A Comprehensive Analysis of Gene Expression and Genomic Alterations in a Newly Formed Autotetraploid of \u3cem\u3ePaspalum Notatum\u3c/em\u3e

The proportion of angiosperms that have experienced one or more episodes of chromosome doubling is estimated to be of the order of 50-70%. This prominence of polyploidy probably implies some adaptive significance. The emergence of novel phenotypes could allow polyploids to enter new niches or enhance their chances of being selected for use in agriculture. Although the causes of novel variation in polyploids are not well understood, they could involve changes in gene expression through dosage-regulation, altered regulatory interactions and rapid genetic/epigenetic changes. The objective of this work was to carry out an extensive transcript profiling and genome analysis of a diploid genotype of the pasture grass Paspalum notatum and its tetraploid derivative obtained after colchicine treatment

Genetic transfer from several apomictic tetraploid Paspalum species to an elite group of sexual plants

Basic findings from classical genetic studies are available for exploiting apomixis in the breeding of several forage grass genera. Most Paspalum species are multiploid with a sexual diploid cytotype and conspecific apomictic polyploid (mainly tetraploid) cytotypes. Experimental tetraploidized diploids reproduce sexually and, when crossed with natural apomictic tetraploids, yield hybrid populations that segregate for reproductive mode. Genetic studies indicated that apomixis is inherited as a monogenic dominant factor. We recombined 50 selected sexual hybrids obtained from crosses between a tetraploidized sexual genotype of P. plicatulum and 9 natural apomictic tetraploid accessions of 6 species of the Plicatula group. A synthetic sexual tetraploid population (SSTP) of 600 individuals from mixed seed of the 50 intercrossed hybrids was space-planted in the field. Based on evaluations of plant vigor, seed set, ergot tolerance, regrowth after flowering, and cold tolerance, 31 plants were selected. Crosses between most selected plants and two testers, which belonged to P. guenoarum, were performed, and the generated progeny was planted into the field following a randomized block design with 3 replications. The progeny test was evaluated for seed fertility, biomass yield, and cold tolerance. This procedure allowed selection of 10 elite plants from the SSTP. These plants should contain genes recombined from six apomictic species, without the genetic determinants for apomixis. They may be polycrossed to generate an improved sexual population, or crossed with other apomictic genotypes to obtain improved apomictic hybrids.Fil: Novo, Patricia Elda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Acuña, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Urbani, Mario Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Galdeano, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Quarin, Camilo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentin

A Core AFLP Map of Aposporic Tetraploid \u3cem\u3ePaspalum Notatum\u3c/em\u3e (Bahiagrass)

Paspalum notatum (Bahiagrass) is a perennial rhizomatous species that reproduces by aposporous apomixis. Tetraploid races (2n=4x=40) are widely distributed from Central to South America and constitute one of the most valuable natural forage grasses for the subtropical areas of Paraguay, southern Brazil and north-eastern Argentina. Apospory in the species is controlled by a single locus, which exhibits a distorted segregation ratio. The objectives of this work were to develop a core genetic linkage map of the species by using AFLP markers and characterize the genomic region related to apospory