37 research outputs found
Probing the plateau-insulator quantum phase transition in the quantum Hall regime
We report quantum Hall experiments on the plateau-insulator transition in a
low mobility In_{.53} Ga_{.47} As/InP heterostructure. The data for the
longitudinal resistance \rho_{xx} follow an exponential law and we extract a
critical exponent \kappa= .55 \pm .05 which is slightly different from the
established value \kappa = .42 \pm .04 for the plateau transitions. Upon
correction for inhomogeneity effects, which cause the critical conductance
\sigma_{xx}^* to depend marginally on temperature, our data indicate that the
plateau-plateau and plateau- insulator transitions are in the same universality
class.Comment: 4 pages, 4 figures (.eps
Sn delta-doping in GaAs
We have prepared a number of GaAs structures delta-doped by Sn using the
well-known molecular beam epitaxy growth technique. The samples obtained for a
wide range of Sn doping densities were characterised by magnetotransport
experiments at low temperatures and in high magnetic fields up to 38 T.
Hall-effect and Shubnikov-de Haas measurements show that the electron densities
reached are higher than for other delta-dopants, like Si and Be. The maximum
carrier density determined by the Hall effect equals 8.4x10^13 cm^-2. For all
samples several Shubnikov-de Haas frequencies were observed, indicating the
population of multiple subbands. The depopulation fields of the subbands were
determined by measuring the magnetoresistance with the magnetic field in the
plane of the delta-layer. The experimental results are in good agreement with
selfconsistent bandstructure calculations. These calculation shows that in the
sample with the highest electron density also the conduction band at the L
point is populated.Comment: 11 pages text (ps), 9 figures (ps), submitted to Semicon. Science
Tech
The key role of smooth impurity potential in formation of hole spectrum for p-Ge/Ge_{1-x}Si_x heterostructures in the quantum Hall regime
We have measured the temperature (0.1 <= T <= 15 K) and magnetic field (0 <=
B <= 12 T) dependences of longitudinal and Hall resistivities for the
p-Ge_0.93Si_0.07/Ge multilayers with different Ge layer widths 10 <= d_w <= 38
nm and hole densities p_s = (1-5)10^11 cm^-2. Two models for the long-range
random impurity potential (the model with randomly distributed charged centers
located outside the conducting layer and the model of the system with a spacer)
are used for evaluation of the impurity potential fluctuation characteristics:
the random potential amplitude, nonlinear screening length in vicinity of
integer filling factors nu = 1 and nu = 2 and the background density of state
(DOS). The described models are suitable for explanation of the unusually high
value of DOS at nu = 1 and nu = 2, in contrast to the short-range impurity
potential models. For half-integer filling factors the linear temperature
dependence of the effective QHE plateau-to-plateau transition width nu_0(T) is
observed in contrast to scaling behavior for systems with short-range disorder.
The finite T -> 0 width of QHE transitions may be due to an effective low
temperature screening of smooth random potential owing to Coulomb repulsion of
electrons.Comment: Accepted for publication in Nanotechnolog
Removal of Heterologous Sequences from Plasmodium falciparum Mutants Using FLPe-Recombinase
Genetically-modified mutants are now indispensable Plasmodium gene-function reagents, which are also being pursued as genetically attenuated parasite vaccines. Currently, the generation of transgenic malaria-parasites requires the use of drug-resistance markers. Here we present the development of an FRT/FLP-recombinase system that enables the generation of transgenic parasites free of resistance genes. We demonstrate in the human malaria parasite, P. falciparum, the complete and efficient removal of the introduced resistance gene. We targeted two neighbouring genes, p52 and p36, using a construct that has a selectable marker cassette flanked by FRT-sequences. This permitted the subsequent removal of the selectable marker cassette by transient transfection of a plasmid that expressed a 37Β°C thermostable and enhanced FLP-recombinase. This method of removing heterologous DNA sequences from the genome opens up new possibilities in Plasmodium research to sequentially target multiple genes and for using genetically-modified parasites as live, attenuated malaria vaccines
Phase Diagram of Integer Quantum Hall Effect
The phase diagram of integer quantum Hall effect is numerically determined in
the tight-binding model, which can account for overall features of recently
obtained experimental phase diagram. In particular, the quantum Hall plateaus
are terminated by two distinct insulating phases, characterized by the Hall
resistance with classic and quantized values, respectively, which is also in
good agreement with experiments.Comment: 4 pages, RevTex, 4 PostScript figures; one new figure is added; minor
modifications in the tex
