All-optical endoscopic probe for high resolution 3D photoacoustic tomography

A novel all-optical forward-viewing photoacoustic probe using a flexible coherent fibre-optic bundle and a Fabry- Perot (FP) ultrasound sensor has been developed. The fibre bundle, along with the FP sensor at its distal end, synthesizes a high density 2D array of wideband ultrasound detectors. Photoacoustic waves arriving at the sensor are spatially mapped by optically scanning the proximal end face of the bundle in 2D with a CW wavelength-tunable interrogation laser. 3D images are formed from the detected signals using a time-reversal image reconstruction algorithm. The system has been characterized in terms of its PSF, noise-equivalent pressure and field of view. Finally, the high resolution 3D imaging capability has been demonstrated using arbitrary shaped phantoms and duck embryo

Multi-scale analysis of compressible viscous and rotating fluids

We study a singular limit for the compressible Navier-Stokes system when the Mach and Rossby numbers are proportional to certain powers of a small parameter \ep. If the Rossby number dominates the Mach number, the limit problem is represented by the 2-D incompressible Navier-Stokes system describing the horizontal motion of vertical averages of the velocity field. If they are of the same order then the limit problem turns out to be a linear, 2-D equation with a unique radially symmetric solution. The effect of the centrifugal force is taken into account

Real-time and Freehand Multimodal Imaging: Combining White Light Endoscopy with All-Optical Ultrasound

Minimally invasive surgery offers significant benefits over open surgery in terms of patient recovery, complication rates, and cost. Accurate visualisation is key for successful interventions; however, no single imaging modality offers sufficient resolution, penetration, and soft-tissue contrast to adequately monitor interventional treatment. Consequently, multimodal interventional imaging is intensively investigated. All-optical ultrasound (AOUS) imaging is an emerging modality where light is used to both generate and detect ultrasound. Using fibre-optics, highly miniaturised imaging probes can be fabricated that yield high-quality pulse-echo images and are readily integrated into minimally invasive interventional instruments. In this work, we present the integration of a miniature (diameter: 800 µm), highly directional AOUS imaging probe into a commercially available white light urethroscope, and demonstrate the first real-time, 3D multimodal imaging combining AOUS and white light endoscopy. Through the addition of an electromagnetic tracker, the position and pose of the instrument could be continuously recorded. This facilitated accurate real-time registration of the imaging modalities, as well as freehand operation of the instrument. In addition, the freehand imaging paradigm allowed for “piece-wise” scanning where the instrument was retracted and repositioned without recalibration. The presented imaging probe and system could significantly improve the quality of image guidance during interventional surgery

Field spectrometer to measure percent ground coverage and leaf area index of agriculture crops

Measurement of percent ground cover (PGC) and leaf area index (LAI) are required for crop modelling, yield estimation and for ground truth data in remote sensing studies. An instrument which traverses on a track above a crop and continuously measures the ratio of incident and reflected radiation at various wavelengths was developed and tested. Spectral irradiance and reflectance measurements were made at four wavelengths (647.8, 675.5, 739.9, 790.4 nm) at eight stages of growth of wheat and barley for three crop densities. Data provided information on the relations between spectral properties at selected wavelength and leaf area expansion at different growth stages. The spectral data were highly correlated with leaf area index measurements

Variations of crop canopy spectral reflectance measurements under changing sky conditions

The possibility of using reflectance data collected under both cloudy and sunny conditions is studied. Extrenle values of red and far-red reflectances measured directly over bare soil and crop canopies during intermittently cloudy conditions can differ by more than 100 percent and 60 percent, respectively. Eliminating the irradiance variations by using only data collected under sunny conditions reduced such variation to within 1 percent for bare soil and to within 8 percent and 20 percent for crop canopy red and far-red reflectances, respectively. In general, the reflectances measured under cloudy conditions with relatively constant irradiance values are constant and approximately 10 percent larger than the ones measured at similar sun angles during sunny conditions. This result is independent of the species and the spectral regions under study

FUM1—A Gene Required for Fumonisin Biosynthesis But Not for Maize Ear Rot and Ear Infection by Gibberella moniliformis in Field Tests

