Effect of temperature and relative-humidity on the development of Liriomyza sativae Blanchard (Diptera: Agromyzidae) in Vigna unguiculata

A pesquisa teve como objetivo estudar a influência da temperatura e da umidade relativa do ar (UR) no desenvolvimento de Liriomyza sativae Blanchard, durante o período ovo-adulto, em feijão caupi, para fornecer subsídios a futuros projetos de controle biológico da praga. Verificou-se uma relação inversa entre o aumento da temperatura na faixa de 15°C a 32°C e a duração do desenvolvimento. A sobrevivência larval não foi afetada na faixa térmica estudada, enquanto a 32°C houve alta mortalidade de pupas (59,9%). A UR não afetou a duração dos estágios imaturos, embora tenha influenciado a sua sobrevivência. O limiar térmico inferior de desenvolvimento obtido para o período ovo-adulto foi baixo (7,3°C), se comparado a outras espécies de Liriomyza, e bastante reduzido para a fase larval (3,4°C). De acordo com as exigências térmicas constatadas para L. sativae foi possível estimar a ocorrência de 24,5 gerações anuais na região produtora de melão, em Mossoró, RN. Para criações de laboratório, visando à implementação de programas de controle biológico da praga, as melhores condições são temperatura de 30°C e UR de 50% para a fase de larva e 90% para o estágio de pupa de L. sativae.This research aimed to study the influence of temperature and relative-humidity (RH) on the development of Liriomyza sativae Blanchard during the egg-adult period, in cowpea, to provide essential information for future biological control projects against the pest. An inverse relation was observed between temperature increase in the range from 15°C to 32°C and development duration. Larval survival was not affected in the temperature range studied, while a high mortality of pupae was observed at 32°C (59.9%). RH did not affect the development time of the immature stages, although it influenced their survival. The lower developmental temperature threshold obtained for the egg-adult period was low (7.3°C) when compared with other species of Liriomyza, and was rather low for the larval stage (3.4°C). Based on the thermal requirements for L. sativae, it was possible to estimate the occurrence of 24.5 annual generations at a melon producing region in state of Rio Grande do Norte, Brazil. For laboratory rearing aimed at biological control pest programs, the best rearing conditions are 30°C and 50% RH for the larval stage and 90% RH for the pupal stage.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

BioAssay: a long road through the years toward open science.

Since the publication of the first issue in 2006, BioAssay has been publishing original and current articles on the evaluation of the biological activity of arthropod control agents of agricultural, forestry, urban, veterinary and medical importance. Throughout these years, BioAssay published 83 articles in six different sections (Chemical Control, Plant Extracts, Biological Control, Scientific Notes, Semiochemicals, and Forum). As of 2023, we are starting a new era, aimed at adapting the journal to Open Science

Sistema de criação de parasitoide de mosca-minadora.

Este trabalho apresenta o passo a passo do sistema de criação de parasitoides larva-pupa de moscas-minadoras. Como modelo, foi utilizadou o braconídeo, O. scabriventris, multiplicado sobre a mosca-minadora, L. sativae.bitstream/item/165686/1/CTE116.pd

Determination of Moisture Levels, Protein and Water Absorption of Chicken Giblets

Abstract This study aimed at evaluating the levels of moisture, protein, water to protein ratio, and water absorption during chilling of chicken giblets (heart, liver, and gizzard) to set legal limits of water absorption during this process. The survey was conducted in the southern Brazil, the largest broiler-producing region of this country. Giblets (heart, liver, and gizzard) were collected fresh from the processing line after evisceration and at the exit of the chiller after the immersion process from two processing plants. One of the plants (PP1) processes small chickens (1,100g live weight) and PP2 processes chickens with 2,800g live weight. In total, 448 samples were collected. Laboratory tests were performed in duplicate for each parameter measured. The results show that moisture levels of fresh giblets were higher in the gizzard, followed by the liver and the heart, whereas in chilled giblets, the gizzard still maintained the highest moisture level, but was followed by the heart and then the liver. Both in fresh and chilled samples, the liver presented the highest protein content, followed by the gizzard and the heart. Water to protein ratios were higher in chilled than in fresh samples, and was highest in the heart, followed by the gizzard and the liver. After immersion in the chiller, the heart presented the highest water absorption rate (6.59%), which was significantly higher compared with those of the liver (4.16%) and the gizzard (4.51%). Considering that the water absorption rates obtained both in fresh and chilled chicken giblets were below 8.00%, the following upper limits of water absorption are suggested for chicken giblet processing in Brazil: 7.0% for the heart, and 5.0% for the gizzard and the liver

