45 research outputs found
ZBP-89 function in colonic stem cells and during butyrate-induced senescence
ZBP-89 (Zfp148, ZNF148) is a Kruppel-type zinc-finger family transcription factor that binds to GC-rich DNA elements. Earlier studies in cell lines demonstrated that ZBP- 89 cooperates with Wnt β-catenin signaling by inducing β-catenin gene expression. Since β-catenin levels are normally highest at the crypt base, we examined whether ZBP-89 is required for stem cell maintenance. Lineage-tracing using a Zfp148Cre transgenic line demonstrated expression in both intestine and colonic stem cells. Deleting the Zfp148 locus in the colon using the Cdx2NLSCre transgene, reduced the size and number of polyps formed in the Apc-deleted mice. Since colon polyps form in the presence of butyrate, a short chain fatty acid that suppresses cell growth, we examined the direct effect of butyrate on colon organoid survival. Butyrate induced senescence of colon organoids carrying the Apc deletion, only when Zfp148 was deleted. Using quantitative PCR and chromatin immunoprecipitation, we determined that butyrate treatment of colon cell lines suppressed ZNF148 gene expression, inducing CDKN2a (p16 ) gene expression. Collectively, Zfp148 mRNA is expressed in CBCs, and is required for stem cell maintenance and colonic transformation. Butyrate induces colonic cell senescence in part through suppression of ZBP-89 gene expression and its subsequent occupancy of the CDKN2A promoter. ERT2 ERT2 Ink4ahttp://deepblue.lib.umich.edu/bitstream/2027.42/168213/2/ZBP-89 function in colonic stem cells and during butyrate-induced senescence.pdfPublished versionDescription of ZBP-89 function in colonic stem cells and during butyrate-induced senescence.pdf : Published versio
Investigating porcine parvoviruses genogroup 2 infection using in situ polymerase chain reaction
Abstract Background Porcine parvovirus 2 (PPV2) was detected in swine serum without showing any relationship with disease. The emergence of the virus seemed to be a unique event until other genetically highly similar parvoviruses were identified in China and, later in 2012, the presence of the virus was also described in Europe. PPV2 is widely distributed in pig populations where it is suspected to be involved in respiratory conditions, based on its frequent detection in lung samples. In order to investigate the potential pathogenic involvement of PPV2, 60 dead pigs were examined from two farms. They were necropsied and tested for PPV2 and PCV2 (Porcine circovirus type 2) by PCR; by Brown and Brenn (B&B) staining for bacteria; by immunohistochemistry (IHC) to detect CD3, Swine leukocyte antigen class II DQ (SLAIIDQ), lysozyme, porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza (SIV), Mycoplasma hyopneumoniae (Mhyo); and by in situ hybridization (ISH) to detect ssDNA and dsDNA of PCV2. PPV2 positive samples were subjected to in situ polymerase chain reaction (IS-PCR) including double staining method to detect PPV2 and host cell markers. To calculate statistical difference we used GENMOD or LOGISTIC procedures in Statistical Analysis System (SASÂŽ). Results We found that the PPV2 was localized mostly in lymphocytes in lungs, lymph nodes and liver. Neither CD3 antigen nor lysozyme was expressed by these infected cells. In contrast, low levels of SLAIIDQ were expressed by infected cells, suggesting that PPV2 may have a specific tropism for immature B lymphocytes and/or NK lymphocytes though possibly not T lymphocytes. Conclusion The overall conclusion of this study indicates that PPV2 may contribute to the pathogenesis of pneumonia
Recent Innovations & Daily Problems. A new prosthesis in inguinal hernia repair:preliminary results of a pilot study.
Introduction: Elective surgery for inguinal hernia is affected by very
low mortality ÂŤ 1 per 10000 operation); in contrast, when surgery
is carried out for complicated inguinal hernia, risks of postoperative
complication are higher. TAPP is a world-wide accepted surgical practice
in the treatment of elective bilateral or recurrent inguinal hernia,
above all in young patients. Few exploratory studies were published on
laparoscopic approach in the treatment of urgent complicated inguinal
hernia. Aim of this study was to analyze feasibility (operative time,
conversion rate), safety (postoperative morbidity, length of hospital
stay) and quality of life (acute and chronic pain, return to work) of
trans-abdominal pre-peritoneal laparoscopic hernia repair in acute
incarcerated inguinal hernia. Rationale of laparoscopic trans-abdominal
approach is the easier hernia reduction under vision and a better
exploration of the abdominal cavity.
Methods: from September 2012 to September 2013, 15 consecutive
patients admitted in emergency at the Division of General Surgery of
University "Sapienza", Polo Pontino, for acute incarcerated inguinal
hernia were submitted to TAPP using 3 trocars (1 of 10 mm and 2 of
5mm) and polyester prosthesis fixed by fibrin glue. Exclusion criteria
for laparoscopic approach were age III, previous
abdominal surgery, signs of strangulated hernia. All of them were
evaluated for operative time, conversion rate, postoperative morbidity,
organ resection or other surgery required. All patients were scored for
pain by Visual Analogic Scale (VAS) during postoperative in hospital
stay at 7 days, 1,6 and 12 months after surgery.
