Chaetognaths and oceanography on Georges Bank

A dense breeding population of chaetognaths, Sagitta elegans, was observed on northeastern Georges Bank during autumn, 1978. Four grid surveys conducted on the northeast region of the Bank over the period 17-30 October 1978 revealed that the high concentration patch of adult S. elegans. about 56 km on long axis, remained essentially on the eastern part of the grid in weakly stratified water of 67 m average depth. Current meter measurements indicated residual flow to the southeast sufficiently high to transport a passively drifting organism out of the area over the two weeks of study. Discrete vertical sampling showed that the reproductively mature adults underwent extensive migration from surface to near bottom by day in response to the solar cycle. The smaller immature stages did not migrate vertically or only had limited range; they were found throughout the water column, both day and night, and were spread across the shallow well-mixed as well as deeper weakly stratified waters. Using current meter records, transport of the migrating adults and essentially nonmigrating immature stages of the S. elegans population at different sites within the study area was modeled. The simulations revealed that the migration of adults to near bottom for several hours of the day, where the current was assumed to approach zero, resulted in a longer residence time on the Bank than for nonmigrating immatures. More importantly, it is shown how the strong lunar tidal regime characteristic of Georges Bank may be an important mechanism for generating smaller scale variability on the order of \u3c50 km and \u3c2 weeks

Health Policy Development: Health Promotion and Illness Prevention Among Older Adults in Illinois

This paper offers recommendations for health promotion and illness prevention for older adults in Illinois and offers a structure for policy development. It is based on the premise that policy development should consider the experiences of older adults, family members, and health care providers delivering direct services. Personal experiences and expert analyses were examined in the context of strategies to promote health. This example of policy development is discussed in terms of its application to alternative methods of social and health change and identifies roles for the sociological practitioner

Distribution of Short-finned Squid (IIlex illecebrosus) Larvae and Juveniles in Relation to the Gulf Stream Frontal Zone Between Florida and Cape Hatteras

Catches of rhynchoteuthion type C' larvae, which are considered to be II/ex illecebrosus, and I. il/ecebrosus juveniles over the edge of the continental shelf between Cape Hatteras and Florida are examined in relation to the water masses and their dynamics along the Gulf Stream~Slope Water frontal zone. All larvae and juveniles were captured at stations where the temperature~salinity (T-S) properties of the upper 50 m of the water column were closely grouped, with temperatures of 21.0° to 23.5° C and salinities of 36.30 to 36.80, which are very similar to those of Continental Edge Water. Although the actual depths of capture of the larvae and juveniles in the bongo and midwater trawl tows are unknown, it is likely that the majority were taken in the upper 50 m. Larvae and juveniles were intermixed along the entire frontal zone, but there are indications of some differences in microscale distribution. Although juveniles were nearly always captured at stations where larvae were taken, larvae were captured at only 44% of the stations where juveniles were found. The intermixture of larvae and juveniles with a broad range of size (mantle length) indicates that spawning occurs either along the Gulf Stream~Slope Water frontal zone south of Cape Hatteras or in a relatively small area to the south of the surveyed area. The possible role of frontal eddies in causing the intermixture of larvae and juveniles is discussed

Dive, food and exercise effects on blood microparticles in Steller sea lions (Eumetopias jubatus) : exploring a biomarker for decompression sickness

Author Posting. © The Author(s), 2016. This is the author's version of the work. It is posted here by permission of American Physiological Society for personal use, not for redistribution. The definitive version was published in American Journal of Physiology - Regulatory, Integrative and Comparative Physiology 310 (2016): R596-R601, doi:10.1152/ajpregu.00512.2015.Recent studies of stranded marine mammals indicate that exposure to underwater military sonar may induce pathophysiological responses consistent with decompression sickness (DCS). However, DCS has been difficult to diagnose in marine mammals. We investigated whether blood microparticles (MPs, measured as number/μl plasma), which increase in response to decompression stress in terrestrial mammals, are a suitable biomarker for DCS in marine mammals. We obtained blood samples from trained Steller sea lions (Eumetopias jubatus, 4 adult females) wearing time-depth recorders that dove to predetermined depths (either 5 or 50 m). We hypothesized that MPs would be positively related to decompression stress (depth and duration underwater). We also tested the effect of feeding and exercise in isolation on MPs using the same blood sampling protocol. We found that feeding and exercise had no effect on blood MP levels, but that diving caused MPs to increase. However, blood MP levels did not correlate with diving depth, relative time underwater, and presumably decompression stress―possibly indicating acclimation following repeated exposure to depth.Funding for this project was provided by the Office of Naval Research to MM (ONR Award # N00014-12-10388) and SRT (ONR Award # N00014-13-10614). Additional support was provided by the National Oceanic and Atmospheric Administration through the North Pacific Marine Science Foundation and the North Pacific Universities Marine Mammal Research Consortium.2017-02-0

Serum microRNA profiling to distinguish papillary thyroid cancer from benign thyroid masses

