Ongoing over-exploitation and delayed responses to environmental change highlight the urgency for action to promote vertebrate recoveries by 2030

To safeguard nature, we must understand the drivers of biodiversity loss. Time-delayed biodiversity responses to environmental changes (ecological lags) are often absent from models of biodiversity change, despite their well-documented existence. We quantify how lagged responses to climate and land-use change have influenced mammal and bird populations around the world, while incorporating effects of direct exploitation and conservation interventions. Ecological lag duration varies between drivers, vertebrate classes and body size groupings-e.g. lags linked to climate-change impacts are 13 years for small birds, rising to 40 years for larger species. Past warming and land conversion generally combine to predict population declines; however, such conditions are associated with population increases for small mammals. Positive effects of management (>+4% annually for large mammals) and protected areas (>+6% annually for large birds) on population trends contrast with the negative impact of exploitation (<-7% annually for birds), highlighting the need to promote sustainable use. Model projections suggest a future with winners (e.g. large birds) and losers (e.g. medium-sized birds), with current/recent environmental change substantially influencing abundance trends to 2050. Without urgent action, including effective conservation interventions and promoting sustainable use, ambitious targets to stop declines by 2030 may already be slipping out of reach

ArDM: a ton-scale liquid Argon experiment for direct detection of Dark Matter in the Universe

The ArDM project aims at developing and operating large noble liquid detectors to search for direct evidence of Weakly Interacting Massive Particle (WIMP) as Dark Matter in the Universe. The initial goal is to design, assemble and operate a $\approx$1 ton liquid Argon prototype to demonstrate the feasibility of a ton-scale experiment with the required performance to efficiently detect and sufficiently discriminate backgrounds for a successful WIMP detection. Our design addresses the possibility to detect independently ionization and scintillation signals. In this paper, we describe this goal and the conceptual design of the detector.Comment: 5 pages, 3 figures, Talk given at IXth international conference on Topics in Astroparticle and Underground Physics (TAUP05), Zaragoza, (Spain

A model for uranium mobility in groundwater in the Grand Pré region, Nova Scotia, Canada

Elevated levels of uranium that surpass maximum allowable concentrations have been recognized in some well water samples throughout Nova Scotia. In this study we examine the influence of redox conditions and regional geology on the mobility of uranium in groundwater in the Grand Pré region, located in the eastern end of the Annapolis-Cornwallis Valley in southwestern Nova Scotia. The study site is underlain by carbon-rich shale and arkosic sandstone of the Late Mississippian Horton Bluff Formation and the arenaceous sandstone of the Triassic Wolfville Formation. Water samples from drilled wells were analyzed on site for various water quality parameters (pH, conductivity, temperature, dissolved oxygen) and in the laboratory to determine alkalinity and cation (72) and major anion (9) concentrations. Several samples exceeded Health Canada (2011) limits for uranium (20 μg/L) with values up to 50.8 μg/L and aesthetic objectives (AO) for iron (up to 605 μg/L), manganese (up to 2.28 mg/L) and other cations. Data show that different redox conditions occur in the study area. The geospatial arrangement of the chemical data suggests that the lithology of the underlying aquifer strongly influences the availability of uranium in groundwater. Reduced groundwater in Horton Bluff sandstone typically contains elevated concentrations of Fe 2+ and Mn 2+ , whereas groundwater containing high concentrations of U is found primary in oxidizing environments in quartz arenite sandstone units in the Wolfville Formation. Consumption of groundwater in the Grand Pré region may pose a health risk; however, the level of risk remains unknown. This study emphasizes that in the area of Grand Pré, water that is considered aesthetically undesirable (containing high Fe and Mn) may pose less of a health risk (low U) than waters that are aesthetically pleasing.Des niveaux élevés d’uranium qui dépassent les concentrations maximales admises ont été détectés dans certains échantillons d’eau de puits en Nouvelle Écosse. Dans cette étude, nous examinons les effets de la réduction-oxydation et de la géologie régionale sur la mobilité de l’uranium dans l’eau souterraine de la région de Grand Pré, dans l’extrémité est de la vallée d’Annapolis-Cornwallis, dans le sud ouest de la Nouvelle Écosse. Le site étudié repose sur du schiste charbonneux et du grès arkosique de la Formation de Horton Bluff, du Mississippien tardif, et sur du grès arénacé de la Formation de Wolfville, du Trias. Les échantillons d’eau de puits creusés ont été analysés sur place pour ce qui est de divers paramètres de qualité de l’eau (pH, conductivité, température, oxygène dissous) et des analyses ont eu lieu en laboratoire pour établir l’alcalinité et les concentrations de cations (72) et de principaux anions (9). Plusieurs échantillons dépassaient les limites permises par Santé Canada (2011), en ce qui concerne la teneur en uranium (20 μg/L), certaines teneurs ayant atteint 50,8 μg/L. Il en allait de même en ce qui concerne les critères esthétiques pour le fer (des teneurs ayant atteint 605 μg/L), le manganèse (des teneurs ayant atteint 2,28 mg/L) et d’autres cations. Les données indiquent que divers phénomènes de réduction-oxydation surviennent dans le secteur étudié. Le contexte géospatial des données chimiques porte à croire que la lithologie de la couche aquifère sous jacente exerce une forte influence sur la disponibilité de l’uranium dans l’eau souterraine. L’eau souterraine qui a subi une réduction dans le grès de Horton Bluff contient généralement des concentrations élevées de Fe 2+ et de Mn 2+ , tandis que l’eau souterraine à forte concentration d’uranium est surtout observée dans les milieux oxydants de grès de quartz-arénite de la Formation de Wolfville. La consommation de l’eau souterraine dans la région de Grand Pré peut poser un risque pour la santé, mais le degré de risque demeure inconnu. Cette étude met en lumière le risque moindre pour la santé (faible teneur en U) de l’eau dans la région de Grand Pré, qui a une apparence pouvant laisse à désirer (elle a une forte teneur en Fe et en Mn), comparativement à d’autres sources d’eau d’apparence plus agréable. [Traduit par la redaction

