Innovative technologies of grain sorghum processing

© Published under licence by IOP Publishing Ltd. The article presents materials for the production of grain sorghum food products that allows you to completely eliminate from the diet foods containing gluten, vegetable oil from germ of grain sorghum, nanocellulose, tannin, amylopectinosis sorghum starch and glucose-fructose syrup

Situational problems as a means for forming the professional competencies of university students

© 2015 by the author(s). The purpose of this article aims to study the problem of forming students' competencies during the professional training in the university. The paper demonstrates a methodology for the development and application of situational problems as an effective means of forming the professional competencies of university students. The basis of the presented work is the ideas of the competence-based and the activity-based approaches, expert evaluation of the university students' educational activity. The article describes the technology for developing the situational problems, based on the analysis of the professional roles. This article is intended for educators, researchers, and heads of educational institutions that are into the formation and diagnostics of university students' professional competencies

Impact of Transphyseal Elastic Nailing On the Histostructure of the Tibia in Growing Animals (Non-Randomized Controlled Experimental Study)

BACKGROUND: The use of intramedullary elastic nailing is a method of choice for prevention of complications in children with osteogenesis imperfecta. However, the morphology of the growing long bones in the conditions created was not investigated. AIM: The purpose of our experiment was to study the impact of elastic intramedullary nailing on the histostructure of long bones in their physiological growth. METHODS: Six mongrel dogs underwent intramedullary elastic transphyseal nailing of the intact tibia with two titanium wires. Six months after nailing, a light-optical microscopic and histomorphometric study of the operated and contralateral tibiae was performed. RESULTS: It was found that asymmetric lesion of the distal physis induces a decrease in the height of the distal epimetaphysis. Adaptive changes in the hyaline cartilage of both articular ends were revealed corresponding to the initial stage of chondropathy. Intramedullary nailing promotes an increase in the thickness of the compact bone and the volume of the trabecular bone. CONCLUSIONS: Elastic transphyseal nailing of the intact tibia has a shaping effect which is expressed by an increase in the volume of spongy and compact bone, adaptive changes in the hyaline cartilage. Asymmetric damage to growth zones should be avoided to prevent deformities

Innovative technologies of grain sorghum processing

© Published under licence by IOP Publishing Ltd. The article presents materials for the production of grain sorghum food products that allows you to completely eliminate from the diet foods containing gluten, vegetable oil from germ of grain sorghum, nanocellulose, tannin, amylopectinosis sorghum starch and glucose-fructose syrup

Orthopaedic and somatic status in patients with osteogenesis imperfecta

Introduction Osteogenesis imperfecta (OI) is a group of rare and relatively diverse genetic disorders, characterized by frequent fractures, bone deformities, low bone mineral density and osteopenia. Frequent fractures, bone deformities, and nonunion are among orthopedic problems in OI patients. Varus deformity of the femoral neck, scoliosis, protrusion of the acetabulum, static deformities of the feet are considered separately. The main goal of surgical treatment of the limb deformities and fractures in OI patients is restoration and maintenance of their motor activity, autonomy and preservation of quality of life in severe OI types. The purpose of this study was to assess the deformities in the lower extremities and other accompanying orthopedic disorders, as well as the somatic status of patients with severe OI types (Sillence’s types III and IV) at the point of admission to operative orthopedic treatment. Material and methods Between 2003 and 2016, we examined 43 patients with severe OI (types III and IV) which were admitted for operative correction of deformities of the lower and upper extremities, nonunion, and varus deformity of the femoral neck. The average age at the time of admission was 14.4 ± 9.74 years (from 2 years 9 months to 46 years). Results Treatment of fractures was conservative in most cases; however, osteosynthesis with wires or flexible nails was used, followed by their removal in 4 patients; bone plates were applied in 4 patients; osteosynthesis with the Ilizarov apparatus was used in 3 cases, and intramedullary osteosynthesis with rigid rods in 4 patients. Regular administration of bisphosphonate preparations was conducted in 9 patients. Last dose of the drug was introduced at least 4 months before admission to surgical treatment. Thirteen patients had undergone reconstructive surgeries on the bones of lower extremities to correct the deformities. Conclusion Deformities of the lower limbs in patients with severe OI types are complex and multilevel, which implies the need for multilevel orthopedic interventions to correct them. High incidence of fractures and a complex nature of severe bone deformities result in the loss of the skills to move independently, or even its initial absence. Inadequate surgical interventions, untimely performed, non-telescopic nature of osteosynthesis, and absent bisphosphonate therapy contribute to the formation of additional orthopedic problems and aggravation of the loss of autonomy in such patients, which may complicate subsequent specific orthopedic surgical treatment

