as probed with the hydrolytic enzyme DNase I
Students\u27 Experiences with Different Course Delivery Modalities: On Campus, Online, and Satellite
In an effort to adapt to the technological advances of this century, the training of human services professionals has grown from traditional classrooms and satellite programs to online education. Many human services programs are under pressure from their universities and students to expand into online education. This study examined 252 students’ experiences and perceptions of their Bachelors of Science program as it transitioned to offering courses online in addition to on campus and satellite sites. Students’ narrative responses reflected 4 themes: convenience, interactions, learning preference, and technology. These themes and their implications for educators and students are discussed
Clarifying the Identity of Human Services Through a Content Analysis of Programmatic Accreditation
Throughout the United States, accrediting bodies serve as voluntary self-regulating entities designed to ensure accountability and quality assurance at the institutions that seek accreditation. To examine the impact of accreditation on the field of human services, a mixed-method content analysis was utilized. The 50 human services programs accredited by the Council for Standards on Human Services Education (CSHSE) as of July of 2018 were examined. Researchers also employed a triangulated approach to understand these programs through an analysis of Carnegie Classifications, regional accreditation agencies, and institutions programmatic websites. Results offer insight into how the CSHSE influences the professional identity of human services thorough: (1) variations in the length of time programs have been accredited; (2) regional distinctions between accredited programs; (3) and the titles of programs accredited by the CSHSE
Site-Specific Binding Constants for Actinomycin D on DNA Determined from Footprinting Studies
We report site-specific binding constants for the intercalating anticancer drug actinomycin D (Act-D), binding to a 139-base-pair restriction fragment from pBR 322 DNA. The binding constants are derived from analysis of footprinting experiments, in which the radiolabeled 139-mer is cleaved using DNase I, the cleavage products undergo gel electrophoresis, and, from the gel autoradiogram, spot intensities, proportional to amounts of cleaved fragments, are measured. A bound drug prevents DNase I from cleaving at -7 bonds, leading to decreased amounts of corresponding fragments. With the radiolabel on the 3’ end of the noncoding strand (A-label), we measured relative amounts of 54 cleavage products at 25 Act-D concentrations. For cleavage of the 139-mer with the label on the 3’ end of the coding strand (G-label), relative amounts of 43 cleavage products at 11 Act-D concentrations were measured. These measurements give information about - 120 base pairs of the restriction fragment (- 12 turns of the DNA helix); in this region, 14 strong and weak Act-D binding sites were identified. The model used to interpret the footprinting plots is derived in detail. Binding constants for 14 sites on the fragment are obtained simultaneously. It is important to take into account the effect of drug binding at its various sites on the local concentration of probe elsewhere. It is also necessary to include in the model weak as well as strong Act-D sites on the carrier DNA which is present, since the carrier DNA controls the free-drug concentration. As expected, the strongest sites are those with the sequence (all sequences are 5’ - 3’) GC, with TGCT having the highest binding constant, 6.4 X lo6 M-l. Sites having the sequence GC preceded by G are weak binding sites, having binding constants approximately 1 order of magnitude lower than those of the strong sites. Also, the non-GC-containing sequences CCG and CCC bind Act-D with a binding constant comparable to those of the weak GGC sites. The analysis may reveal drug-induced structural changes on the DNA, which are discussed in terms of the mechanism of Act-D binding
Designing Program Evaluation Outcomes to Mirror Council for Human Services Education (CSHSE) Standards at the Baccalaureate Degree Level
The article highlights the designing program evaluation and its maintenance with the Council for Human Services Education (CSHSE) standards at the baccalaureate degree level in universities & colleges. Topics include that CSHSE requires all accredited human services educational programs to articulate strategies for improving their programs and its importance in the accreditation process
Determination of Netropsin-DNA Binding Constants from Footprinting Data
A theory for deriving drug-DNA site binding constants from footprinting data is presented. Plots of oligonucleotide concentration, as a function of drug concentration, for various cutting positions on DNA are required. It is assumed that the rate of cleavage at each nucleotide position is proportional to the concentration of enzyme at that nucleotide and to the probability that the nucleotide is not blocked by drug. The probability of a nucleotide position not being blocked is calculated by assuming a conventional binding equilibrium for each binding site with exclusions for overlapping sites. The theory has been used to evaluate individual site binding constants for the antiviral agent netropsin toward a 139 base pair restriction fragment of pBR-322 DNA. Drug binding constants, evaluated from footprinting data in the presence of calf thymus DNA and poly(dGdC) as carrier and in the absence of carrier DNA, were determined by obtaining the best fit between calculated and experimental footprinting data. Although the strong sites on the fragment were all of the type (T-A),, the value of the binding constant was strongly sequence dependent. Sites containing the dinucleotide sequence 5\u27-TA-3\u27 were found to have significantly lower binding constants than those without this sequence, suggesting that an adenine-adenine clash produces a DNA structural alteration in the minor groove which discourages netropsin binding to DNA. The errors, scope, and limitations associated with the method are presented and discussed
- …