Effect of codon optimization of <i>IgASE1</i> on fatty acid composition in transgenic Arabidopsis.

<p>Effect of codon optimization of <i>IgASE1</i> on fatty acid composition in transgenic Arabidopsis.</p

Effect of codon optimization of PinD6 on fatty acid conversion rate in transgenic yeast cells.

<p>(a) Codon changes in the first 16 amino acids at the N-terminus; (b) Codon changes of CGC_Arg to AGA_Arg at positions 9, 76, 376 and 433; (c) Fatty acid conversion rates of WT and codon-optimized variants of PinD6 in transgenic yeast. WT, 4R^op, N^op and N^op+4R^op represents transgenic yeast harboring the wild-type, four CGC to AGA-optimized, N-terminal 16 codons optimized and the N-terminal 16 codons plus the four CGC to AGA optimized PinD6, respectively. Each value represents the mean ± standard deviation from three independent repeats. Different letters indicate statistically different values after one-way ANOVA.</p

Primers used in this study.

<p>Primers used in this study.</p

Effect of codon optimization of IgASE1 on fatty acid conversion in transgenic <i>Arabidopsis</i>.

<p>(a) ω3 fatty acid conversion rates in transgenic <i>Arabidopsis</i> expressing WT and the codon optimized IgASE1; (b) ω6 fatty acid conversion rates in transgenic <i>Arabidopsis</i>. Number of transgenic plants per 10 transgenics having different fatty acid conversion rates are shown. Conversion rate of ω3 (ALA to ETrA) or ω6 (LA to EDA) fatty acids are calculated as: (product/product+substrate)x100.</p

Positions of nucleotides that were changed for codon optimization of <i>IgASE1</i>.

<p>(a) Codon changes in the first 15 amino acids at the N-terminus; (b) Codon changes of CGC_Arg at positions 10, 35 and 84.</p

Fatty acid analysis of WT and transgenic yeast cells expressing codon-optimized variants of PinD6.

<p>Fatty acid analysis of WT and transgenic yeast cells expressing codon-optimized variants of PinD6.</p