Objectives: Papillary thyroid cancer (PTC) is increasing in incidence. Fine needle aspiration is the gold standard for diagnosis, but results can be indeterminate. Identifying tissue and serum biomarkers, like microRNA, is therefore desirable. We sought to identify miRNA that is differentially expressed in the serum of patients with PTC. Methods: Serum miRNA was quantified in 31 female thyroidectomy patients: 13 with benign disease and 18 with PTC. qPCR results were compared for significant fold-changes in 175 miRNAs, against a pooled control. Results: 128 miRNA qualified for analysis. There were identifiable fold-changes in miRNA levels between benign and control, and between PTC and control. There were statistically significant fold changes in the level of four miRNAs between benign and PTC: hsa-miR-146a-5p and hsa-miR-199b-3p were down-regulated, while hsa-let7b-5p and hsa-miR-10a-5p were up-regulated. Conclusions: MicroRNA is differentially expressed in the serum of patients with PTC. Serum miRNA has the potential to aid in thyroid cancer diagnosis

Trade-offs between foraging efficiency and pup feeding rate of lactating northern fur seals in a declining population

Foraging strategies and their resulting efficiency (energy gain to cost ratio) affect animals’ survival and reproductive success and can be linked to population dynamics. However, they have rarely been studied quantitatively in free-ranging animals. We investigated foraging strategies and efficiencies of wild northern fur seals Callorhinus ursinus during their breeding season to understand potential links to the observed population decline in the Bering Sea. We equipped 20 lactating females with biologgers to determine at-sea foraging behaviours. We measured energy expenditure while foraging using the doubly-labelled water method, and energy gained using (1) the types and energy densities of prey consumed, and (2) the number of prey capture attempts (from acceleration data). Our results show that seals employed 2 foraging strategies: one group (40%) fed mostly in oceanic waters on small, high energy-density prey, while the other (60%) stayed over the shallow continental shelf feeding mostly on larger, lower quality fish. Females foraging in oceanic waters captured 3 times more prey, and had double the foraging efficiencies of females that foraged on-shelf in neritic waters. However, neritic seals made comparatively shorter trips, and likely fed their pups ~20 to 25% more frequently. The presence of these strategies which either favor foraging efficiency (energy) or frequency of nursing (time) might be maintained in the population because they have similar net fitness outcomes. However, neither strategy appears to simultaneously maximize time and energy allocated to nursing, with potential impacts on the survival of pups during their first year at sea

Serum microRNA profiling to distinguish papillary thyroid cancer from benign thyroid masses

Objectives: Papillary thyroid cancer (PTC) is increasing in incidence. Fine needle aspiration is the gold standard for diagnosis, but results can be indeterminate. Identifying tissue and serum biomarkers, like microRNA, is therefore desirable. We sought to identify miRNA that is differentially expressed in the serum of patients with PTC. Methods: Serum miRNA was quantified in 31 female thyroidectomy patients: 13 with benign disease and 18 with PTC. qPCR results were compared for significant fold-changes in 175 miRNAs, against a pooled control. Results: 128 miRNA qualified for analysis. There were identifiable fold-changes in miRNA levels between benign and control, and between PTC and control. There were statistically significant fold changes in the level of four miRNAs between benign and PTC: hsa-miR-146a-5p and hsa-miR-199b-3p were down-regulated, while hsa-let7b-5p and hsa-miR-10a-5p were up-regulated. Conclusions: MicroRNA is differentially expressed in the serum of patients with PTC. Serum miRNA has the potential to aid in thyroid cancer diagnosis

The Hippo component YAP localizes in the nucleus of human papilloma virus positive oropharyngeal squamous cell carcinoma

Background: HPV infection causes cervical cancer, mediated in part by the degradation of Scribble via the HPV E6 oncoprotein. Recently, Scribble has been shown to be an important regulator of the Hippo signaling cascade. Deregulation of the Hippo pathway induces an abnormal cellular transformation, epithelial to mesenchymal transition, which promotes oncogenic progression. Given the recent rise in oropharyngeal HPV squamous cell carcinoma we sought to determine if Hippo signaling components are implicated in oropharyngeal squamous cell carcinoma. Methods: Molecular and cellular techniques including immunoprecipiations, Western blotting and immunocytochemistry were used to identify the key Hippo pathway effector Yes-Associated Protein (YAP)1. Oropharyngeal tissue was collected from CO2 laser resections, and probed with YAP1 antibody in tumor and pre-malignant regions of HPV positive OPSCC tissue. Results: This study reveals that the Scribble binding protein Nitric Oxide Synthase 1 Adaptor Protein (NOS1AP) forms a complex with YAP. Further, the NOS1APa and NOS1APc isoforms show differential association with activated and non-activated YAP, and impact cellular proliferation. Consistent with deregulated Hippo signaling in OPSCC HPV tumors, we see a delocalization of Scribble and increased nuclear accumulation of YAP1 in an HPV-positive OPSCC. Conclusion: Our preliminary data indicates that NOS1AP isoforms differentially associate with YAP1, which, together with our previous findings, predicts that loss of YAP1 enhances cellular transformation. Moreover, YAP1 is highly accumulated in the nucleus of HPV-positive OPSCC, implying that Hippo signaling and possibly NOS1AP expression are de-regulated in OPSCC. Further studies will help determine if NOS1AP isoforms, Scribble and Hippo components will be useful biomarkers in OPSCC tumor biology