Integer quantum Hall transition in the presence of a long-range-correlated quenched disorder
We theoretically study the effect of long-ranged inhomogeneities on the
critical properties of the integer quantum Hall transition. For this purpose we
employ the real-space renormalization-group (RG) approach to the network model
of the transition. We start by testing the accuracy of the RG approach in the
absence of inhomogeneities, and infer the correlation length exponent nu=2.39
from a broad conductance distribution. We then incorporate macroscopic
inhomogeneities into the RG procedure. Inhomogeneities are modeled by a smooth
random potential with a correlator which falls off with distance as a power
law, r^{-alpha}. Similar to the classical percolation, we observe an
enhancement of nu with decreasing alpha. Although the attainable system sizes
are large, they do not allow one to unambiguously identify a cusp in the
nu(alpha) dependence at alpha_c=2/nu, as might be expected from the extended
Harris criterion. We argue that the fundamental obstacle for the numerical
detection of a cusp in the quantum percolation is the implicit randomness in
the Aharonov-Bohm phases of the wave functions. This randomness emulates the
presence of a short-range disorder alongside the smooth potential.Comment: 10 pages including 6 figures, revised version as accepted for
publication in PR
Plasmodium Protease ROM1 Is Important for Proper Formation of the Parasitophorous Vacuole
Apicomplexans are obligate intracellular parasites that invade host cells by an active process leading to the formation of a non-fusogenic parasitophorous vacuole (PV) where the parasite replicates within the host cell. The rhomboid family of proteases cleaves substrates within their transmembrane domains and has been implicated in the invasion process. Although its exact function is unknown, Plasmodium ROM1 is hypothesized to play a role during invasion based on its microneme localization and its ability to cleave essential invasion adhesins. Using the rodent malaria model, Plasmodium yoelii, we carried out detailed quantitative analysis of pyrom1 deficient parasites during the Plasmodium lifecycle. Pyrom1(-) parasites are attenuated during erythrocytic and hepatic stages but progress normally through the mosquito vector with normal counts of oocyst and salivary gland sporozoites. Pyrom1 steady state mRNA levels are upregulated 20-fold in salivary gland sporozoites compared to blood stages. We show that pyrom1(-) sporozoites are capable of gliding motility and traversing host cells normally. Wildtype and pyrom1(-) sporozoites do not differ in the rate of entry into Hepa1β6 hepatocytes. Within the first twelve hours of hepatic development, however, only 50% pyrom1(-) parasites have developed into exoerythrocytic forms. Immunofluorescence microscopy using the PVM marker UIS4 and transmission electron microscopy reveal that the PV of a significant fraction of pyrom1(-) parasites are morphologically aberrant shortly after invasion. We propose a novel function for PyROM1 as a protease that promotes proper PV modification to allow parasite development and replication in a suitable environment within the mammalian host
Human monoclonal antibodies targeting carbonic anhydrase IX for the molecular imaging of hypoxic regions in solid tumours
BACKGROUND: Hypoxia, which is commonly observed in areas of primary tumours and of metastases, influences response to treatment. However, its characterisation has so far mainly been restricted to the ex vivo analysis of tumour sections using monoclonal antibodies specific to carbonic anhydrase IX (CA IX) or by pimonidazole staining, after the intravenous administration of this 2-nitroimidazole compound in experimental animal models.METHODS: In this study, we describe the generation of high-affinity human monoclonal antibodies (A3 and CC7) specific to human CA IX, using phage technology.RESULTS: These antibodies were able to stain CA IX ex vivo and to target the cognate antigen in vivo. In one of the two animal models of colorectal cancer studied (LS174T), CA IX imaging closely matched pimonidazole staining, with a preferential staining of tumour areas characterised by little vascularity and low perfusion. In contrast, in a second animal model (SW1222), distinct staining patterns were observed for pimonidazole and CA IX targeting. We observed a complementary pattern of tumour regions targeted in vivo by the clinical-stage vascular-targeting antibody L19 and the anti-CA IX antibody A3, indicating that a homogenous pattern of in vivo tumour targeting could be achieved by a combination of the two antibodies.CONCLUSION: The new human anti-CA IX antibodies are expected to be non-immunogenic in patients with cancer and may serve as broadly applicable reagents for the non-invasive imaging of hypoxia and for pharmacodelivery applications. British Journal of Cancer (2009) 101, 645-657. doi: 10.1038/sj.bjc.6605200 www.bjcancer.com Published online 21 July 2009 (C) 2009 Cancer Research U