A dynamic model of UVR8 photoreceptor signaling in UV-B acclimated Arabidopsis

The photoreceptor UVR8 mediates numerous photomorphogenic responses of plants to UV‐B wavelengths by regulating transcription. Studies with purified UVR8 and seedlings not previously exposed to UV‐B have generated a model for UVR8 action in which dimeric UVR8 rapidly monomerises in response to UV‐B exposure to initiate signaling. However, the mechanism of UVR8 action in UV‐B‐acclimated plants growing under photoperiodic conditions, where UVR8 exists in a dimer/monomer photo‐equilibrium, is poorly understood. We examined UVR8 dimer/monomer status, gene expression responses, amounts of key UVR8 signaling proteins and their interactions with UVR8 in UV‐B‐acclimated Arabidopsis. We show that in UV‐B‐acclimated plants UVR8 can mediate a response to a 15‐fold increase in UV‐B without any increase in abundance of UVR8 monomer. Following transfer to elevated UV‐B, monomers show increased interaction with both COP1, to initiate signaling, and RUP2, to maintain the photo‐equilibrium when the dimer/monomer cycling rate increases. Native RUP1 is present in low abundance compared with RUP2. We present a model for UVR8 action in UV‐B‐acclimated plants growing in photoperiodic conditions that incorporates dimer and monomer photoreception, dimer/monomer cycling, abundance of native COP1 and RUP proteins, and interactions of the monomer population with COP1, RUP2 and potentially other proteins

VREMENSKI UTJECAJ NA MEĐUDJELOVANJE ODLAGALIŠTA I TLA TE NJIHOV ELASTIČNO-VISKOZNO-PLASTIČNI MODEL

Pile-soil interaction is considered to be one of the most important problems in the study of the mechanical behaviour of pile foundation. In this paper, the lateral friction resistance and pile-soil relative displacement of bridge piles in deep soft soils are monitored for an extended period by using concrete strain gauges that are embedded in the test pile. Field test results show that both the pile lateral frictional resistance and pile-soil relative displacement increase along with time, while the pile-soil interaction demonstrates a time effect. Therefore, an elastic-viscoplastic constitutive model must be established to better simulate the time-dependent mechanical behaviour of the pile-soil contact. Based on the Goodman model, we developed an elastic-viscoplastic interface constitutive model of the pile-soil interface into the FRIC subroutine through ABAQUS software, which is one of the most commonly used finite element analysis softwares in the world, to simulate a well-recorded pile test in deep soft soils. The calculated pile lateral frictional resistance and pile-soil relative displacement are close to the measured values, and the ability of the interface model to describe the changes in the shear stress-strain of the pile-soil interface along with time is validated.Međudjelovanje odlagališta (jalovine) i tla jedan je od najvažnijih problema kod izučavanja mehaničkih svojstava takvih odlagališta. Praćeno je bočno smicanje te otpor na dodiru mekoga tla i odloženoga materijala unutar njega tijekom vremena. Unutar testnoga odlagališta također su postavljeni betonski blokovi za mjerenje naprezanja. Rezultati su pokazali kako su bočna smicanja između odloženoga materijala i tla ovisna o vremenu. Ta zavisnost modelirana je elastično-viskozno-plastičnim modelom, čime je opisana ovisnost mehaničkih pojava o vremenu. Prikazani model deriviran je iz Goodmanova, te je rutinom (FRIC) opisan u programskome paketu (ABAQUS), koji je ujedno i najčešći program za analizu konačnih elemenata s ovakvom namjenom. Izračunani otpor na bočno smicanje te relativni pomaci u odlagalištu vrlo su slični onima izmjerenim na mjestu eksperimentalnoga testiranja. Također je potvrđena mogućnost predviđanja modelom promjena nastalih zbog naprezanja tijekom vremena

Quantum Nonlinear Effect in Dissipatively Coupled Optomechanical System

A full-quantum approach is used to study quantum nonlinear properties of a compound Michelson-Sagnac interferometer optomechanical system. The effective Hamiltonian shows that both dissipative and dispersive couplings possess imaginary- and real-Kerr nonlinearities. And unexpectedly, the nonlinearities caused by the dissipative coupling have non-Hermitian Hamiltonian-like properties. It can protect the quantum nature of the dispersive coupling beyond the traditional dissipation of the system. This protection mechanism allows the system to exhibit strong quantum nonlinear effects in the parameter region of the hyperbolic function $J^2 = \Delta_c \Delta_e$. Moreover, we can obtain strong anti-bunching effects whether in strong or weak coupling regimes with the help of the dispersive and dissipative couplings jointly. It may provide a new perspective to experimentally realize and study the strong quantum nonlinear effects

