Potential drug target from breast milk Lactobacillus against vaginal pathogens

The term “Probiotics” refers to the micro-organisms that confers health benefits to hosts when administered in adequate amounts. In this work, Lactobacillus was isolated from breast milk of  a 26 yr old women and was treated against vaginal pathogens by varying in different concentration (50µl, 40µl and 30µl). Identification of Lactobacillus was carried out by motility, gram staining and biochemical test. The antibacterial effects of the Lactobacillus against vaginal pathogens were carried out by disc Agar diffusion method and Antibiotic sensitivity test was also analyzed for the pathogens. The antimicrobial activity of the sample revealed that the Lactobacillus isolated from breast milk showed significant effectively against vaginal pathogens especially higher for Klebsiella pneumonia. GC-MS was carried out to identify bioactive compounds, followed by the identification of novel bioactive compounds in the corresponding fraction. The main aim is to assess the probiotic nature of Lactobacillus in preventing cervical pathogens by studying the effectiveness of antimicrobial activity against vaginal pathogens by identifying the effective compounds by GC-MS and they may widened up the panorama in research and may act as a promising natural human source based drug in medical field without taking any chemical drugs which cause side effects