Analysis of the antibiotic resistance profiles in methicillin-sensitive s. Aureus pathotypes isolated on a commercial rabbit farm in Italy

The breeding of meat rabbits is an important sector in the livestock industry in Italy. The focus of this study was to describe the antibiotic resistance profile distribution among the Methicillin-sensitive Staphylococcus aureus isolated in a rabbit farm. From 400 animals of different ages and three farm workers, 96 randomly selected strains isolated from various anatomical sites and lesions were analysed. According to spa typing and the resistance profiles towards veterinary and human antibiotics, 26 pathotypes were identified. The highest resistance was observed against Tetracyclines (92.3%) and Macrolides (80.8%), while almost all were susceptible to Penicillins, according to the limited use of β-lactams on the farm. In total, 92.3% of pathotypes were multidrug resistant (MDRs). Two MDR pathotypes belonging to the t2802 spa type were isolated from both farmers and rabbits. Age categories harboured significantly different pathotypes (p = 0.019), while no association was found between pathotypes and lesions (p = 0.128) or sampling sites (p = 0.491). The antibiotic resistance was observed to increase with the time spent in the farm environment (age category). The selective pressure exerted by antibiotic use acted by giving advantage to more resistant strains rather than by lowering susceptibility to various drug categories within strains

Holocentric Chromosomes of Luzula elegans Are Characterized by a Longitudinal Centromere Groove, Chromosome Bending, and a Terminal Nucleolus Organizer Region

The structure of holocentric chromosomes was analyzed in mitotic cells of Luzula elegans. Light and scanning electron microscopy observations provided evidence for the existence of a longitudinal groove along each sister chromatid. The centromere-specific histone H3 variant, CENH3, colocalized with this groove and with microtubule attachment sites. The terminal chromosomal regions were CENH3-negative. During metaphase to anaphase transition, L. elegans chromosomes typically curved to a sickle-like shape, a process that is likely to be influenced by the pulling forces of microtubules along the holocentric axis towards the corresponding microtubule organizing regions. A single pair of 45S rDNA sites, situated distal to Arabidopsis-telomere repeats, was observed at the terminal region of one chromosome pair. We suggest that the 45S rDNA position in distal centromere-free regions could be required to ensure chromosome stability. Copyright (C) 2011 S. Karger AG, Base

CSF biochemical correlates of mixed affective states

To evaluate the question of whether “mixed” bipolar disorder is a distinct entity, we compared selected cerebrospinal fluid (CSF) biochemical parameters from patients with bipolar disorder, mixed, to those with mania and major depression. Fourteen patients in each category (DSM-III) were studied with regard to CSF HVA, 5HIAA, sodium, potassium, calcium, and magnesium levels under carefully controlled conditions. CSF HVA, 5HIAA, and sodium were found to be significantly higher in manics than in major depressives. Discriminant analysis of the biochemical variables of the mixed affective group identified two biochemically distinct and clinically different subgroups of seven patients each, one resembling the manic group and the other the major depressive group. These findings suggest that mixed affective states do not exist as a separate entity, but are compsed of two subgroups obtained from the manic and major depressive categories.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/66203/1/j.1600-0447.1988.tb06339.x.pd

Emplacement of a felsic dyke swarm during progressive heterogeneous deformation, Eastern Elba Dyke Complex (Island of Elba, Italy)

Magmatic and sub-solidus fabrics in intrusive rocks are frequently used to infer the relative timing of deformation with respect to magma emplacement and cooling. Here, we describe the relationships between strain and fabric development in leucogranite sheets (pegmatite, aplite) emplaced into shear zones that localized post-thermal peak deformation in the contact aureole of an upper crustal pluton (<0.2 GPa) on the Island of Elba, Italy. The leucogranite sheets present igneous, mylonitic, and cataclastic fabrics. Detailed meso- and microscopic structural analysis suggests that the dykes emplaced in the shear zones behaved as competent, rigid bodies during mylonitic deformation of the host rocks. Thermal modelling indicates that emplacement and cooling of the sheets occurred very rapidly (a few days to years) compared to typical tectonic strain rates and strain accumulation timescales in the host rocks. Such a fast cooling does not allow melt or magma-induced thermal softening in the host rocks during deformation. The development of mylonitic and cataclastic fabrics in the dykes was controlled by the localized activation of fluid-controlled reaction softening mechanisms (mylonitic fabric) and embrittlement during cooling in sites of high-strain (cataclastic fabric). We show that a broad spectrum of fabrics can form in igneous sheet intrusions emplaced at the same time and crustal level. The coexistence of isotropic (non-foliated igneous) versus anisotropic (mylonitic and cataclastic) fabrics in igneous sheet intrusions should therefore be evaluated in terms of tectonic strain rates, cooling rates, thermal state of the host, distribution of heterogeneous strain, and activation of strain softening mechanisms. Our observations highlight that the concepts of pre-, syn-, late- and post-tectonic fabrics in intrusive igneous rocks should be used with caution when interpreting relative timing relationships between deformation and magmatism