An Injectable Trace Mineral Supplement in Yearling Bulls Causes a Short-Term Increase in Circulating Trace Mineral Levels But Does Not Improve Sperm Quality

Proper trace mineral supplementation is necessary for reproductive development and function. Supplementation with various trace minerals has been shown to improve overall sperm quality and morphology and increase the percentage of live sperm. When developing beef bulls, it is necessary to meet trace mineral requirements to ensure proper reproductive success. An injectable trace mineral product has been made commercially available for use in cattle as a supplemental form of chelated selenium, copper, zinc, and manganese. Considering the role of trace minerals in bull reproductive function, we posed the question of whether using an injectable trace mineral product beyond dietary supplementation could improve sperm quality and percentage of bulls passing yearling breeding soundness exams. The objectives of our study were to compare serum trace mineral concentrations of bulls before and after administration of an injection of trace minerals or saline and to compare semen trace mineral concentrations after treatment. To determine if an injectable trace mineral product could be of benefit, we compared serum and semen trace mineral concentrations as well as semen quality and percentage passing a yearling breeding soundness examination in treated and untreated bulls

MEASUREMENTS OF BUCKLINGS AND DIFFUSION COEFFICIENT CHANGES IN Dsub2sub 2O- MODERATED LATTICES OF TUBULAR UOsub2sub 2 ASSEMBLIES

Diffusion coefficients and bucklings are measured for UO/sub 2/ fuel tube assemblies in D/sub 2/O moderated lattices. The effects of H/sub 2/O fogs and air in the coolant channels in place of D/sub 2/O are studied. (T.F.H.

Oxidation of the Ediacaran Ocean

Oxygenation of the Earth's surface is increasingly thought to have occurred in two steps. The first step, which occurred ~2,300 million years (Myr) ago, involved a significant increase in atmospheric oxygen concentrations and oxygenation of the surface ocean. A further increase in atmospheric oxygen appears to have taken place during the late Neoproterozoic period (~800–542 Myr ago). This increase may have stimulated the evolution of macroscopic multicellular animals and the subsequent radiation of calcified invertebrates, and may have led to oxygenation of the deep ocean. However, the nature and timing of Neoproterozoic oxidation remain uncertain. Here we present high-resolution carbon isotope and sulphur isotope records from the Huqf Supergroup, Sultanate of Oman, that cover most of the Ediacaran period (~635 to ~548 Myr ago). These records indicate that the ocean became increasingly oxygenated after the end of the Marinoan glaciation, and they allow us to identify three distinct stages of oxidation. When considered in the context of other records from this period, our data indicate that certain groups of eukaryotic organisms appeared and diversified during the second and third stages of oxygenation. The second stage corresponds with the Shuram excursion in the carbon isotope record and seems to have involved the oxidation of a large reservoir of organic carbon suspended in the deep ocean, indicating that this event may have had a key role in the evolution of eukaryotic organisms. Our data thus provide new insights into the oxygenation of the Ediacaran ocean and the stepwise restructuring of the carbon and sulphur cycles that occurred during this significant period of Earth's history

Different histories but similar genetic diversity and structure for black walnut in Indiana and Missouri

—Missouri and Indiana have markedly different histories of glaciation and recolonization by forest trees. These states also differ in land use patterns and degree of anthropogenic landscape change such as forest fragmentation. To determine the overall effects of these and other demographic differences on the levels of genetic diversity and structure in black walnut (Juglans nigra L.) more than 550 total black walnut trees from nine populations in Indiana and 10 in Missouri were sampled and analyzed using 12 nuclear microsatellite loci. Although genetic diversity parameters such as allelic richness and expected heterozygosity were high overall, they varied little among populations and their mean values for the two states were not significantly different. Pairwise genetic distance values between all population pairs ranged from 0.012-0.159, but no significant pattern of isolation by distance was detected. The estimate of the degree of genetic differentiation between states (FPT = 0.0009) was very small and not significant, indicating that differences between states explained an inconsequential portion of the total variance. The observed low levels of local and regional genetic structure indicate that high levels of pollen flow have buffered black walnut from the genetic consequences of founder effects and genetic drift in both geologic and recent time scales

