Influencing the secretion of myogenic factors from mesenchymal stem cells.

Mounting evidence indicates that the regenerative effect of mesenchymal stem cells in skeletal muscle is related to the secretion of factors that stimulate resident myogenic cells. However, the environmental cues that affect the secreted factors of mesenchymal stem cells are not well understood. A recent publication demonstrated that secretion of factors is dependent on cell substrate, with mesenchymal stem cells grown on laminin providing more pro-myogenic factors than those grown on collagen, and that cellular strain may also play a role. Conditioned media from mesenchymal stem cells grown on laminin and subjected to strain provided the quickest and largest stimulation to myogenic cell proliferation. The influence of cell substrate and mechanical perturbation on mesenchymal stem cells therefore appears key to secretion of factors that support myogenesis

Spatiotemporal dynamics in 2D Kolmogorov flow over large domains

Kolmogorov flow in two dimensions - the two-dimensional Navier-Stokes equations with a sinusoidal body force - is considered over extended periodic domains to reveal localised spatiotemporal complexity. The flow response mimicks the forcing at small forcing amplitudes but beyond a critical value develops a long wavelength instability. The ensuing state is described by a Cahn-Hilliard-type equation and as a result coarsening dynamics are observed for random initial data. After further bifurcations, this regime gives way to multiple attractors, some of which possess spatially-localised time dependence. Co-existence of such attractors in a large domain gives rise to interesting collisional dynamics which is captured by a system of 5 (1-space and 1-time) PDEs based on a long wavelength limit. The coarsening regime reinstates itself at yet higher forcing amplitudes in the sense that only longest-wavelength solutions remain attractors. Eventually, there is one global longest-wavelength attractor which possesses two localised chaotic regions - a kink and antikink - which connect two steady one-dimensional flow regions of essentially half the domain width each. The wealth of spatiotemporal complexity uncovered presents a bountiful arena in which to study the existence of simple invariant localised solutions which presumably underpin all of the observed behaviour

Identifying Health Centers in Honduras Infested with Rhodnius Prolixus Using the Seroprevalence of Chagas Disease in Children Younger than 13 Years.

The objective of this study is to determine if a Chagas disease protocol starting with a serological survey is as reliable at identifying insect-infested areas as one using the gold standard entomological survey. The study found that health center areas infested with Rhodnius prolixus were identified using a threshold seroprevalence of 0.1%. The serological survey took half the time and was 30% less expensive than the entomological survey. Developing countries with limited resources may find this strategy useful in combating Chagas disease. This strategy also identifies seropositive children, which facilitates their treatment

Explaining global increases in water use efficiency: why have we overestimated responses to rising atmospheric CO(2) in natural forest ecosystems?

BackgroundThe analysis of tree-ring carbon isotope composition (δ(13)C) has been widely used to estimate spatio-temporal variations in intrinsic water use efficiency (iWUE) of tree species. Numerous studies have reported widespread increases in iWUE coinciding with rising atmospheric CO(2) over the past century. While this could represent a coherent global response, the fact that increases of similar magnitude were observed across biomes with no apparent effect on tree growth raises the question of whether iWUE calculations reflect actual physiological responses to elevated CO(2) levels.Methodology/resultsHere we use Monte Carlo simulations to test if an artifact of calculation could explain observed increases in iWUE. We show that highly significant positive relationships between iWUE and CO(2) occur even when simulated data (randomized δ(13)C values spanning the observed range) are used in place of actual tree-ring δ(13)C measurements. From simulated data sets we calculated non-physiological changes in iWUE from 1900 to present and across a 4000 m altitudinal range. This generated results strikingly similar to those reported in recent studies encompassing 22 species from tropical, subtropical, temperate, boreal and mediterranean ecosystems. Only 6 of 49 surveyed case studies showed increases in iWUE significantly higher than predicted from random values.Conclusions/significanceOur results reveal that increases in iWUE estimated from tree-ring δ(13)C occur independently of changes in (13)C discrimination that characterize physiological responses to elevated CO(2). Due to a correlation with CO(2) concentration, which is used as an independent factor in the iWUE calculation, any tree-ring δ(13)C data set would inevitably generate increasing iWUE over time. Therefore, although consistent, previously reported trends in iWUE do not necessarily reflect a coherent global response to rising atmospheric CO(2). We discuss the significance of these findings and suggest ways to distinguish real from artificial responses in future studies

Boost the Impact of Continuous Formal Verification in Industry

Software model checking has experienced significant progress in the last two decades, however, one of its major bottlenecks for practical applications remains its scalability and adaptability. Here, we describe an approach to integrate software model checking techniques into the DevOps culture by exploiting practices such as continuous integration and regression tests. In particular, our proposed approach looks at the modifications to the software system since its last verification, and submits them to a continuous formal verification process, guided by a set of regression test cases. Our vision is to focus on the developer in order to integrate formal verification techniques into the developer workflow by using their main software development methodologies and tools.Comment: 7 page