20 research outputs found
Carbon Dioxide Microbubble Bursting Ionization Mass Spectrometry
Aerosols generated by bubble bursting have been proved
to promote
the extraction of analytes and have ultrahigh electric fields at their
water–air interfaces. This study presented a simple and efficient
ionization method, carbon dioxide microbubble bursting ionization
(CDMBI), without the presence of an exogenous electric field (namely,
zero voltage), by simulating the interfacial chemistries of sea spray
aerosols. In CDMBI, microbubbles are generated in situ by continuous input of carbon dioxide into an aqueous solution containing
low-concentration analytes. The microbubbles extract low- and high-polarity
analytes as they pass through the aqueous solution. Upon reaching
the water–air interface, these microbubbles burst to produce
charged aerosol microdroplets with an average diameter of 260 μm
(8.1–10.4 nL in volume), which are immediately transferred
to a mass spectrometer for the detection and identification of extracted
analytes. The above analytical process occurs every 4.2 s with a stable
total ion chromatogram (relative standard deviation: 9.4%) recorded.
CDMBI mass spectrometry (CDMBI-MS) can detect surface-active organic
compounds in aerosol microdroplets, such as perfluorooctanoic acid,
free fatty acids epoxidized by bubble bursting, sterols, and lecithins
in soybean and egg, with the limit of detection reaching the level
of fg/mL. In addition, coupling CDMBI-MS with an exogenous voltage
yields relatively weak gains in ionization efficiency and sensitivity
of analysis. The results suggested that CDMBI can simultaneously accomplish
both bubbling extraction and microbubble bursting ionization. The
mechanism of CDMBI involves bubbling extraction, proton transfer,
inlet ionization, and electrospray-like ionization. Overall, CDMBI-MS
can work in both positive and negative ion modes without necessarily
needing an exogenous high electric field for ionization and quickly
detect trace surface-active analytes in aqueous solutions
Additional file 2: of Human-induced pluripotent stem cell-derived macrophages and their immunological function in response to tuberculosis infection
Figure S2. Immunofluorescence images showing the positive expression of Mφ lineage markers CD14 in iPS-Mφ (A), THP-1-Mφ (B) and ES-Mφ (C). Nuclei are labeled with DAPI. Bar = 100 μm. (TIFF 1337 kb
Additional file 5: of Human-induced pluripotent stem cell-derived macrophages and their immunological function in response to tuberculosis infection
Figure S5. Immunofluorescence images showing the positive expression of Mφ lineage markers MHC-II in iPS-Mφ (A), THP-1-Mφ (B) and ES-Mφ (C). Nuclei are labeled with DAPI. Bar = 100 μm. (TIFF 1462 kb
Additional file 3: of Human-induced pluripotent stem cell-derived macrophages and their immunological function in response to tuberculosis infection
Figure S3. Immunofluorescence images showing the positive expression of Mφ lineage markers CD40 in iPS-Mφ (A), THP-1-Mφ (B) and ES-Mφ (C). Nuclei are labeled with DAPI. Bar = 100 μm. (TIFF 1548 kb
Image_2_NETosis is critical in patients with severe community-acquired pneumonia.pdf
Pneumonia is the fourth leading cause of death globally, and the reason for the high mortality rate of patients with severe community-acquired pneumonia (SCAP) remains elusive. Corticosteroid treatment reduces mortality in adults with SCAP but can cause numerous adverse events. Therefore, novel therapeutic targets need to be explored and new adjunctive immune drugs are urgently required. We analyzed the transcriptome data of peripheral blood leukocytes from patients with SCAP and healthy controls from three perspectives: differentially expressed genes, predicted functions of differentially expressed long non-coding RNAs, and transcriptional read-through. We discovered that the NETosis pathway was top-ranked in patients with SCAP caused by diverse kinds of pathogens. This provides a potential therapeutic strategy for treating patients. Furthermore, we calculated the correlation between the expression of genes involved in NETosis and the ratio of arterial oxygen partial pressure to fractional inspired oxygen. We identified four novel potential therapeutic targets for NETosis in patients with SCAP, including H4C15, H3-5, DNASE1, and PRKCB. In addition, a higher occurrence of transcriptional read-through is associated with a worse outcome in patients with SCAP, which probably can explain the high mortality rate of patients with SCAP.</p
Table_2_NETosis is critical in patients with severe community-acquired pneumonia.xls
Pneumonia is the fourth leading cause of death globally, and the reason for the high mortality rate of patients with severe community-acquired pneumonia (SCAP) remains elusive. Corticosteroid treatment reduces mortality in adults with SCAP but can cause numerous adverse events. Therefore, novel therapeutic targets need to be explored and new adjunctive immune drugs are urgently required. We analyzed the transcriptome data of peripheral blood leukocytes from patients with SCAP and healthy controls from three perspectives: differentially expressed genes, predicted functions of differentially expressed long non-coding RNAs, and transcriptional read-through. We discovered that the NETosis pathway was top-ranked in patients with SCAP caused by diverse kinds of pathogens. This provides a potential therapeutic strategy for treating patients. Furthermore, we calculated the correlation between the expression of genes involved in NETosis and the ratio of arterial oxygen partial pressure to fractional inspired oxygen. We identified four novel potential therapeutic targets for NETosis in patients with SCAP, including H4C15, H3-5, DNASE1, and PRKCB. In addition, a higher occurrence of transcriptional read-through is associated with a worse outcome in patients with SCAP, which probably can explain the high mortality rate of patients with SCAP.</p
