Protein fiber linear dichroism for structure determination and kinetics in a low-volume, low-wavelength couette flow cell

High-resolution structure determination of soluble globular proteins relies heavily on x-ray crystallography techniques. Such an approach is often ineffective for investigations into the structure of fibrous proteins as these proteins generally do not crystallize. Thus investigations into fibrous protein structure have relied on less direct methods such as x-ray fiber diffraction and circular dichroism. Ultraviolet linear dichroism has the potential to provide additional information on the structure of such biomolecular systems. However, existing systems are not optimized for the requirements of fibrous proteins. We have designed and built a low-volume (200 μL), low-wavelength (down to 180 nm), low-pathlength (100 μm), high-alignment flow-alignment system (couette) to perform ultraviolet linear dichroism studies on the fibers formed by a range of biomolecules. The apparatus has been tested using a number of proteins for which longer wavelength linear dichroism spectra had already been measured. The new couette cell has also been used to obtain data on two medically important protein fibers, the all-β-sheet amyloid fibers of the Alzheimer's derived protein Aβ and the long-chain assemblies of α1-antitrypsin polymers

Corrosion Behavior of Parylene-Metal-Parylene Thin Films in Saline

In this paper, we study the corrosion behavior of parylene-metal-parylene thin films using accelerated-lifetime soak tests. The samples under test are thin film resistors with a 200 nm layer of Au sandwiched by parylene-C on both sides, fabricated with parylene-metal skin technology. The samples are tested in hot saline both passively and actively, and different failure modes are observed using optical and electron-beam metrologies. Bubbles and delamination are first seen in the samples after 2 days of soaking under passive conditions, and followed by metal corrosion. While under active conditions, either bubbles or parylene breakdowns are observed depending on the thickness of parylene packaging. These results contribute to a better understanding of the failure mechanisms of parylene packaging in body fluids

Micro-volume couette flow sample orientation for absorbance and fluorescence linear dichroism

Linear dichroism (LD) can be used to study the alignment of absorbing chromophores within long molecules. In particular, Couette flow LD has been used to good effect in probing ligand binding to DNA and to fibrous proteins. This technique has been previously limited by large sample requirements. Here we report the design and application of a new micro-volume Couette flow cell that significantly enhances the potential applications of flow LD spectroscopy by reducing the sample requirements for flow linear dichroism to 25 μL (with concentrations such that the absorbance maximum of the sample in a 1-cm pathlength cuvette is not, vert, similar1). The micro-volume Couette cell has also enabled the measurement of fluorescence-detected Couette flow linear dichroism. This new technique enables the orientation of fluorescent ligands to be probed even when their electronic transitions overlap with those of the macromolecule and conversely. The potential of flow-oriented fluorescence dichroism and application of the micro-volume Couette LD cell are illustrated by the collection of data for DNA with minor groove and intercalating ligands: DAPI, Hoechst, and ethidium bromide. As with conventional fluorescence, improved sensitivity compared with absorbance LD is to be expected after instrumentation optimization

Equalization Of QPSK Data Transmission In Specular Multipath

This paper gives performance results for transversal-filter equalization of Quadri phase phase-shift-keyed (QPSK) signals with two-component multipath and demodulator phase error. An analytical expression for optimum, minimum mean-square-error tap weights in terms of the multipath and signal parameters is given. Probability of error results for no equalization and equalization with adaptive decision-feedback tap-weight adjustment are compared. The results show that significant improvement can be obtained with relatively simple equalizer structures. Copyright © 1974 by The Institute of Electrical and Electronics Engineers, Inc

Evidence for a Z < 8 Origin of the Source Subtracted Near Infrared Background

This letter extends our previous fluctuation analysis of the near infrared background at 1.6 microns to the 1.1 micron (F110W) image of the Hubble Ultra Deep field. When all detectable sources are removed the ratio of fluctuation power in the two images is consistent with the ratio expected for faint, z<8, sources, and is inconsistent with the expected ratio for galaxies with z>8. We also use numerically redshifted model galaxy spectral energy distributions for 50 and 10 million year old galaxies to predict the expected fluctuation power at 3.6 microns and 4.5 microns to compare with recent Spitzer observations. The predicted fluctuation power for galaxies at z = 0-12 matches the observed Spitzer fluctuation power while the predicted power for z>13 galaxies is much higher than the observed values. As was found in the 1.6 micron (F160W) analysis the fluctuation power in the source subtracted F110W image is two orders of magnitude below the power in the image with all sources present. This leads to the conclusion that the 0.8--1.8 micron near infrared background is due to resolved galaxies in the redshift range z<8, with the majority of power in the redshift range of 0.5--1.5.Comment: Accepted for publication in the Astrophysical Journa

Hybrid Carrier And Modulation Tracking Loop Performance In RFI

The operation of a hybrid Tracking (HT) phase-locked loop (PLL) in radio-frequency interference (RFI) is considered. Results from a perturbation analysis for the loop phase error in continuous-wave (CW) RFI are found to compare favorably with results obtained by digital computer simulation. These results, together with simulation results for wide-band (in comparison to the loop bandwidth) RFI, indicate that loop structures which track both on the signal carrier and modulation components are advantageous in RFI plus Gaussian noise backgrounds. Copyright © 1974 by The Institute of Electrical and Electronics Engineers, Inc

From Kiobel Back to Structural Reform: The Hidden Legacy of Holocaust Restitution Litigation

This paper offers a new approach to the issue of transnational corporate liability for human rights violations and more generally an inquiry into the place of domestic legal experiences in theorizing about transnational law. Grounded in a study of the Holocaust restitution litigation of the 1990s, we explain corporate liability as a type of bureaucratic liability and explore in depth the relationship between the Holocaust litigation and the theory of structural reform litigation developed in the U.S. to address the bureaucratic structure of rights violations. We read the restitution litigation in light of pluralist reformulations of structural reform, in which norms are not enunciated in a hierarchical manner by the judge but are produced contextually through dialogue between court and parties. We use this analysis to challenge contemporary theoretical treatments of transnational corporate responsibility for human rights violations and suggest more promising directions for theorization