2 research outputs found
Mastocytosis in children: Clinicopathological study based on 35 cases
Immunohistochemical staining is useful in
the diagnosis of bone marrow infiltration in systemic
mastocytosis. However, it is not clear if antibody
staining may be helpful in the diagnosis of cutaneous
mastocytosis (CM). We studied the histological
appearance of CM in 35 pediatric patients. Cases were
assigned to three basic clinical groups: I - Urticaria
pigmentosa (UP, n=29); II - Mastocytomas (n=4); and III
- Diffuse Cutaneous Mastocytosis (DCM, n=2). The
analysis of clinical information revealed an association
between the presence of diarrhea and a higher number of
cells/field. Nine doubtful cases, all of them macules,
were selected based on the scarcity of mast cells (MC)
and the absence or rarity of other inflammatory cells. We
compared the number of cells identified in Giemsa and
immunohistochemical stains in definite and doubtful
cases. The intraclass correlation statistic tested the
concordance between each staining method. All 9
dubious cases according to the Giemsa stain had their
CM diagnosis confirmed by the immunohistochemistry
analysis. The intraclass correlation between Giemsa and
c-kit was good (0.7) when the number of MC was high.
However, there was no correlation between the mast
cells counts in the two different stains in the dubious
cases. The immunohistochemistry with c-kit might make
CM diagnosis easier, especially in the macular cases,
when there is a lower number of MC