New Phosphated Poly(methyl Methacrylate) Polymers for the Prevention of Denture-induced Microbial Infection: an In Vitro Study

Purpose: Poly(methyl methacrylate) (PMMA) has been widely used as a denture-base acrylic resin due to its excellent physical and mechanical properties. However, the material is highly prone to microbial fouling that often leads to Candida-associated denture stomatitis. Incorporation of phosphate groups into PMMA could facilitate adsorption of salivary antimicrobials and inhibit microbial adherence on the polymer surface. An in vitro study evaluated PMMA polymers containing varying amounts of phosphate group for their efficacy to inhibit Candida albicans adhesion, adsorb salivary histatin 5, and exhibit candidacidal activity. Methods: Six PMMA polymers containing 0%, 5%, 15%, 10%, 20%, and 25% of phosphate group were synthesized by bead (suspension) polymerization technique using mixtures of methyl methacrylate and methallyl phosphate as monomers. The efficacy of the polymers to inhibit the adherence of C. albicans was examined by using human saliva-coated polymer beads and radio-labeled C. albicans cells, as compared with that of PMMA. The potency of the phosphated PMMA polymers to adsorb histatin 5 was determined by measuring the radioactivity of the adsorbed labeled-peptide on the polymer surface. The candidacidal activity of the histatin 5-adsorbed polymers was assessed by using the fluorescence technique. The percent release of the fluorescent probe calcein from the C. albicans membrane caused by the disruption of the cell membrane was determined. The data were analyzed statistically by one-way ANOVA followed by Scheffé’s test (α = 0.05 and n = 6). Results: The presence of ≥15% phosphate content in PMMA significantly reduced the saliva-mediated adhesion of C. albicans. Phosphated PMMA polymers showed significantly enhanced adsorption of histatin 5 in a phosphate density-dependent manner. The candidacidal activity of the histatin 5-bound polymers increased significantly with the increase in the phosphate content of the polymer. Conclusion: Phosphated PMMA polymers have the potential to serve as novel denture-base resins, which may reduce C. albicans colonization and prevent denture stomatitis

Spatially extended Unruh-DeWitt detectors for relativistic quantum information

Unruh-DeWitt detectors interacting locally with a quantum field are systems under consideration for relativistic quantum information processing. In most works, the detectors are assumed to be point-like and, therefore, couple with the same strength to all modes of the field spectrum. We propose the use of a more realistic detector model where the detector has a finite size conveniently tailored by a spatial profile. We design a spatial profile such that the detector, when inertial, naturally couples to a peaked distribution of Minkowski modes. In the uniformly accelerated case, the detector couples to a peaked distribution of Rindler modes. Such distributions are of special interest in the analysis of entanglement in non-inetial frames. We use our detector model to show the noise detected in the Minkowski vacuum and in single particle states is a function of the detector's acceleration.Comment: Revised for publication, 9 pages (+1 references page), 7 figure

Linear effects of perturbed recombination

Perturbations in the ionization fraction after recombination affect the Compton cooling of density perturbations. Once the gas temperature starts to decouple from the CMB temperature, ionization fraction perturbations can have a significant influence on the subsequent gas temperature perturbation evolution. This directly affects the 21cm spin temperature of the gas, and also modifies the small-scale baryon perturbation evolution via the difference in baryon pressure. The effect on the gas temperature perturbations can be significant on all scales, and galactic-scale baryon perturbations are modified at the percent level at redshifts z >~ 100 where numerical simulations are typically started.Comment: 5 pages; for more details of effect on 21cm see astro-ph/0702600; code available at http://camb.info/sources

Analysis of the flowability of cohesive powders using Distinct Element Method

Computer simulations using Distinct Element Method (DEM) have been carried out to investigate the effect of cohesion on the flowability of polydisperse particulate systems. For this purpose, two assemblies with different values of surface energy and made of 3000 spheres with the mechanical properties of glass beads were considered. The analysis of the flowability of the powders is presented in terms of the unconfined yield stress as a function of strain rate for different pre-consolidation loads. For values of the surface energy of 1.0 J/m2 and strain rates lower than 6 s− 1, the unconfined yield stress does not change significantly indicating a quasi-static behaviour of the particulate assemblies during the compression process. For larger strain rates, the unconfined yield stress varies with the power index of 1.2 of the strain rate. The influence of the pre-consolidating stress on the powder behaviour has also been investigated and a flow factor was obtained from the linear relationship between the unconfined yield stress and pre-consolidation stress. The computer simulations show qualitatively a good agreement with the experimental trends on highly cohesive powder flow behaviour