77 research outputs found
Recovery of state-specific potential of molecular motor from single-molecule trajectory
We have developed a novel method to evaluate the potential profile of a
molecular motor at each chemical state from only the probe's trajectory and
applied it to a rotary molecular motor F-ATPase. By using this method, we
could also obtain the information regarding the mechanochemical coupling and
energetics. We demonstrate that the position-dependent transition of the
chemical states is the key feature for the highly efficient free-energy
transduction by F-ATPase.Comment: 5 pages, 5 figure
Investigations of the pi N total cross sections at high energies using new FESR: log nu or (log nu)^2
We propose to use rich informations on pi p total cross sections below N= 10
GeV in addition to high-energy data in order to discriminate whether these
cross sections increase like log nu or (log nu)^2 at high energies, since it is
difficult to discriminate between asymptotic log nu and (log nu)^2 fits from
high-energy data alone. A finite-energy sum rule (FESR) which is derived in the
spirit of the P' sum rule as well as the n=1 moment FESR have been required to
constrain the high-energy parameters. We then searched for the best fit of pi p
total cross sections above 70 GeV in terms of high-energy parameters
constrained by these two FESR. We can show from this analysis that the (log
nu)^2 behaviours is preferred to the log nu behaviours.Comment: to be published in Phys. Rev. D 5 pages, 2 eps figure
Spectral Duality in Integrable Systems from AGT Conjecture
We describe relationships between integrable systems with N degrees of
freedom arising from the AGT conjecture. Namely, we prove the equivalence
(spectral duality) between the N-cite Heisenberg spin chain and a reduced gl(N)
Gaudin model both at classical and quantum level. The former one appears on the
gauge theory side of the AGT relation in the Nekrasov-Shatashvili (and further
the Seiberg-Witten) limit while the latter one is natural on the CFT side. At
the classical level, the duality transformation relates the Seiberg-Witten
differentials and spectral curves via a bispectral involution. The quantum
duality extends this to the equivalence of the corresponding Baxter-Schrodinger
equations (quantum spectral curves). This equivalence generalizes both the
spectral self-duality between the 2x2 and NxN representations of the Toda chain
and the famous AHH duality
Catalysis and rotation of F-1 motor: Cleavage of ATP at the catalytic site occurs in 1 ms before 40 degrees substep rotation
F-1, a water-soluble portion of FoF1-ATP synthase, is an ATIP hydrolysis-driven rotary motor. The central gamma-subunit rotates in the alpha(3)beta(3) cylinder by repeating the following four stages of rotation: ATP-binding dwell, rapid 801 substep rotation, interim dwell, and rapid 40degrees substep rotation. At least two 1-ms catalytic events occur in the interim dwell, but it is still unclear which steps in the ATPase cycle, except for ATIP binding, correspond to these events. To discover which steps, we analyzed rotations of F-1 subcomplex (alpha(3)beta(3)gamma) from thermophilic Bacillus PS3 under conditions where cleavage of ATIP at the catalytic site is decelerated: hydrolysis of ATP by the catalytic-site mutant F, and hydrolysis of a slowly hydrolyzable substrate ATPgammaS (adenosine 5'-[gamma-thio]triphosphate) by wild-type F-1. In both cases, interim dwells were extended as expected from bulk phase kinetics, confirming that cleavage of ATP takes place during the interim dwell. Furthermore, the results of ATPgammaS hydrolysis by the mutant F-1 ensure that cleavage of ATIP most likely corresponds to one of the two 1-ms events and not some other faster undetected event. Thus, cleavage of ATP on F-1 occurs in 1 ms during the interim dwell, and we call this interim dwell catalytic dwell
Information heat engine: converting information to energy by feedback control
In 1929, Leo Szilard invented a feedback protocol in which a hypothetical
intelligence called Maxwell's demon pumps heat from an isothermal environment
and transduces it to work. After an intense controversy that lasted over eighty
years; it was finally clarified that the demon's role does not contradict the
second law of thermodynamics, implying that we can convert information to free
energy in principle. Nevertheless, experimental demonstration of this
information-to-energy conversion has been elusive. Here, we demonstrate that a
nonequilibrium feedback manipulation of a Brownian particle based on
information about its location achieves a Szilard-type information-energy
conversion. Under real-time feedback control, the particle climbs up a
spiral-stairs-like potential exerted by an electric field and obtains free
energy larger than the amount of work performed on it. This enables us to
verify the generalized Jarzynski equality, or a new fundamental principle of