Meralgia paresthetica after “all-in-one” appendectomy

AbstractMinimally invasive approaches have become standard for pediatric appendectomy. The laparoscopic assisted single port approach, also known as the “all-in-one” appendectomy, has gained recent popularity [1]. We describe a child who suffered meralgia paresthetica (a neuropathy in the distribution of the lateral femoral cutaneous nerve) after a laparoscopic assisted single port appendectomy, perhaps secondary to mobilization of the cecum

Carbon Isotope and Lipid Biomarker Stratigraphy from Organic-Rich Strata Through the Neoproterozoic Shuram Excursion in South Oman

The regulation of oxygen levels in Earth’s atmosphere and oceans is inextricably linked to the carbon cycle. Carbon isotope ratios of carbonate and sedimentary organic matter provide first order insights into the operation of the carbon cycle in the geologic past. During the Ediacaran period, the ~580 Ma ‘Shuram Excursion’ (SE) records a dramatic, systematic shift in δ^(13)C_(carbonate) values to as low as cɑ. -12‰, lasting potentially millions to tens of millions of years in duration and constitutes the largest carbon isotope excursion known in the record [1]. The extremely negative carbon isotope values in carbonate challenges our understanding of the ancient carbon cycle and is difficult to rationalise via uniform carbon cycle principles. Several hypotheses have been developed to explain this behaviour, all of which make different predictions for the abundance, structure, and isotopic composition of organic carbon through the excursion. For a direct test of these ideas, we report paired organic and inorganic stable carbon isotope ratios in addition to detailed lipid biomarker stratigraphic records from a subsurface well drilled on the eastern flank of the South Oman Salt Basin, Sultanate of Oman. This well captures thermally immature and organic-rich Nafun Group strata traversing the SE, yielding variable but primary biomarker characteristics typical of Neoproterozoic rocks from this region. Despite the high organic matter contents, the carbon isotopic compositions of carbonates do not covary with those of organic phases. Furthermore, lipid biomarker data reveal that organic matter composition and source inputs varied stratigraphically, reflecting biological community shifts in non-migrated, syngenetic organic matter deposited during this interval. Together these observations imply that carbonateorganic isotopic decoupling during the SE is not a result of mixing of fossil or exogenous carbon sources (either DOC, detrital, or migrated) with syngenetic organic matter

Assessment of Novel Semen Evaluation Technologies and Breed Comparisons in Yearling Beef Bulls

Objective: The objectives were 1) to evaluate the iSperm, when conducting breeding soundness exams (BSE) on bulls by comparing sperm motility to a technician’s assessment and 2) to evaluate correlations between sperm response to reactive oxygen species (ROS) and functional sperm measurements. Study Description: Ejaculates were collected via electroejaculation from yearling bulls as part of a BSE. All BSE were conducted by one veterinarian and ejaculates were evaluated by a single technician. Additional sperm motility analysis was conducted with the iSperm analyzer. Ejaculates meeting minimum thresholds for passing a BSE were diluted and sent overnight for flow cytometry evaluation. Data were analyzed using Pearson’s correlation coefficients in SAS. Results: Both gross and progressive motilities were significantly (r = 0.30; 0.38; P \u3c 0.001) correlated to the technician’s assessment of progressive motility. Percentage of live spermatozoa with positive ROS status was correlated (r = 0.53; P \u3c 0.001) with percentage progressive motility. Percentage of live spermatozoa with negative ROS status was moderately correlated with percentage spermatozoa exhibiting secondary abnormalities (r = 0.33; P = 0.02). Percentage live spermatozoa that had disrupted acrosomes was strongly correlated with percentage live spermatozoa with negative ROS (r = 0.66; P \u3c 0.001) and moderately negatively correlated with percentage live spermatozoa with positive ROS (r = -0.31; P = 0.04). Percentage of live spermatozoa with positive ROS status was correlated (r = 0.58; P \u3c 0.001) with percentage of spermatozoa with active mitochondrial membranes. Percentage of live spermatozoa with positive ROS status was strongly correlated (r = 0.92; P \u3c 0.001) with percentage of live spermatozoa with intact acrosomes. The Bottom Line: The iSperm can be used to produce semen assessments similar to those of a trained technician and may offer a useful tool for producers to perform on-farm semen analysis. Sperm health and function continue to be related to negative ROS status

