PENGARUH KONSENTRASI HOMOGENAT JAMUR Pythium Aphanidermatum (Edson) Fitzp terhadap Kandungan Ajmalisin dalam Kultur Agregat Sel Catharanthus Roseus (L.) G. Don

An experiment on the effect of elicitor concentrations derived from Pythium aphanidermatum (Edson) Fitzp on ajmalicine content of Catharanthus roseus (L.) G. Don Cell aggregates culture has been conducted. The following concentrations of elicitor tested were 0.05; 0.5; 1.0 and 5.0 mg DW/mL. The harvesting times were 0; 18; 36 and 72 hours after elicitation. The ajmalicine was analyzed qualitatively and quantitavely by using High Performance Liquid Chromatography (HPLC). Ajmalicine content was influenced by the concentration of elicitor and harvesting time. The highest content of ajmalicine in the cell aggregates (13.089 ± 0.086 µg/gDW) was achieved by addition 1.0 mg DW/mL of elicitor after 18 hours whilts in the medium (346.728 ± 2.843 µg/gDW) was achieved by addition 1.0 mg DW/mL of elicitor after 36 hour

Rasio Seks Jernang (Daemonorops Draco (Willd.)blume) Pada Populasi Alami Dan Budidaya: Implikasi Untuk Produksi Biji

Jernang (Daemonorops draco (Willd.) Blume) is a dioecious rattan species that produces resin on female individuals. The sex of jernang can only be identified from its inflorescences morphology. Sex survey was conducted in 5 populations of jernang, 3 populations of which in the Bukit Tigapuluh National Park (BTNP), 1 population in the secondary forest in Jambi province and 1 population in the area of rubber plantation in Jambi province. Chi-Squared test on the sex ratio of jernang showed that the ratio of the male to female in their natural habitat (Bengayoan and Tebo, 2 parts of the BTNP ecosystem and the Sepintun secondary forest) was distorted from the normal ratio of 1:1, while the cultivated jernang in Nunusan population (BTNPT) and Mandiangin were not significantly different from the normal sex ratio of 1:1. Female individuals from the cultivated population in the Mandiangin (planted in 1997) has an average number of stems per clump more on individual males (27.12) than females (26.92). The result of this study indicated that the D. draco has a good reproductive strategy and thus suitable for cultivation

Methods for environmental monitoring of DOE waste disposal and storage sites. Semiannual progress report, November 1, 1985--March 31, 1986

This progress report contains an account of recent research efforts carried out at the Oak Ridge Research Institute, to raise antibodies in New Zealand White Rabbits which are specific for various strains of Thiobacillus ferrooxidans bacteria. It is then intended to use the antibodies, in a subsequent phase of the project, to develop one or more ELISAs, which would be used to analyze, both in the laboratory and in the field, the numbers and extent of dispersion of these microorganisms at acid mine drainage sites. This is important because it is the metabolic consequences of unrestricted dissemination of these bacteria which are responsible in large measure for the environmentally damaging acid run-off. Efforts to control the proliferation of these microorganisms have been relatively unfocused up to the present, because of the lack of suitably discriminating methods of assaying the effects of treatment. In this report, the authors describe the work carried out in the first section of the project, which has been concerned with the raising of the antibodies to the Thiobacillus ferrooxidans cultures. They given an account of how the various cultures were grown, how they were treated before being injected into rabbits, the injection protocol, how the animals were bled, and how the formation of IgG and its degree of specificity was assessed. Finally, they describe how large quantities of the various IgG fractions were prepared, and how and where they were stored