Processing of mono-, di- and tricistronic transfer RNAs precursors in a spinach or pea chloroplast soluble extract

International audienc

Location of the genes for cytochrome f, subunit IV of the b6/f complex, the α-subunit of CF1 ATP-synthase and subunit III of the CFα ATP-synthase on the barley chloroplast genome

The genes for cytochrome f (petA), subunit IV of the cytochrome b6/f complex (petI), the α-subunit of CF1-ATP-synthase (atpA) and subunit III of CFα-ATP-synthase (atpH) have been mapped by heterologous hybridisation of wheat probes to the barley chloroplast genome

Import of Polypeptides into Chloroplasts

The majority of the protein components of chloroplasts are synthesized outside the organelle and are subsequently imported. These imported polypeptides are produced as precursors containing an amino-terminal extension. Recent experiments have demonstrated that foreign polypeptides can be imported into chloroplasts when fused to these amino-terminal extensions. This ability provides exciting opportunities for improvement of economically important plants through genetic manipulation