Forecasting Methodologies for USAF Facility Maintenance and Repair Funding Requirements

Eighteen methodologies for forecasting facility maintenance and repair funding requirements were investigated and analyzed to determine which methodology is best suited for use by the United States Air Force (USAF). The literature review identified four primary factors, or criteria, that determine facility maintenance and repair funding requirements. The methodologies were scored against the four criteria with respect to their appropriate application to USAF requirements. An analysis of dominance was accomplished; the results suggested that no one methodology was clearly superior. Fourteen of the methodologies were dominated, and consequently eliminated from further analysis. Four methodologies were non-dominated: the U.S. Army Construction Engineering Research Laboratories (USACERL) BUILDER; USACERL Maintenance Resource Prediction Model; U.S. Army Installation Status Report; and the USAF Plant Replacement Value-Facility Investment Metric (PRV-FIM). Further analysis was accomplished using the multi-criteria decision-making techniques of lexicographic analysis and the Technique for Order Preference by Similarity to Ideal Solution (TOPS IS). The results suggested the USAF PRV-FIM methodology is only preferable when the most important consideration is limiting the amount of data that must he collected and maintained. Otherwise, the USACERL BUILDER methodology may best serve the USAF in justifying to Congress and the public, its facility maintenance and repair level of investment determination

Single nucleotide polymorphism discovery in elite north american potato germplasm

BACKGROUND: Current breeding approaches in potato rely almost entirely on phenotypic evaluations; molecular markers, with the exception of a few linked to disease resistance traits, are not widely used. Large-scale sequence datasets generated primarily through Sanger Expressed Sequence Tag projects are available from a limited number of potato cultivars and access to next generation sequencing technologies permits rapid generation of sequence data for additional cultivars. When coupled with the advent of high throughput genotyping methods, an opportunity now exists for potato breeders to incorporate considerably more genotypic data into their decision-making. RESULTS: To identify a large number of Single Nucleotide Polymorphisms (SNPs) in elite potato germplasm, we sequenced normalized cDNA prepared from three commercial potato cultivars: 'Atlantic', 'Premier Russet' and 'Snowden'. For each cultivar, we generated 2 Gb of sequence which was assembled into a representative transcriptome of (~)28-29 Mb for each cultivar. Using the Maq SNP filter that filters read depth, density, and quality, 575,340 SNPs were identified within these three cultivars. In parallel, 2,358 SNPs were identified within existing Sanger sequences for three additional cultivars, 'Bintje', 'Kennebec', and 'Shepody'. Using a stringent set of filters in conjunction with the potato reference genome, we identified 69,011 high confidence SNPs from these six cultivars for use in genotyping with the Infinium platform. Ninety-six of these SNPs were used with a BeadXpress assay to assess allelic diversity in a germplasm panel of 248 lines; 82 of the SNPs proved sufficiently informative for subsequent analyses. Within diverse North American germplasm, the chip processing market class was most distinct, clearly separated from all other market classes. The round white and russet market classes both include fresh market and processing cultivars. Nevertheless, the russet and round white market classes are more distant from each other than processing are from fresh market types within these two groups. CONCLUSIONS: The genotype data generated in this study, albeit limited in number, has revealed distinct relationships among the market classes of potato. The SNPs identified in this study will enable high-throughput genotyping of germplasm and populations, which in turn will enable more efficient marker-assisted breeding efforts in potato

The Turkish history, from the original of that nation, to the growth of the Ottoman empire [electronic resource] : with the lives and conquests of their princes and emperours /

Volume 1 port signed: "P. Lelij Pinxit ; R. White, Sculpsit."Vol. 2 has title: "The Turkish history. The second volume, beginning from Mahomet III and continued to this present year 1687. The sixth edition." has separate dated title page and register.Irregular pagination.Wing (2nd ed., 1994),ESTCVol. 2 includes: "A continuation of this present history ... by Edward Grimston", "Continuation of the Turkish history ...collected out of the papers and dispatches of Sir Thomas Roe", "The history of the Turkish empire continued, by Sir Roger Manley", and "The present state of the Ottoman empire, by Paul Rycaut."Vol. 1 includes advertisements on p. [27].Includes indices.Imperfect: lacks pages 263-276 of v. 2 ; print show-through with slight loss of text.Reproduction of original in: York Minster. Library.Electronic reproduction