1 research outputs found
Guidance of Stem Cells to a Target Destination in Vivo by Magnetic Nanoparticles in a Magnetic Field
Stem
cells contribute to physiological processes such as postischemic
neovascularization and vascular re-endothelialization, which help
regenerate myocardial defects or repair vascular injury. However,
therapeutic efficacy of stem cell transplantation is often limited
by inefficient homing of systemically administered cells, which results
in a low number of cells accumulating at sites of pathology. In this
study, anti-CD34 antibody-coated magnetic nanoparticles (Fe<sub>3</sub>O<sub>4</sub>@PEG-CD34) are shown to have high affinity to stem cells.
The results of hemolysis rate and activated partial thromboplastin
time (APTT) tests indicate that such nanoparticle may be used safely
in the blood system. In vitro studies showed that a nanoparticle concentration
of 100 μg/mL gives rise to a significant increase in cell retention
using an applicable permanent magnet, exerting minimal negative effect
on cell viability and migration. Subsequent in vivo studies indicate
that nanopartical can specifically bind stem cells with good magnetic
response. Anti-CD34 antibody coated magnetic nanoparticle may be used
to help deliver stem cells to a lesion site in the body for better
treatment