Characterisation of the immune response to the UK human anthrax vaccine

The UK human anthrax vaccine consists of the alum-precipitated culture supernatant of Bacillus anthracis Sterne. In addition to protective antigen (PA), the key immunogen, the vaccine also contains a number of other bacteria- and media-derived proteins. These proteins may contribute to the transient side effects experienced by some individuals and could influence the development of the PA-specific immune response. Bacterial cell-wall components have been shown to be potent immunomodulators. B. anthracis expresses two S-layer proteins, EA1 and Sap, which have been demonstrated to be immunogenic in animal studies. These are also immunogenic in man so that convalescent and post-immunisation sera contain specific antibodies to Ea1, and to a lesser extent, to Sap. To determine if these proteins are capable of modifying the protective immune response to PA, A/J mice were immunised with equivalent amounts of recombinant PA and S-layer proteins in the presence of alhydrogel. IgG isotype profiles were determined and the animals were subsequently challenged with spores of B. anthracis STI. The results suggest that there was no significant shift in IgG isotype profile and that the presence of the S-layer proteins did not adversely affect the protective immune response induced by PA

The Double Binds of Indigeneity and Indigenous Resistance

During the twentieth century, indigenous peoples have often embraced the category of indigenous while also having to face the ambiguities and limitations of this concept. Indigeneity, whether represented by indigenous people themselves or others, tends to face a “double bind”, as defined by Gregory Bateson, in which “no matter what a person does, he can’t win.” One exit strategy suggested by Bateson is meta-communication—communication about communication—in which new solutions emerge from a questioning of system-internal assumptions. We offer case studies from Ecuador, Peru and Alaska that chart some recent indigenous experiences and strategies for such scenarios

Collaborative Negotiations a Successful Approach for Negotiating Compliance Milestones for the Transition of the Plutonium Finishing Plant (PFP), Hanford Nuclear Reservation, and Hanford, Washington

The Hanford Nuclear Reservation is a former U. S. Department of Energy Defense Production Site. The site is currently listed on the National Priorities List of the Comprehensive Environmental Response Compensation and Liability Act of 1980 (CERCLA) and is undergoing cleanup and environmental restoration. The PFP is a former Plutonium metal production facility. The operating mission of the PFP ended with a DOE Headquarters shutdown letter in October of 1996. Generally, the receipt of a shutdown letter initiates the start of Transition (as the first step of Decommissioning) of a facility. The Hanford site is subject to the Hanford Federal Facilities Compliance Act and Consent Order (HFFCCO), an order on consent signed by the DOE, the U. S. Environmental Protection Agency, (EPA) and the Washington Department of Ecology (WDOE). Under the HFFCCO, negotiations for transition milestones begin within six months after the issuance of a shutdown order. In the case of the PFP, the Nuclear Materials disposition and stabilization activities, a DOE responsibility, were necessary as precursor activities to Transition. This situation precipitated a crisis in the negotiations between the agencies, and formal negotiations initiated in 1997 ended in failure. The negotiations reached impasse on several key regulatory and operational issues. The 1997 negotiation was characterized by a strongly positional style. DOE and the regulatory personnel took hard lines early in the negotiations and were unable to move to resolution of key issues after a year and a half. This resulted in unhappy stakeholders, poor publicity and work delays as well as wounded relationships between DOE and the regulatory community. In the 2000-2001 PFP negotiations, a completely different approach was suggested and eventually initiated: Collaborative Negotiations. The collaborative negotiation style resulted in agreement between the agencies on all key issues within 6 months of initiation. All parties were very pleased with the results and all parties were relieved that protracted negotiations sessions were not needed with the new style of working together collaboratively to serve each other's interests. The characteristics of collaborative negotiations included building trust, emphasizing equality of team members, problem solving by the entire team, relying on individual communications and self-management skills. The team found that trust building sessions were key to successfully working through issues. Relationship differences were too often ignored in the past negotiations and were recognized and worked through in the collaborative process

Disaster, Degradation, Dystopia

In this chapter we examine the contributions that the field of political ecology––with its focus on the mutually constitutive relationships between environments, cultures, politics and power––has made, and can continue to make, to a more nuanced understanding of disasters. Disaster research also contributes to political ecology insofar as it illuminates the complexity of relationships between environments and societies over space and time. Drawing from ethnographic examples and historical analysis, we situate epistemologies of disasters within broader analyses of scale-making, nature–culture dichotomies, the classification of disasters as ‘natural’ or ‘social’, the interpretive dimensions of identity and the construction of self. The very definition of a situation as ‘disastrous’ or not varies with one’s political resources. Overall, we argue that political ecology frameworks pose new questions about the operation of power and politics in contexts of disasters, resulting in enriched understandings of the social experience of disasters. Ethnographic examples, such as those presented in this chapter, illustrate the rich promise of continued work at the confluence of the fields of political ecology and disaster studies

Probabilistic fatigue S-N curves derivation for notched components

Europe has a number of ancient riveted metallic bridges, constructed during the second half of the 19th century up to the middle of the 20th century, which are still in operation. In this paper, a unified approach is presented to generate probabilistic S-N curves to be applied to structural components, accounting for uncertainties in material properties. The approach is particularly demonstrated for a plate with a circular hole, made of puddle iron from the Portuguese Eiffel Bridge. This paper presents an extension of the local strain-based fatigue crack propagation model proposed by Noroozi et al. The latter model is applied to derive the probabilistic fatigue crack propagation field (p-S-Np field). The probabilistic fatigue crack initiation field (p-S-Ni field) is determined using a notch elastoplastic approach, to calculate the fatigue failure of the first elementary material block ahead of the notch roo