16 research outputs found
Durability of SVR in chronic hepatitis C patients treated with peginterferon-a2a/ribavirin in combination with a direct-acting anti-viral
SUMMARY Background The introduction of direct-acting anti-virals has increased sustained virological response (SVR) rates in chronic hepatitis C genotype 1 infection. At present, data on long-term durability of viral eradication after successful triple therapy are lacking
Torque teno (TTV) viral load as a biomarker of immunosuppressive strength after kidney transplantation in children
Micro RNAs miR-106a, miR-122 and mir-197 are increased in severe acute viral hepatitis with coagulopathy
P09: ATTRITION RATES IN MULTIPLE MYELOMA UNDER REAL WORLD CONDITIONS – AN ANALYSIS FROM THE AUSTRIAN MYELOMA REGISTRY
Comparative Analysis of Cleistanthus collinus Aqueous Leaf Extract and Fractions for its Antibacterial Potential
Comparative evaluation of molecular methods for the quantitative measure of torquetenovirus (TTV) viremia, the new surrogate marker of immune competence
BACKGROUND:
Torquetenovirus (TTV) viremia is emerging as a promising tool to assess functional immune competence, to predict post-transplant immune-related complications, and eventually to customize immunosuppression.
METHODS:
In this study, 327 blood samples were tested using two real-time PCR (rtPCR) assays both targeted to the untranslated region of TTV genome. The first assay was an in-house rtPCR developed by our group, the second one was the recently marketed TTV R-GENE\uae assay.
RESULTS:
In the validation study, the TTV R-GENE\uae showed good performances in precision and reproducibility, and a sensitivity as low as 12 TTV DNA copies/ml, like previously reported for the in-house rtPCR. Bland-Altman analysis showed that the mean difference between the two methods was -0.3 Log copies/ml. In the comparison study, 69% and 72% of samples were detected positive by rtPCR and TTV R-GENE\uae, respectively (94% concordance, \u3ba = 0.88). Performances did not differ between the two rtPCRs by type of TTV group examined. When a newly-developed in-house digital droplet PCR was applied for TTV quantification and used as alternative method of comparison on 94 samples, the results strongly correlated with those obtained by the two rtPCR methods (99% concordance).
CONCLUSION:
In summary, all the molecular methods assayed are highly sensitive and accurate in quantitation of TTV DNA in blood samples