Molecular datations from mtDNA sequences.

a<p>Nodes of the MJ network used to estimate TMRCAs from ρ statistic (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034423#pone-0034423-g003" target="_blank">Figure 3</a>).</p

Pairwise relationships between mtDNA, microsatellites and geographical distances in the Rhone drainage.

<p>Pairwise relationship between Fst/(1−Fst) (genetic distance) vs. geographical distances (km) using microsatellites (A) and mtDNA (B) or between microsatellites vs. mtDNA genetic distances in the Rhone drainage. The correlation coefficient (R) and the P-value (P) obtained from Mantel test are reported for each bivariate analysis.</p

Bayesian clustering analyses of microsatellites using Structure.

<p>Estimated population structure for population samples representative of <i>Telestes</i> genus (A) and <i>T. s. souffia</i> (B). “*” indicates K values retained from Δ<i>K </i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034423#pone.0034423-Evanno1" target="_blank">[67]</a> and Δ<i>Fst </i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034423#pone.0034423-Campana1" target="_blank">[64]</a> tests and when maximizing the posterior probability value <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034423#pone.0034423-Pritchard1" target="_blank">[60]</a>. Between parentheses, the number of convergent runs associated to the structure displayed.</p

Influence of group, environment, season and size on feeding behaviour.

<p>Distribution of the three classes of feeding behaviour (invertebrate eaters in purple, omnivores in orange and diatom eaters in deep green). Stacked bar chart showing the proportions of the various feeding behaviour classes by species and zone type in <b>A</b>), by season for each syntopic zone in <b>B</b>). Probability of GutVac (<i>y</i>-axis) as predicted by the multinomial model as a function of size in <b>C)</b> in which the <i>X</i>-axis corresponds to body size in cm, and we can read for example the longer specimens are more omnivor than herbivor.</p

Sampled <i>Telestes</i> taxa, population locations and sample sizes.

a<p>see main text for abbreviations.</p

Sampling map.

<p>Four allopatric stations (red for <i>P</i>. <i>toxostoma</i> and dark green for <i>C</i>. <i>nasus</i>) and 12 sympatric stations (the Rhône basin) were sampled. Furthermore, sympatric populations classified as allotopic stations (five; orange for <i>P</i>. <i>toxostoma</i> and light green for <i>C</i>. <i>nasus</i>) and syntopic station (blue stations; three for the Ardèche basin and four for the Durance basin). Allopatric and alltopic populations constituted the reference populations. This picture is a modified version of a copyright free picture of Daniel Dalet, available on <a href="http://www.histgeo.ac-aix-marseille.fr/" target="_blank">www.histgeo.ac-aix-marseille.fr</a>.</p

Location of the sampled <i>Telestes</i> populations with emphasize on the Rhone and French Mediterranean basins.

<p>For samples ID, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034423#pone-0034423-t001" target="_blank">Table 1</a>.</p

Summary of body deformations taking into account species, size, environment and their interaction.

<p>A) Ontogenic deformations f<i>or C</i>. <i>nasus</i> in reference condition, B) Ontogenic deformations f<i>or P</i>. <i>toxostoma</i> in reference condition, C) Interspecies deformations in reference condition, D) Deformation from reference condition to Durance for both species, E) Deformation from reference condition to Ardèche for both species. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0142592#pone.0142592.s005" target="_blank">S5 Fig</a>. for more details. The width of an arrow is positively related to the intensity of the deformation.</p

Phylogenetic and multivariate analyses of microsatellites data.

<p>Unrooted Maximum Likelihood tree based on microsatellites Dc distances (A) and FCA scatter plots for <i>T. s. souffia</i> population samples (B) and population samples representative of <i>Telestes</i> genus (C).</p

Median-Joining network of the <i>T. souffia</i> mitochondrial haplotypes.

<p>Population samples ID detailed in haplotypes charts when necessary.</p