Results: median follow-up was 16 months and 12 as minimum. In all
cases reduction of hernia was always possible and none conversion
to open surgery was recorded, median operative time was 89 minutes
(55-137 as range), omental resection was carried out in one patient
(6,6%), no other organ resections needed, whereas contralateral hernia
was diagnosed and repaired at the same time in 4 patients (26,6%). No
major complications were observed, median blood loss was 100 ml,
minor morbidity was contained to 18% represented by fever and
wound infection of surgical umbilical scar. Median in hospital stay
was 1,5 days with 1-5 days as range. Postoperative median acute pain,
measured by visual analogic scale (VAS), was 2 (range:0-4), none
patient referred any pain during follow-up. Median time of return to
work was 6,5 days, ranged between 3 to 15 days. Patients' compliance
to treatment and to follow-up was complete as well their satisfaction. Conclusions: In centres skilled for laparoscopy in emergency, TAPP
could be considered a feasible and safe technique. In well-selected
patients (especially if emolled in controlled clinical trial) TAPP could
represent an alternative surgical approach for complicated incarcerated inguinal hernia to conventional open surgery even in urgency. The
main advantages of laparoscopic approach are the ability to perform
surgical hernia reduction under vision, a better exploration and evaluation
of abdominal cavity and diagnosis and treatment of eventual
contralateral defect of wall, otherwise often missed. Finally, the good
control of acute and chronic pain, faster return to normal activity and
work, better aesthetic results contributed to total satisfaction and compliance
of the patients
Thermodynamic Description of the ACl-ThCl4 (A = Li, Na, K) Systems
The ACl-ThCl4 (A = Li, Na, K) systems could be of relevance to the nuclear industry in the near future. A thermodynamic investigation of the three binary systems is presented herein. The excess Gibbs energy of the liquid solutions is described using the quasi-chemical formalism in the quadruplet approximation. The phase diagram optimisations are based on the experimental data available in the literature. The thermodynamic stability of the liquid solutions increases in the orderLi Ä Na Ä K, in agreement with idealised interactions and structural model
Correction: OcĂĄdiz Flores et al. Thermodynamic Description of the ACl-ThCl4 (A = Li, Na, K) Systems
Corrected excess Gibbs energies of the liquid solutions in the ACl-ThCl4 (A = Li, Na, K), as well as revised standard enthalpies of formation and standard entropies of the intermediate phases occurring in the binary systems, are presented. The phase diagrams are reproduced to a similar level of accuracy as in the original publication, and the trends in thermodynamic stability of the liquid solutions are maintained. That is, the main conclusions of the paper are not affected. The original publication has also been update
Using the Quasi-chemical formalism beyond the phase Diagram: Density and viscosity models for molten salt fuel systems
CALPHAD models to compute the density and viscosity of four keystone systems related to Molten Salt Reactor (MSR) technology have been optimized: NaCl-UCl3, LiF-ThF4, LiF-UF4, and LiF-ThF4-UF4. Revised thermodynamic assessments of all four systems, using the modified quasichemical formalism in the quadruplet approximation for the description of the liquid solutions, are reported. In the case of NaCl-UCl3, phase diagram and mixing enthalpy data available in the literature are taken into account. For the fluoride systems, recently published data on some solid phases are taken into account, while retaining the most recently published descriptions of the liquid solutions. The densities of the liquid solutions are modelled using pressure-dependent terms of the excess Gibbs energy, while the viscosities are then modelled using an Eyring equation. Both state functions are related to the thermodynamic assessments through the quadruplet distributions.RST/Reactor Physics and Nuclear Material
Thermodynamic Description of the ACl-ThCl4 (A = Li, Na, K) Systems
The ACl-ThCl4 (A = Li, Na, K) systems could be of relevance to the nuclear industry in the near future. A thermodynamic investigation of the three binary systems is presented herein. The excess Gibbs energy of the liquid solutions is described using the quasi-chemical formalism in the quadruplet approximation. The phase diagram optimisations are based on the experimental data available in the literature. The thermodynamic stability of the liquid solutions increases in the orderLi Ä Na Ä K, in agreement with idealised interactions and structural modelsRST/Reactor Physics and Nuclear Material
A promising fuel for fast neutron spectrum Molten Salt Reactor: NaCl-ThCl<sub>4</sub>-PuCl<sub>3</sub>
Chloride salts are considered a good alternative to fluoride salts as fuel carrier in the Molten Salt Fast Reactor concepts. The NaClâThCl4âPuCl3 fuel salt solution seems very promising, with low melting temperature eutectic compositions, and the potential to be used in a breeder and burner type of reactor design. This work focuses on the first thermodynamic modeling assessment of the ThCl4âPuCl3 binary system and the NaClâThCl4âPuCl3 ternary system, using the CALPHAD (Computer Coupling of Phase Diagrams and Thermochemistry) method and the quasichemical formalism in the quadruplet approximation. The investigated system shows potential for a high flexibility with respect to composition at operating temperatures, which can be beneficial to accommodate the requirements on other essential fuel properties (e.g. neutronic and thermo-hydraulic).RST/Reactor Physics and Nuclear Material
Novel câMYC target genes mediate differential effects on cell proliferation and migration
Excreted/secreted Schistosoma mansoni venom allergen-like 9 (SmVAL9) modulates host extracellular matrix remodelling gene expression
Host-parasite interactio