Reproductive Activity and Survivorship of Liriomyza sativae (Diptera: Agromyzidae) at Different Temperatures and Relative Humidity Levels

We studied a population of Liriomyza sativae Blanchard (Diptera: Agromyzidae) identified by morphological and molecular techniques from the semiarid region of the Brazilian northeast. The influence of temperature and relative humidity on the survival and reproductive parameters of L. sativae in cowpeas (Vigna unguiculata L. Walp.) (Fabales: Fabaceae) was evaluated. We used temperatures of 18, 20, 22, 25, 28, 30, and 32 +/- 1 degrees C (50 +/- 10% RH) and relative humidity values of 30,50,70, and 90 +/- 10% (25 +/- 1 degrees C) under a 14 L:10 D photoperiod. Adult longevity decreased as temperature and relative humidity increased and was greater, in general, for females. The preoviposition and oviposition periods also decreased as temperature increased, whereas relative humidity only caused reductions in the oviposition period at higher levels. Fecundity was similar in the range from 18 to 30 degrees C but decreased at 32 degrees C with respect to relative humidity; the best performances of L. sativae occurred at lower levels. The pattern of oviposition rate changed with temperature and relative humidity. Regardless of temperature and relative humidity, L, sativae laid between 75 and 92% of its eggs on the adaxial surface of the cowpea leaves. This information will be highly useful to design a leafminer production system aimed at the multiplication of natural enemies, as well as for pest management in the field.CAPES Coordenacao de Aperfeicoamento de Nivel Superio

240 First year experience of hereditary testing in gynecological cancer patients in a clinical setting in the bahamas

ObjectivesIn a previous cohort of predominantly breast cancer patients, ∼25% of cases had 7 recurrent mutations in BRCA1 and BRCA2.The rate of hereditary ovarian cancer in the Bahamas was sought using an Oncology-led point of genetic testing at the Princes Margaret Hospital (PMH) in the Bahamas.MethodsWomen were counseled and consented for genetic testing in the Gynecology Oncology clinic at our facility. Saliva samples underwent next generation sequencing (NGS) in a CLIA approved external laboratory. A 30 gene panel linked to breast, ovarian and/or uterine cancer risks was used to identify: BRCA1, BRCA2, Lynch genes, MLH1, MSH2, MSH6, PMS2, EPCAM, MUTYH, APC, STK11, PALB2, MITF, BAP1, CDKN2A, TP53, BMPR1A, SMAD4, POLD1, POLE1, CHEK2, PTEN, CDH1, BRIP1, CDK4, GREM1, RAD51C, RAD51D, PMS2, NBNand BARD1. Reportsincluded presence or absence of deleterious mutations and variants of unknown significance (VUS).ResultsBetween 03/2018–03/2019, 28 women were tested, 17 women had ovarian cancer, 7 endometrial cancer, 2 breast cancer, and 2 women with a strong family history of ovarian cancer. The mean age at testing was 60 years. 21.4% had a deleterious mutation in BRCA: 5 in BRCA1 and 1 in BRCA2. Of the patients with BRCA mutation 5/6 women were diagnosed with ovarian cancer (29.5%) and 1/6 had ductal carcinoma of the breast.ConclusionsGenetic testing at point of care in the Bahamas is feasible and acceptable. Results highlight need for universal hereditary screening for women in the Bahamas with ovarian cancer as this can allow for better treatment options