Longitudinal patterns in an Arkansas River Valley stream: an Application of the River Continuum Concept

The River Continuum Concept (RCC) provides the framework for studying how lotic ecosystems vary from headwater streams to large rivers. The RCC was developed in streams in eastern deciduous forests of North America, but watershed characteristics and land uses differ across ecoregions, presenting unique opportunities to study how predictions of the RCC may differ across regions. Additionally, RCC predictions may vary due to the influence of fishes, but few studies have used fish taxa as a metric for evaluating predictions of the RCC. Our goal was to determine if RCC predictions for stream orders 1 through 5 were supported by primary producer, macroinvertebrate, and fish communities in Cadron Creek of the Arkansas River Valley. We sampled chlorophyll a, macroinvertebrates, and fishes at five stream reaches across a gradient of watershed size. Contrary to RCC predictions, chlorophyll a did not increase in concentration with catchment size. As the RCC predicts, fish and macroinvertebrate diversity increased with catchment size. Shredding and collecting macroinvertebrate taxa supported RCC predictions, respectively decreasing and increasing in composition as catchment area increased. Herbivorous and predaceous fish did not follow RCC predictions; however, surface-water column feeding fish were abundant at all sites as predicted. We hypothesize some predictions of the RCC were not supported in headwater reaches of this system due to regional differences in watershed characteristics and altered resource availability due to land use surrounding sampling sites

Synaptic tagging and capture : differential role of distinct calcium/calmodulin kinases in protein synthesis-dependent long-term potentiation

