53 research outputs found
Oligonucleotide hybridization monitored by surface plasmon enhanced fluorescence spectroscopy with bio-conjugated core/shell quantum dots. Influence of luminescence blinking
No full textSurface plasmon resonance spectroscopy and quartz crystal microbalance study of streptavidin film structure effects on biotinylated DNA assembly and target DNA hybridization
No full text
Surface plasmon resonance spectroscopy and quartz crystal microbalance study of MutS binding with single Thymine-Guanine mismatched DNA
No full textDetection of Point Mutation and Insertion Mutations in DNA Using a Quartz Crystal Microbalance and MutS, a Mismatch Binding Protein
No full text10.1021/ac035175gAnalytical Chemistry762489-494ANCH
Surface plasmon resonance spectroscopy and quartz crystal microbalance study of streptavidin film structure effects on biotinylated DNA assembly and target DNA hybridization
No full textReal-time label-free monitoring of the cellular response to osmotic stress using conventional and long-range surface plasmons
No full textSingle molecule imaging of odorant receptors in supported planar lipid membranes
No full textOdorant receptors are an excellent example of natural superiority in binding specific small and hydrophobic molecules. Difficulties in expression, isolation and solubilisation of these receptors have been overcome recently by in vitro synthesis and incorporation of receptors into a planar lipid membrane [1,2]. The incorporation density and the mobility of the membrane embedded receptors were analyzed at the single-molecule level by means of image correlation spectroscopy [3,4]. Instead of a confocal scanning microscope for the image acquisition, we used our total internal reflection fluorescence microscope and an ultra-sensitive CCD camera. The non-uniform excitation intensity requested a modification of the image correlation analysis, i.e. a normalization of the image intensity with the excitation intensity distribution
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