Sire Distribution of Calves in a Beef Herd with Use of Fixed Time Artificial Insemination Followed by Immediate Bull Exposure for Natural Service in Cows and Heifers

Objective: Our objective was to determine the relative percentages of calves sired by either natural service sire or fixed time artificial insemination (FTAI) sire within the same estrous period. Study Description: During two consecutive years, heifers and cows were synchronized and inseminated using the 7-day CO-Synch + CIDR FTAI protocol. All females were exposed to natural service bulls immediately following insemination. After calving, DNA was collected from a random subset of calves born in the first 21 days of the calving season for parentage analysis. Calves born from heifers totaled 59 in Year 1 and 82 in Year 2; calves born from cows totaled 89 in Year 1 and 102 in Year 2. Results: In Year 1, among calves born from heifers, the percentage sired by natural service was 5.1% (n = 3/59). Among calves born from cows, the percentage sired by natural service was 14.6% (n = 13/89). In Year 2, among calves born from heifers, the percentage sired by natural service was 9.8% (n = 8/82). Among calves born from cows, the percentage sired by natural service was 20.6% (n = 21/102). The Bottom Line: If commercial producers use FTAI followed by immediate bull exposure in beef females, it can be expected that natural service bulls may sire 5 to 20% of calves born early in the calving season while reducing time and labor associated with bull turnout

Switchgrass Response to Nitrogen Fertilizer Across Diverse Environments in the USA: a Regional Feedstock Partnership Report

The Regional Feedstock Partnership is a collaborative effort between the Sun Grant Initiative (through Land Grant Universities), the US Department of Energy, and the US Department of Agriculture. One segment of this partnership is the field-scale evaluation of switchgrass (Panicum virgatum L.) in diverse sites across the USA. Switchgrass was planted (11.2 kg PLS ha−1 ) in replicated plots in New York, Oklahoma, South Dakota, and Virginia in 2008 and in Iowa in 2009. Adapted switchgrass cultivars were selected for each location and baseline soil samples collected before planting. Nitrogen fertilizer (0, 56, and 112 kg N ha−1 ) was applied each spring beginning the year after planting, and switchgrass was harvested once annually after senescence. Establishment, management, and harvest operations were completed using fieldscale equipment. Switchgrass production ranged from 2 to 11.5 Mg ha−1 across locations and years. Yields were lowest the first year after establishment. Switchgrass responded positively to N in 6 of 19 location/year combinations and there was one location/year combination (NY in Year 2) where a significant negative response was noted. Initial soil N levels were lowest in SD and VA (significant N response) and highest at the other three locations (no N response). Although N rate affected some measures of biomass quality (N and hemicellulose), location and year had greater overall effects on all quality parameters evaluated. These results demonstrate the importance of local field-scale research and of proper N management in order to reduce unnecessary expense and potential environmental impacts of switchgrass grown for bioenergy

Delayed Timing of Insemination Relative to Estrus Improves Pregnancy to Artificial Insemination With Sex-Sorted Semen in Beef Heifers

Objective: The objective was to evaluate the effect of timing of artificial insemination (AI) relative to the onset of estrus on pregnancy outcome when using sex-sorted semen in beef heifers. Study Description: Beef heifers were subjected to the melengestrol acetate with prostaglandin (MGA-PG) estrous synchronization protocol and visually observed for estrus every four hours for five days following injection of PG. Following detection of estrus, heifers were inseminated with semen sorted to contain X-chromosome bearing sperm cells (4.0 × 106 live cells per 0.25 mL straw of SexedULTRA 4M). Heifers were retrospectively categorized into one of three intervals from estrus onset to insemination: 1) 12.5–15.9 hours; 2) 16.5–21.0 hours; and 3) 21.4–27.5 hours. Results: Heifers with the shortest interval (12.5–15.9 hours) from estrus onset to insemination had a similar (P \u3e 0.10) AI pregnancy rate as compared with heifers with the interval from estrus onset to insemination of 16.5 to 21 hours. Heifers inseminated 21.4 to 27.5 hours following estrus onset achieved a greater (P ≤ 0.05) AI pregnancy rate than heifers inseminated 12.5 to 15.9 hours following estrus onset. The Bottom Line: Insemination of beef heifers with sex-sorted semen later than 21 hours after estrus onset appears to improve pregnancy rate to AI when compared to earlier insemination times