"information-heat engine" which converts information to energy by feedback
control.Comment: manuscript including 7 pages and 4 figures and supplementary material
including 6 pages and 8 figure
A 5d/3d duality from relativistic integrable system
We propose and prove a new exact duality between the F-terms of
supersymmetric gauge theories in five and three dimensions with adjoint matter
fields. The theories are compactified on a circle and are subject to the Omega
deformation. In the limit proposed by Nekrasov and Shatashvili, the
supersymmetric vacua become isolated and are identified with the eigenstates of
a quantum integrable system. The effective twisted superpotentials are the
Yang-Yang functional of the relativistic elliptic Calogero-Moser model. We show
that they match on-shell by deriving the Bethe ansatz equation from the saddle
point of the five-dimensional partition function. We also show that the
Chern-Simons terms match and extend our proposal to the elliptic quiver
generalizations.Comment: 30 pages, 4 figures. v2: typo corrected, references adde
Mechanical power in pediatric acute respiratory distress syndrome:a PARDIE study
BACKGROUND: Mechanical power is a composite variable for energy transmitted to the respiratory system over time that may better capture risk for ventilator-induced lung injury than individual ventilator management components. We sought to evaluate if mechanical ventilation management with a high mechanical power is associated with fewer ventilator-free days (VFD) in children with pediatric acute respiratory distress syndrome (PARDS). METHODS: Retrospective analysis of a prospective observational international cohort study. RESULTS: There were 306 children from 55 pediatric intensive care units included. High mechanical power was associated with younger age, higher oxygenation index, a comorbid condition of bronchopulmonary dysplasia, higher tidal volume, higher delta pressure (peak inspiratory pressure—positive end-expiratory pressure), and higher respiratory rate. Higher mechanical power was associated with fewer 28-day VFD after controlling for confounding variables (per 0.1 J·min(−1)·Kg(−1) Subdistribution Hazard Ratio (SHR) 0.93 (0.87, 0.98), p = 0.013). Higher mechanical power was not associated with higher intensive care unit mortality in multivariable analysis in the entire cohort (per 0.1 J·min(−1)·Kg(−1) OR 1.12 [0.94, 1.32], p = 0.20). But was associated with higher mortality when excluding children who died due to neurologic reasons (per 0.1 J·min(−1)·Kg(−1) OR 1.22 [1.01, 1.46], p = 0.036). In subgroup analyses by age, the association between higher mechanical power and fewer 28-day VFD remained only in children < 2-years-old (per 0.1 J·min(−1)·Kg(−1) SHR 0.89 (0.82, 0.96), p = 0.005). Younger children were managed with lower tidal volume, higher delta pressure, higher respiratory rate, lower positive end-expiratory pressure, and higher PCO(2) than older children. No individual ventilator management component mediated the effect of mechanical power on 28-day VFD. CONCLUSIONS: Higher mechanical power is associated with fewer 28-day VFDs in children with PARDS. This association is strongest in children < 2-years-old in whom there are notable differences in mechanical ventilation management. While further validation is needed, these data highlight that ventilator management is associated with outcome in children with PARDS, and there may be subgroups of children with higher potential benefit from strategies to improve lung-protective ventilation. Take Home Message: Higher mechanical power is associated with fewer 28-day ventilator-free days in children with pediatric acute respiratory distress syndrome. This association is strongest in children <2-years-old in whom there are notable differences in mechanical ventilation management. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13054-021-03853-6
Direct Observation of the Myosin Va Recovery Stroke That Contributes to Unidirectional Stepping along Actin
Myosins are ATP-driven linear molecular motors that work as cellular force
generators, transporters, and force sensors. These functions are driven by
large-scale nucleotide-dependent conformational changes, termed
“strokes”; the “power stroke” is the force-generating
swinging of the myosin light chain–binding “neck” domain
relative to the motor domain “head” while bound to actin; the
“recovery stroke” is the necessary initial motion that primes, or
“cocks,” myosin while detached from actin. Myosin Va is a processive
dimer that steps unidirectionally along actin following a “hand over
hand” mechanism in which the trailing head detaches and steps forward
∼72 nm. Despite large rotational Brownian motion of the detached head about
a free joint adjoining the two necks, unidirectional stepping is achieved, in
part by the power stroke of the attached head that moves the joint forward.
