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Not AvailableBreeding experiments were conducted in Heteropneustes fossilis employing GnRH-based inducing agents like ovaprim, ovatide and wova-FH. The catfish responded well to ovaprim (0.40-0.55 ml/kg), ovatide (0.45-0.55 mg/kg) and wova-FH (0.50-0.60 mg/kg). Ovaprim gave better results in terms of fertilization and hatching success on low dose and latency period was also less as compared to ovatide and wova-FH. The overall performance of ovaprim was better when compared with the other two drugs. Among ovatide and wova-FH, the latter appeared to be better in spawning performance. The gonadotrophs (cynophils), distributed in the proximal pars distalis (PPD) of the pituitary gland, exhibited hyperactivity and degranulation in ovaprim, ovatide and wova-FH induced-bred H. fossilis suggesting release of the hormone (gonadotropin) in response to administration of the drugs.Not Availabl

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Not AvailableCatla catla is widely distributed across India, Pakistan,Bangladesh, Nepal and Myanmar (Reddy, 2005) and animportant species in fisheries and aquaculture (Chonder,1999). In India, C. catla is naturally found in the Indo-Gangetic river system. A drastic population decline in C. catlaby about 87% in the middle stretches of the Ganges in theperiod 1958–1994 (Payne et al., 2004) was mainly due tooverfishing and habitat alterations (Reddy, 2005). Addition-ally, farmed Catla could also have escaped, intermingling withwild stocks and leading to the loss of genetic variation(Ferguson et al., 1995).Indian Council of AgriculturalResearch, New Delh

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Not AvailableTwo commercially important mussel species are recorded from the Indian coast: green mussel Perna viridis (Linnaeus, 1758) and brown mussel P. indica (Kuriakose and Nair, 1976). Apart from this, a third type referred to as parrot mussel, which has shell shape of brown mussel, but with green shell colouration and suspected to be the hybrid of the above two species has also been reported from Kollam coast of Kerala, where both the species co-occur. In the present work, genetic identity of parrot and sympatric mussel species was determined using protein and genomic DNA markers. Protein markers viz. Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and allozymes and the genomic DNA marker Random Amplified Polymorphic DNA (RAPD) were used for determining genetic identity of the three mussel groups. The green and brown mussels could be clearly differentiated using SDS PAGE. The parrot mussel protein pattern was similar to that of brown mussel, except for an additional band of molecular weight 48.7 Kda which is unique to brown mussel. Genus specific protein bands for Perna viz. 66 Kda, 43 Kda and 14.3 Kda, were detected in this study. Allozyme electrophoresis also followed a similar pattern. Of the 10 allozyme loci studied, seven revealed speciesspecific diagnostic differences between P.viridis and P.indica. They were AAT-1* (Aspartate Amino Transferase-1*), AAT-2*, ME (Malic Enzyme)*, PGM-2*(Phospo Gluco Mutase-2*), EST-1* (Esterase- 1*), EST-2*, IcDH* (Isocitrate Dehydrogenase)*. Parrot mussel shared all the alleles of brown mussel, and no hybrid pattern was observed. Species-specific alleles clearly differentiated green mussel from both brown and parrot mussel. The genetic distance of green mussel from brown mussel, estimated from allozyme data was 1.1145 and with parrot mussel it was 1.105. The genetic distance between parrot mussel and brown mussel was negligibly low (0.0005). Using allozyme and RAPD data, the Nei’s Unbiased Measures of genetic distance were calculated and the dendograms prepared based on these values clearly depicted the separation of parrot mussel from green mussel as well as the close resemblance of parrot mussel with brown mussel. The higher gene flow (1.1539) determined using RAPD marker also hints that brown and parrot mussel may be acting as single interbreeding population. Hence this study using molecular tools to test the genetic identity of parrot mussel has helped to conclude that parrot mussel is only a morphotype of brown mussel and not a true hybrid of the two.Not Availabl

The third international hackathon for applying insights into large-scale genomic composition to use cases in a wide range of organisms.

In October 2021, 59 scientists from 14 countries and 13 U.S. states collaborated virtually in the Third Annual Baylor College of Medicine & DNANexus Structural Variation hackathon. The goal of the hackathon was to advance research on structural variants (SVs) by prototyping and iterating on open-source software. This led to nine hackathon projects focused on diverse genomics research interests, including various SV discovery and genotyping methods, SV sequence reconstruction, and clinically relevant structural variation, including SARS-CoV-2 variants. Repositories for the projects that participated in the hackathon are available at https://github.com/collaborativebioinformatics