Die Konjunktiv-II-Bildung im Kontext von Partikelverben in den Basisdialekten Salzburgs

Focusing on phrasal verbs such as einbringen ‘to harvest’ or abbringen ‘to dissuade’, the aim of the present exploratory apparent-time study is to uncover factors affecting the subjunctive II formation in the traditional base dialects of Salzburg (Austria). Depending on whether the subjunctive II formation is synthetic or periphrastic, phrasal verbs are formed in contact position (e. g., würde/täte abbringen ‘would dissuade’) or in distance position (e. g. brächte ab ‘would dissuade’) between particle and verb stem. In order to examine which subjunctive II variants are used for 15 phrasal verbs in the traditional dialects of Salzburg, an indirect survey was carried out with 25 informants in six rural locations. These villages are spread across the three dialect areas (West-Central Bavarian, South-Central Bavarian, and South Bavarian) cutting through the federal state of Salzburg. Furthermore, the subjunctive II formation of the phrasal verbs is compared to that of the simple verbs (e. g., bringen ‘to bring’) corresponding to the derivational bases of the respective phrasal verbs investigated (e. g., einbringen or abbringen). In addition to linguistic and areal factors, sociolinguistic factors (age and gender) are taken into account. Results show that the subjunctive II formation of phrasal verbs differs significantly from that of simple verbs: compared to the corresponding simple verbs, the informants used the phrasal verbs significantly more often with periphrastic variants. It is argued that this tendency is related to specific semantic-lexical and morpho-syntactic properties of phrasal verbs. Regarding the areal distribution, there are differences between the individual locations. These differences do not correlate with the traditional dialect regions but can be traced back to the socio-demographic characteristics of the locations (number of inhabitants, transport connections, tourism etc.). Moreover, age proves to be a relevant factor: there is a statistically significant difference in both, simple verbs and phrasal verbs, with the younger informants preferring the periphrastic variants

Sulfolobus – A Potential Key Organism in Future Biotechnology

Extremophilic organisms represent a potentially valuable resource for the development of novel bioprocesses. They can act as a source for stable enzymes and unique biomaterials. Extremophiles are capable of carrying out microbial processes and biotransformations under extremely hostile conditions. Extreme thermoacidophilic members of the well-characterized genus Sulfolobus are outstanding in their ability to thrive at both high temperatures and low pH. This review gives an overview of the biological system Sulfolobus including its central carbon metabolism and the development of tools for its genetic manipulation. We highlight findings of commercial relevance and focus on potential industrial applications. Finally, the current state of bioreactor cultivations is summarized and we discuss the use of Sulfolobus species in biorefinery applications

Increased Expression of Micro-RNA-23a Mediates Chemoresistance to Cytarabine in Acute Myeloid Leukemia

Resistance to chemotherapy is one of the primary obstacles in acute myeloid leukemia (AML) therapy. Micro-RNA-23a (miR-23a) is frequently deregulated in AML and has been linked to chemoresistance in solid cancers. We, therefore, studied its role in chemoresistance to cytarabine (AraC), which forms the backbone of all cytostatic AML treatments. Initially, we assessed AraC sensitivity in three AML cell lines following miR-23a overexpression/knockdown using MTT-cell viability and soft-agar colony-formation assays. Overexpression of miR-23a decreased the sensitivity to AraC, whereas its knockdown had the opposite effect. Analysis of clinical data revealed that high miR-23a expression correlated with relapsed/refractory (R/R) AML disease stages, the leukemic stem cell compartment, as well as with inferior overall survival (OS) and event-free survival (EFS) in AraC-treated patients. Mechanistically, we demonstrate that miR-23a targets and downregulates topoisomerase-2-beta (TOP2B), and that TOP2B knockdown mediates AraC chemoresistance as well. Likewise, low TOP2B expression also correlated with R/R-AML disease stages and inferior EFS/OS. In conclusion, we show that increased expression of miR-23a mediates chemoresistance to AraC in AML and that it correlates with an inferior outcome in AraC-treated AML patients. We further demonstrate that miR-23a causes the downregulation of TOP2B, which is likely to mediate its effects on AraC sensitivity