However, the power stroke alone cannot fully account for preferential forward
site binding since the orientation and angle stability of the detached head,
which is determined by the properties of the recovery stroke, dictate actin
binding site accessibility. Here, we directly observe the recovery stroke
dynamics and fluctuations of myosin Va using a novel, transient caged
ATP-controlling system that maintains constant ATP levels through stepwise
UV-pulse sequences of varying intensity. We immobilized the neck of monomeric
myosin Va on a surface and observed real time motions of bead(s) attached
site-specifically to the head. ATP induces a transient swing of the neck to the
post-recovery stroke conformation, where it remains for ∼40 s, until ATP
hydrolysis products are released. Angle distributions indicate that the
post-recovery stroke conformation is stabilized by ≥5
kBT of energy. The high kinetic
and energetic stability of the post-recovery stroke conformation favors
preferential binding of the detached head to a forward site 72 nm away. Thus,
the recovery stroke contributes to unidirectional stepping of myosin Va
Hawking Radiation and Tunneling Mechanism for a New Class of Black Holes in Einstein-Gauss-Bonnet Gravity
We study the Hawking radiation in a new class of black hole solutions in the
Einstein-Gauss-Bonnet theory. The black hole has been argued to have vanishing
mass and entropy, but finite Hawking temperature. To check if it really emits
radiation, we analyse the Hawking radiation using the original method of
quantization of scalar field in the black hole background and the quantum
tunneling method, and confirm that it emits radiation at the Hawking
temperature. A general formula is derived for the Hawking temperature and
backreaction in the tunneling approach. Physical implications of these results
are discussed.Comment: 12 pages, v2: Title slightly changed. Motivation and discussions are
elaborated, v3: typos corrected to match the published versio
The practice of child and adolescent psychiatry: a survey of early-career psychiatrists in Japan
<p>Abstract</p> <p>Background</p> <p>Child and adolescent psychiatry (CAP), a subspecialty of psychiatry in Japan, is facing a serious workforce shortage. To resolve this situation, the Japanese government has organized a task force and has been working to increase psychiatrists' clinical skills to improve care for children and adolescents with mental health problems. Using an online questionnaire system, the authors have conducted a survey to investigate the perceptions, experiences, and interests of early-career psychiatrists in CAP.</p> <p>Methods</p> <p>The subjects of this study were 182 psychiatrists in Japan whose individual clinical experiences did not exceed 15 years. The authors of this study created an online questionnaire system and e-mailed the URL and login password to all subjects. Respondents anonymously answered the questions. Most questions required an answer indicating a level of agreement scored on a nine-point scale. Responding to the questionnaire was considered to constitute consent, and all respondents' privacy was carefully protected.</p> <p>Results</p> <p>The mean age and clinical psychiatric experience of the subjects were found to be 33.1 ± 4.5 years and 5.43 ± 3.5 years, respectively. On a nine-point scale (with nine being the highest), experience and interest in CAP measured 3.05 ± 1.9 and 5.34 ± 2.5, respectively; further, these two factors showed significant correlation (r = 0.437, p < 0.0001). The mean score for the early-career psychiatrists' confidence in their ability to diagnose and appropriately treat was notably low, at 3.13 ± 1.9.</p> <p>Conclusion</p> <p>Our results demonstrated that early-career psychiatrists self-evaluated their CAP clinical experience as insufficient, and these clinicians' CAP experiences and interests correlated significantly. Therefore, in order to improve child and adolescent medical care, we need to expose young psychiatrists to sufficient CAP cases and explore the factors that could attract them to this field.</p
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