Expression of Neurog1 Instead of Atoh1 Can Partially Rescue Organ of Corti Cell Survival

In the mammalian inner ear neurosensory cell fate depends on three closely related transcription factors, Atoh1 for hair cells and Neurog1 and Neurod1 for neurons. We have previously shown that neuronal cell fate can be altered towards hair cell fate by eliminating Neurod1 mediated repression of Atoh1 expression in neurons. To test whether a similar plasticity is present in hair cell fate commitment, we have generated a knockin (KI) mouse line (Atoh1KINeurog1) in which Atoh1 is replaced by Neurog1. Expression of Neurog1 under Atoh1 promoter control alters the cellular gene expression pattern, differentiation and survival of hair cell precursors in both heterozygous (Atoh1+/KINeurog1) and homozygous (Atoh1KINeurog1/KINeurog1) KI mice. Homozygous KI mice develop patches of organ of Corti precursor cells that express Neurog1, Neurod1, several prosensory genes and neurotrophins. In addition, these patches of cells receive afferent and efferent processes. Some cells among these patches form multiple microvilli but no stereocilia. Importantly, Neurog1 expressing mutants differ from Atoh1 null mutants, as they have intermittent formation of organ of Corti-like patches, opposed to a complete ‘flat epithelium’ in the absence of Atoh1. In heterozygous KI mice co-expression of Atoh1 and Neurog1 results in change in fate and patterning of some hair cells and supporting cells in addition to the abnormal hair cell polarity in the later stages of development. This differs from haploinsufficiency of Atoh1 (Pax2cre; Atoh1f/+), indicating the effect of Neurog1 expression in developing hair cells. Our data suggest that Atoh1KINeurog1 can provide some degree of functional support for survival of organ of Corti cells. In contrast to the previously demonstrated fate plasticity of neurons to differentiate as hair cells, hair cell precursors can be maintained for a limited time by Neurog1 but do not transdifferentiate as neurons

Local Public Services Costs and the Geography of Development: Evidence from Florida Counties

Theory suggests that the spatial distribution of development within a local jurisdiction affects the costs of providing local public services. We use GINI coefficients to characterize these distributions at the county level and estimate the effects on real per capita expenditures from reductions in the spatial concentration of all buildings and nine alternative types of development. We also estimate the effect on expenditures from expansions in the developed area of a county. The results obtained from a panel of Florida counties confirm our theory and suggest that the geography of development within a county affects public services costs

The Millage Rate Offset and Property Tax Revenue Stability

One of the alleged virtues of the property tax is that it produces stable revenues regardless of price movements in real estate markets. One explanation for this is that local governments adjust their millage rates to offset changes in their property tax base. Little evidence, however, exists on the strength of this millage rate offset mechanism. We hypothesize that the importance of this mechanism will vary among local governments depending upon the monopoly power that they possess. The results provide strong support for our hypothesis

The Millage Rate Offset and Property Tax Revenue Stability

One of the alleged virtues of the property tax is that it produces stable revenues regardless of price movements in real estate markets. One explanation for this is that local governments adjust their millage rates to offset changes in their property tax base. Little evidence, however, exists on the strength of this millage rate offset mechanism. We hypothesize that the importance of this mechanism will vary among local governments depending upon the monopoly power that they possess. The results provide strong support for our hypothesis

The Fiscal Impacts of Alternative Land Uses

Current knowledge of the fiscal impacts of alternative land uses comes largely from cost of community services (CCS) case studies, the results of which are viewed skeptically in the literature due to methodological concerns. To address these issues, we develop an econometric approach that allows us to capture both the direct and indirect relationships between a complete accounting of community fiscal measures and the full distribution of acres of land uses in a jurisdiction. Using a novel panel data set, we extensively document empirical correlations that have not yet been formally established in the literature. Our results are inconsistent with the broad conclusions of CCS studies: neither a shift from agricultural to residential land nor a shift to commercial land is associated with a significant change in the budget. We provide support for and insights into our results by extending our analysis to finer revenue/expenditure and land-use subcategorie

The Fiscal Impacts of Alternative Land Uses

Current knowledge of the fiscal impacts of alternative land uses comes largely from cost of community services (CCS) case studies, the results of which are viewed skeptically in the literature due to methodological concerns. To address these issues, we develop an econometric approach that allows us to capture both the direct and indirect relationships between a complete accounting of community fiscal measures and the full distribution of acres of land uses in a jurisdiction. Using a novel panel data set, we extensively document empirical correlations that have not yet been formally established in the literature. Our results are inconsistent with the broad conclusions of CCS studies: neither a shift from agricultural to residential land nor a shift to commercial land is associated with a significant change in the budget. We provide support for and insights into our results by extending our analysis to finer revenue/expenditure and land-use subcategorie