We have analyzed the role of fumonisins in infection of maize (Zea mays) by Gibberella moniliformis (anamorph Fusarium verticillioides) in field tests in Illinois and Iowa, United States. Fumonisin-nonproducing mutants were obtained by disrupting FUM1 (previouslyFUM5), the gene encoding a polyketide synthase required for fumonisin biosynthesis. Maize ear rot, ear infection, and fumonisin contamination were assessed by silk-channel injection in 1999 and 2000 and also by spray application onto maize silks, injection into maize stalks, and application with maize seeds at planting in 1999. Ear rot was evaluated by visual assessment of whole ears and by calculating percentage of symptomatic kernels by weight. Fumonisin levels in kernels were determined by high-performance liquid chromatography. The presence of applied strains in kernels was determined by analysis of recovered isolates for genetic markers and fumonisin production. Two independent fumonisin-nonproducing (fum1-3 and fum1-4) mutants were similar to their respective fumonisin-producing (FUM1-1) progenitor strains in ability to cause ear rot following silk-channel injection and also were similar in ability to infect maize ears following application by all four methods tested. This evidence confirms that fumonisins are not required for G. moniliformis to cause maize ear rot and ear infection

Field spectrometer to measure percent ground coverage and leaf area index of agriculture crops [remote sensing]

Measurement of percent ground cover (PGC) and leaf area index (LAI) are required for crop modelling, yield estimati-on and for ground truth data in remote sensing studies. An instrument which traverses on a track above a crop and continuously measures the ratio of incident and reflected radiation at various wavelengths was developed and tested. Spectral irradiance and reflectance measurements were made at four wavelengths (647.8 , 675.5 , 739.9 , 790.4 nm) at eight stages of growth of wheat and barley for three crop densities. Data provided information on the relations between spectral properties at selected wavelength and leaf area expansion at different growth stages. The spectral data were highly correlated with leaf area index measurements

Variabilité introduite dans des mesures de réflectances spectrales de couverts végétaux agricoles par les facteurs état du ciel et vent

Parmi les facteurs introduisant de la variabilité indésirable dans les mesures de réflectance spectrale prises au-dessus de couverts végétaux, ceux de type radiatif ont été moins étudiés. A l'aide d'expériences isolant certains de ces facteurs et de méthodes empiriques d'analyses, nos résultats montrent que la présence de radiation diffuse non-directionnelle dans la radiation solaire incidente n'introduit pas beaucoup de variabilité indésirable dans les mesures de réflectance spectrale. Ils montrent aussi que la présence de nuages vis-à-vis le disque solaire entraîne généralement une sous-estimation des réflectances mesurées sous ces conditions par rapport aux conditions de ciel serein. Il semble toutefois possible de corriger cette erreur et ainsi d'utiliser indifféremment l'une ou l'autre de ces deux conditions radiatives pour relier les mesures de réflectances spectrales prises au niveau du sol à des paramètres du couvert végétal. Le vent est un autre facteur qui introduit passablement de variabilité indésirable dans ces mesures. Si la structure du couvert, la région spectrale et la vitesse du vent font varier l'importance de ce facteur, il n'en demeure pas moins que ces fluctuations continuelles dans le temps peuvent conduire à mesurer des réflectances qui ne sont pas représentatives de la réflectance réelle du couvert végétal au moment de la mesure. Many factors including the radiative ones introduce indesirable variability in measurements of spectral reflectance. Based upon experimental studies related to one specific factor at a time and a1so on empirica1 method of analysis, our results show that non-directional diffuse radiation from incident solar radiation introduce little indesirable variability in spectral reflectance measurements. They also show that clouds hiding the solar disc 1ead usually to an underestimation of reflectances measured under these conditions compared to those measured under clear sky. However, it seems easy to account for this difference. It then implies that the measurement of the spectral reflectances at the ground level under either one of these radiative conditions will give values which will be possible to relate to plant canopy structure

Patient-specific polyvinyl alcohol phantom fabrication with ultrasound and x-ray contrast for brain tumor surgery planning

Phantoms are essential tools for clinical training, surgical planning and the development of novel medical devices. However, it is challenging to create anatomically accurate head phantoms with realistic brain imaging properties because standard fabrication methods are not optimized to replicate any patient-specific anatomical detail and 3D printing materials are not optimized for imaging properties. In order to test and validate a novel navigation system for use during brain tumor surgery, an anatomically accurate phantom with realistic imaging and mechanical properties was required. Therefore, a phantom was developed using real patient data as input and 3D printing of molds to fabricate a patient-specific head phantom comprising the skull, brain and tumor with both ultrasound and X-ray contrast. The phantom also had mechanical properties that allowed the phantom tissue to be manipulated in a similar manner to how human brain tissue is handled during surgery. The phantom was successfully tested during a surgical simulation in a virtual operating room. The phantom fabrication method uses commercially available materials and is easy to reproduce. The 3D printing files can be readily shared, and the technique can be adapted to encompass many different types of tumor