Quantized Feedback Control for Networked Control Systems Under Communication Constraints

This paper investigates the feedback stabilization problem for networked control systems (NCSs) with unbound process noise, where sensors and controllers are connected via noiseless digital channels carrying a finite number of bits per unit time. A sufficient condition for stabilization of NCSs, which relies on a variable-rate digital link used to transmit state measurements, is derived. A lower bound of data rates, above which there exists a quantization, coding and control scheme to guarantee both stabilization and a prescribed control performance of the unstable discrete-time plant, is presented. An illustrative example is given to demonstrate the effectiveness of the proposed method

Identification of genotype 4 Hepatitis E virus binding proteins on swine liver cells

Hepatitis E virus (HEV) is a zoonotic pathogen of which several species of animal were reported as reservoirs. Swine stands out as the major reservoir for HEV infection in humans, as suggested by the close genetic relationship of swine and human virus and cross-species infection of HEV. Up to now, the mechanism of cross-species infection of HEV from swine to humans is still unclear. This study sought to identify receptor element for genotype 4 HEV on swine liver cells using the viral overlay protein binding assay (VOPBA) technique and Mass Spectrometry fingerprinting. A single virus binding band with natural molecular weight about 55 kDa was observed, and mass spectrometry revealed that this virus binding band contained 31 different proteins. Infection inhibition assay suggested that this 55 kDa protein could prevent HEV from infecting its susceptible A549 cell line, which was further confirmed by the HEV genome detecting in the inoculated cells. Further research should be performed to elucidate the accurate receptor of HEV on the swine liver cells

Synchronous Gastric Carcinoma and Nodal Malignant Lymphoma: A Rare Case Report and Literature Review

Synchronous double malignancies of gastric carcinoma (GC) and malignant lymphoma (ML) are rare and very difficult to treat. We report a case of synchronous GC and nodal ML, regarding which clinical and pathological features and treatment are discussed. A 68-year-old woman with a history of inguinal hernia was admitted for abdominal pain and high fever and subsequently underwent herniorrhaphy, but the fever remained. Computerized tomography showed a stomach mass and multiple enlarged lymph nodes in the abdominal cavity and inguinal regions. Gastric adenocarcinoma coexistent with advanced in situ follicular lymphoma was confirmed by endoscopy, biopsy of inguinal lymph nodes and bone marrow examination. Two chemotherapy regimens, R-CHOP (rituximab, cyclophosphamide, perarubicin, vincristine and prednisone) and systemic therapy (5-fluorouracil and calcium folinate) combined with regional perfusion (oxaliplatin and etoposide) through the left gastric artery were performed at intervals against ML and GC, respectively. Partial remission in both tumors was achieved after 4 courses of treatment, but the patient finally died of heart failure. Scrupulous biopsy of non-draining lymph nodes in patients with gastrointestinal carcinomas is supposed to improve the diagnostic rate of simultaneous nodal ML. The interval chemotherapy strategy with two independent regimens is beneficial for such patients, especially for those unable to tolerate major surgery

Rapid Remission in Peripheral T-Cell Lymphoma of the Nasal Type by the Bortezomib plus CHOP Therapy

Peripheral T-cell lymphoma (PTCL) is rare and difficult to treat for its high relapse rate. The authors report a case of PTCL of the skin, regarding which clinical and pathological features, treatment, and prognosis were discussed. A 66-year-old woman was admitted with complaints of enlarging erythematous noduloplaques on the right anterior tibial skin for one year and similar lesions on the left for 6 months. Surgical resection of right leg lesion and biopsy of enlarged inguinal lymph nodes histologically indicated a PTCL of the nasal type. The patient was treated by CHOP plus bortezomib, reached complete remission just after two courses of chemotherapy and then received another two as consolidation. The patient remained in remission for 11 months until local relapse. As for cutaneous lesions, detailed lymph node examination and prompt tissue biopsy are judicious choices prior to any medical management. The chemotherapy consisting of bortezomib and CHOP is safe and efficient in PTCL of the skin