Nanoparticle image velocimetry at topologically structured surfaces

Nanoparticle image velocimetry ͑nano-PIV͒, based on total internal reflection fluorescent microscopy, is very useful to investigate fluid flows within ϳ100 nm from a surface; but so far it has only been applied to flow over smooth surfaces. Here we show that it can also be applied to flow over a topologically structured surface, provided that the surface structures can be carefully configured not to disrupt the evanescent-wave illumination. We apply nano-PIV to quantify the flow velocity distribution over a polydimethylsiloxane surface, with a periodic gratinglike structure ͑with 215 nm height and 2 m period͒ fabricated using our customized multilevel lithography method. The measured tracer displacement data are in good agreement with the computed theoretical values. These results demonstrate new possibilities to study the interactions between fluid flow and topologically structured surfaces

Monitoring the orientation of rare-earth-doped nanorods for flow shear tomography

Rare-earth phosphors exhibit unique luminescence polarization features originating from the anisotropic symmetry of the emitter ion's chemical environment. However, to take advantage of this peculiar property, it is necessary to control and measure the ensemble orientation of the host particles with a high degree of precision. Here, we show a methodology to obtain the photoluminescence polarization of Eu-doped LaPO4 nano rods assembled in an electrically modulated liquid-crystalline phase. We measure Eu3+ emission spectra for the three main optimal configurations ({\sigma}, {\pi} and {\alpha}, depending on the direction of observation and the polarization axes) and use them as a reference for the nano rod orientation analysis. Based on the fact that flowing nano rods tend to orient along the shear strain profile, we use this orientation analysis to measure the local shear rate in a flowing liquid. The potential of this approach is then demonstrated through tomographic imaging of the shear rate distribution in a microfluidic system.Comment: 8 pages, 3 figures + supplementary files for experimental and numerical method

In vivo whole-field blood velocity measurement techniques

Mechanical, Maritime and Materials Engineerin

Tapered microfluidic chip for the study of biochemical and mechanical response at subcellular level of endothelial cells to shear flow

A lab-on-a-chip application for the investigation of biochemical and mechanical response of individual endothelial cells to different fluid dynamical conditions is presented. A microfluidic flow chamber design with a tapered geometry that creates a pre-defined, homogeneous shear stress gradient on the cell layer is described and characterized. A non-intrusive, non-tactile measurement method based on micro-PIV is used for the determination of the topography and shear stress distribution over individual cells with subcellular resolution. The cellular gene expression is measured simultaneously with the shape and shear stress distribution of the cell. With this set-up the response of the cells on different pre-defined shear stress levels is investigated without the influence of variations in repetitive experiments. Results are shown on cultured endothelial cells related to the promoter activity of the shear-responsive transcription factor KLF2 driving the marker gene for green fluorescent protein. © The Royal Society of Chemistry 2009

Digital holographic microscopy applied to measurement of a flow in a T-shaped micromixer

In this paper, we describe measurements of a three-dimensional (3D) flow in a T-shaped micromixer by means of digital holographic microscopy. Imaging tracer particles in a microscopic flow with conventional microscopy is accompanied by a small depth-of-field, which hinders true volumetric flow measurements. In holographic microscopy, the depth of the measurement domain does not have this limitation because any desired image plane can be reconstructed after recording. Our digital holographic microscope (DHM) consists of a conventional in-line recording system with an added magnifying optical element. The measured flow velocity and the calculated vorticity illustrate four streamwise vortices in the micromixer outflow channel. Because the investigated flow is stationary and strongly 3D, the DHM performance (i.e. accuracy and resolution) can be precisely investigated. The obtained Dynamic spatial range and Dynamic velocity range are larger than 20 and 30, respectively. High-speed multiple-frame measurements illustrate the capability to simultaneously track about 80 particles in a volumetric measurement domain.Process and EnergyMechanical, Maritime and Materials Engineerin