Basic Research on Rockburst Control Technology for Deep Well Filling of Municipal Solid Waste

AbstractGiven the scarcity of raw materials for rockburst prevention in filling mining and the lack of space for disposal of large amount of municipal waste, the feasibility of preparing filling materials for rockburst mines from stale waste was investigated by laboratory tests and theoretical analysis. On this basis, the process of preparing filling materials from stale garbage was proposed, and corresponding equipment were developed to prepare stale garbage filling mass. According to the characteristics and uses of the stale waste filling materials, two processes of volume filling and strength filling are proposed, and the key technology of stale garbage filling to control rockburst was designed. The following conclusions were drawn: stale garbage can be made into mine filling material because of its composition, strength, and shape. The process of preparing mine filling materials from obsolete waste includes crushing, screening, compression, and packaging. The equipment suitable for the process includes crushing-screening, compression-forming, and sealing-packaging integrated equipment. The equipment has realized effective screening, compression, and bulk packaging of stale garbage, so that the stale garbage filling mass can meet the requirements of environmental protection and strength. Strength filling is a filling method that uses the strength of stale garbage filling mass to protect the overlying strata from or less damage, thereby reducing the stress concentration in the coal face and reducing the risk of rockburst occurring. Volume filling mainly depends on the volume of the filling mass, with the main purpose of reducing the stress concentration in the roadway surrounding rock. The rockburst mine filling technology of stale garbage is support track filling technology and bag filling technology, and the deep well sealing of stale garbage is block stacking technology. The deep well filling mining key technologies provide a new approach to against rockburst and treat large amounts of municipal waste

Research on Mechanism of Rock Burst in Key Working Faces under Thick Magmatite in Deep Mine

The rock burst of key working faces under the thick hard rock in deep mine significantly threatens the mining safety of deep mine. In this study, the key working faces under typical deeply buried thick magmatite were adopted as the engineering background. The mine pressure characteristics during the mining in key working faces under thick magmatite in deep mine were measured and analyzed. Then, the evolution of overburden strata structure under the control of thick magmatite was explored based on the theory of mine pressure to conclude that the horizontal “carrier” load of broken rock beam, the vertical “loader” load, and the shock bump load from thick magmatite fracture are main sources of force behind the burst. Finally, the mechanism of rock burst was studied on the basis of the balanced relationship between loading and bearing. According to the results of research, the burst in key working faces under thick magmatite in deep mine was actually the instability burst of the key working face block. The bearing capacity and load of key working face block were constantly changing during the unstable movement of thick magmatite. The rock burst would occur in the event of any instability during the dynamic confrontation of “loading-bearing”. As per different burst sources, it could be divided into flexural loading burst of thick magmatite and shock bump burst of thick magmatite fracture. The mechanical conditions for each of the two bursts and the width calculation formula for the key working face free from overall instability burst were deduced. The research results were applied to key working face 12310. Meanwhile, the purpose of safe production following the principle of “No disaster in bumps, no harm under burst” was realized by implementing the “Four Keys” comprehensive prevention and control measures of “key monitoring + key speed reduction + key pressure relief + key support”

Facilitation of Expression and Purification of an Antimicrobial Peptide by Fusion with Baculoviral Polyhedrin in Escherichia coli

Several fusion strategies have been developed for the expression and purification of small antimicrobial peptides (AMPs) in recombinant bacterial expression systems. However, some of these efforts have been limited by product toxicity to host cells, product proteolysis, low expression levels, poor recovery yields, and sometimes an absence of posttranslational modifications required for biological activity. For the present work, we investigated the use of the baculoviral polyhedrin (Polh) protein as a novel fusion partner for the production of a model AMP (halocidin 18-amino-acid subunit; Hal18) in Escherichia coli. The useful solubility properties of Polh as a fusion partner facilitated the expression of the Polh-Hal18 fusion protein (∼33.6 kDa) by forming insoluble inclusion bodies in E. coli which could easily be purified by inclusion body isolation and affinity purification using the fused hexahistidine tag. The recombinant Hal18 AMP (∼2 kDa) could then be cleaved with hydroxylamine from the fusion protein and easily recovered by simple dialysis and centrifugation. This was facilitated by the fact that Polh was soluble during the alkaline cleavage reaction but became insoluble during dialysis at a neutral pH. Reverse-phase high-performance liquid chromatography was used to further purify the separated recombinant Hal18, giving a final yield of 30% with >90% purity. Importantly, recombinant and synthetic Hal18 peptides showed nearly identical antimicrobial activities against E. coli and Staphylococcus aureus, which were used as representative gram-negative and gram-positive bacteria, respectively. These results demonstrate that baculoviral Polh can provide an efficient and facile platform for the production or functional study of target AMPs

Increased secondary aerosol contribution and possible processing on polluted winter days in China

China experiences severe particulate pollution, especially in winter, and determining the characteristics of particulate matter (PM) during pollution events is imperative for understanding the sources and causes of the pollution. However, inconsistencies have been found in the aerosol composition, sources and secondary processing among reported studies. Modern meta-analysis was used to probe the PM chemical characteristics and processing in winter at four representative regions of China, and the first finding was that secondary aerosol formation was the major effect factor for PM pollution. The secondary inorganic species behaved differently in the four regions: sulfate, nitrate, and ammonium increased in the Beijing-Tianjin-Hebei (BTH) and Guanzhong (GZ) areas, but only nitrate increased in the Pearl River Delta (PRD) and Yangtze River Delta (YRD) regions. The increased production of secondary organic aerosol (SOA) was probably caused by aqueous-phase processing in the GZ and BTH regions and by photochemical reactions in the PRD. Finally, we suggest future AMS/ACSM observations should focus on the aerosol characteristics in rural areas in winter in China.</p