Weakly tetanized synapses in area CA1 of the hippocampus that ordinarily display long-term potentiation lasting ~3 h (called early-LTP) will maintain a longer-lasting change in efficacy (late-LTP) if the weak tetanization occurs shortly before or after strong tetanization of an independent, but convergent, set of synapses in CA1. The synaptic tagging and capture hypothesis explains this heterosynaptic influence on persistence in terms of a distinction between local mechanisms of synaptic tagging and cell-wide mechanisms responsible for the synthesis, distribution, and capture of plasticity-related proteins (PRPs). We now present evidence that distinct CaM kinase (CaMK) pathways serve a dissociable role in these mechanisms. Using a hippocampal brain-slice preparation that permits stable long-term recordings in vitro for >10 h and using hippocampal cultures to validate the differential drug effects on distinct CaMK pathways, we show that tag setting is blocked by the CaMK inhibitor KN-93 (2-[N-(2-hydroxyethyl)]-N-(4-methoxybenzenesulfonyl)amino-N-(4-chlorocinnamyl)-N-methylbenzylamine) that, at low concentration, is more selective for CaMKII. In contrast, the CaMK kinase inhibitor STO-609 [7H-benzimidazo(2,1-a)benz(de)isoquinoline-7-one-3-carboxylic acid] specifically limits the synthesis and/or availability of PRPs. Analytically powerful three-pathway protocols using sequential strong and weak tetanization in varying orders and test stimulation over long periods of time after LTP induction enable a pharmacological dissociation of these distinct roles of the CaMK pathways in late-LTP and so provide a novel framework for the molecular mechanisms by which synaptic potentiation, and possibly memories, become stabilized

Ricin trafficking in plant and mammalian cells

Abstract: Ricin is a heterodimeric plant protein that is potently toxic to mammalian and many other eukaryotic cells. It is synthesized and stored in the endosperm cells of maturing Ricinus communis seeds (castor beans). The ricin family has two major members, both, lectins, collectively known as Ricinus communis agglutinin ll (ricin) and Ricinus communis agglutinin l (RCA). These proteins are stored in vacuoles within the endosperm cells of mature Ricinus seeds and they are rapidly broken down by hydrolysis during the early stages of post-germinative growth. Both ricin and RCA traffic within the plant cell from their site of synthesis to the storage vacuoles, and when they intoxicate mammalian cells they traffic from outside the cell to their site of action. In this review we will consider both of these trafficking routes

Measurements of Scintillation Efficiency and Pulse-Shape for Low Energy Recoils in Liquid Xenon

Results of observations of low energy nuclear and electron recoil events in liquid xenon scintillator detectors are given. The relative scintillation efficiency for nuclear recoils is 0.22 +/- 0.01 in the recoil energy range 40 keV - 70 keV. Under the assumption of a single dominant decay component to the scintillation pulse-shape the log-normal mean parameter T0 of the maximum likelihood estimator of the decay time constant for 6 keV < Eee < 30 keV nuclear recoil events is equal to 21.0 ns +/- 0.5 ns. It is observed that for electron recoils T0 rises slowly with energy, having a value ~ 30 ns at Eee ~ 15 keV. Electron and nuclear recoil pulse-shapes are found to be well fitted by single exponential functions although some evidence is found for a double exponential form for the nuclear recoil pulse-shape.Comment: 11 pages, including 5 encapsulated postscript figure

Cytosolic chaperones influence the fate of a toxin dislocated from the endoplasmic reticulum

The plant cytotoxin ricin enters target mammalian cells by receptor-mediated endocytosis and undergoes retrograde transport to the endoplasmic reticulum (ER). Here, its catalytic A chain (RTA) is reductively separated from the cell-binding B chain, and free RTA enters the cytosol where it inactivates ribosomes. Cytosolic entry requires unfolding of RTA and dislocation across the ER membrane such that it arrives in the cytosol in a vulnerable, nonnative conformation. Clearly, for such a dislocated toxin to become active, it must avoid degradation and fold to a catalytic conformation. Here, we show that, in vitro, Hsc70 prevents aggregation of heat-treated RTA, and that RTA catalytic activity is recovered after chaperone treatment. A combination of pharmacological inhibition and cochaperone expression reveals that, in vivo, cytosolic RTA is scrutinized sequentially by the Hsc70 and Hsp90 cytosolic chaperone machineries, and that its eventual fate is determined by the balance of activities of cochaperones that regulate Hsc70 and Hsp90 functions. Cytotoxic activity follows Hsc70-mediated escape of RTA from an otherwise destructive pathway facilitated by Hsp90. We demonstrate a role for cytosolic chaperones, proteins typically associated with folding nascent proteins, assembling multimolecular protein complexes and degrading cytosolic and stalled, cotranslocational clients, in a toxin triage, in which both toxin folding and degradation are initiated from chaperone-bound states