Situational problems as a means for forming the professional competencies of university students

© 2015 by the author(s). The purpose of this article aims to study the problem of forming students' competencies during the professional training in the university. The paper demonstrates a methodology for the development and application of situational problems as an effective means of forming the professional competencies of university students. The basis of the presented work is the ideas of the competence-based and the activity-based approaches, expert evaluation of the university students' educational activity. The article describes the technology for developing the situational problems, based on the analysis of the professional roles. This article is intended for educators, researchers, and heads of educational institutions that are into the formation and diagnostics of university students' professional competencies

Situational problems as a means for forming the professional competencies of university students

© 2015 by the author(s). The purpose of this article aims to study the problem of forming students' competencies during the professional training in the university. The paper demonstrates a methodology for the development and application of situational problems as an effective means of forming the professional competencies of university students. The basis of the presented work is the ideas of the competence-based and the activity-based approaches, expert evaluation of the university students' educational activity. The article describes the technology for developing the situational problems, based on the analysis of the professional roles. This article is intended for educators, researchers, and heads of educational institutions that are into the formation and diagnostics of university students' professional competencies

CONTENTS OF SOME OSTEOTROPIC GROWTH FACTORS AND OSTEOGENESIS MARKERS IN BLOOD OF PATIENTS WITH OSTEOGENESIS IMPERFECTa AND HYPOPHOSPHATEMIC RICKETS

In recent years there is intensive growth of the number of genetic diseases such as imperfect osteogenesis and hypophosphatemic rickets. Treatment of this category of patients includes surgical intervention, however in many cases there are recurrences of the disease and the patients have to undergo multiple surgeries. It is known that study of concentration of growth factors and regulatory polyproteins can help in understanding of pathogenetic course of genetic diseases. Material and methods. We studied growth factors and their receptors, bone markers and vitamin D by enzyme-linked immunoassay in blood serum of patients with hypophosphatemic rickets and osteogenesis imperfecta during surgical treatment. The analysis was carried out according to technique from manual to sets eBioscience (TGF-β1, TGF-β2, VEGF-A, VEGF-R2, VEGF-R3), Immunodiagnostic systems (IGF-1, osteocalcin, vitamin D, β-CrossLaps), Invitrogen (VEGF), RayBiotech Inc. (FGF-1, FGF-2, TGF-α, SCF, SCFsR), Mediagnost (IGF-2), QULDEL (pyridinoline), Enzo (DKK-1). Results. It has been established that concentration of growth factors IGF-1, VEGF, FGF-2, TGF-β1, TGF-β2 and their receptors VEGF-R3, SCFsR, bone markers osteocalcin, pyridinoline, as well as vitamin D in blood of patients of different groups at different stages of treatment varied. However, concentration of IGF-1, VEGF-R2 before surgery and ТGF-α on the 60th day, SCF on the 60th day in patients with hypophosphatemic rickets and osteogenesis imperfecta did not differ

A New Method for the Visualization of Living Dopaminergic Neurons and Prospects for Using It to Develop Targeted Drug Delivery to These Cells

This is the first study aiming to develop a method for the long-term visualization of living nigrostriatal dopaminergic neurons using 1-(2-(bis(4-fluorophenyl)methoxy)ethyl)-4-(3-phenylpropyl)piperazine-BODIPY (GBR-BP), the original fluorescent substance, which is a derivative of GBR-12909, a dopamine uptake inhibitor. This method is based on the authors’ hypothesis about the possibility of specifically internalizing into dopaminergic neurons substances with a high affinity for the dopamine transporter (DAT). Using a culture of mouse embryonic mesencephalic and LUHMES cells (human embryonic mesencephalic cells), as well as slices of the substantia nigra of adult mice, we have obtained evidence that GBR-BP is internalized specifically into dopaminergic neurons in association with DAT via a clathrin-dependent mechanism. Moreover, GBR-BP has been proven to be nontoxic. As we have shown in a primary culture of mouse metencephalon, GBR-BP is also specifically internalized into some noradrenergic and serotonergic neurons, but is not delivered to nonmonoaminergic neurons. Our data hold great promise for visualization of dopaminergic neurons in a mixed cell population to study their functioning, and can also be considered a new approach for the development of targeted drug delivery to dopaminergic neurons in pathology, including Parkinson’s disease