Table_1_NETosis is critical in patients with severe community-acquired pneumonia.xls
Pneumonia is the fourth leading cause of death globally, and the reason for the high mortality rate of patients with severe community-acquired pneumonia (SCAP) remains elusive. Corticosteroid treatment reduces mortality in adults with SCAP but can cause numerous adverse events. Therefore, novel therapeutic targets need to be explored and new adjunctive immune drugs are urgently required. We analyzed the transcriptome data of peripheral blood leukocytes from patients with SCAP and healthy controls from three perspectives: differentially expressed genes, predicted functions of differentially expressed long non-coding RNAs, and transcriptional read-through. We discovered that the NETosis pathway was top-ranked in patients with SCAP caused by diverse kinds of pathogens. This provides a potential therapeutic strategy for treating patients. Furthermore, we calculated the correlation between the expression of genes involved in NETosis and the ratio of arterial oxygen partial pressure to fractional inspired oxygen. We identified four novel potential therapeutic targets for NETosis in patients with SCAP, including H4C15, H3-5, DNASE1, and PRKCB. In addition, a higher occurrence of transcriptional read-through is associated with a worse outcome in patients with SCAP, which probably can explain the high mortality rate of patients with SCAP.</p
Table_5_NETosis is critical in patients with severe community-acquired pneumonia.xls
Pneumonia is the fourth leading cause of death globally, and the reason for the high mortality rate of patients with severe community-acquired pneumonia (SCAP) remains elusive. Corticosteroid treatment reduces mortality in adults with SCAP but can cause numerous adverse events. Therefore, novel therapeutic targets need to be explored and new adjunctive immune drugs are urgently required. We analyzed the transcriptome data of peripheral blood leukocytes from patients with SCAP and healthy controls from three perspectives: differentially expressed genes, predicted functions of differentially expressed long non-coding RNAs, and transcriptional read-through. We discovered that the NETosis pathway was top-ranked in patients with SCAP caused by diverse kinds of pathogens. This provides a potential therapeutic strategy for treating patients. Furthermore, we calculated the correlation between the expression of genes involved in NETosis and the ratio of arterial oxygen partial pressure to fractional inspired oxygen. We identified four novel potential therapeutic targets for NETosis in patients with SCAP, including H4C15, H3-5, DNASE1, and PRKCB. In addition, a higher occurrence of transcriptional read-through is associated with a worse outcome in patients with SCAP, which probably can explain the high mortality rate of patients with SCAP.</p
Table_3_NETosis is critical in patients with severe community-acquired pneumonia.xls
Pneumonia is the fourth leading cause of death globally, and the reason for the high mortality rate of patients with severe community-acquired pneumonia (SCAP) remains elusive. Corticosteroid treatment reduces mortality in adults with SCAP but can cause numerous adverse events. Therefore, novel therapeutic targets need to be explored and new adjunctive immune drugs are urgently required. We analyzed the transcriptome data of peripheral blood leukocytes from patients with SCAP and healthy controls from three perspectives: differentially expressed genes, predicted functions of differentially expressed long non-coding RNAs, and transcriptional read-through. We discovered that the NETosis pathway was top-ranked in patients with SCAP caused by diverse kinds of pathogens. This provides a potential therapeutic strategy for treating patients. Furthermore, we calculated the correlation between the expression of genes involved in NETosis and the ratio of arterial oxygen partial pressure to fractional inspired oxygen. We identified four novel potential therapeutic targets for NETosis in patients with SCAP, including H4C15, H3-5, DNASE1, and PRKCB. In addition, a higher occurrence of transcriptional read-through is associated with a worse outcome in patients with SCAP, which probably can explain the high mortality rate of patients with SCAP.</p
Table_6_NETosis is critical in patients with severe community-acquired pneumonia.xls
Pneumonia is the fourth leading cause of death globally, and the reason for the high mortality rate of patients with severe community-acquired pneumonia (SCAP) remains elusive. Corticosteroid treatment reduces mortality in adults with SCAP but can cause numerous adverse events. Therefore, novel therapeutic targets need to be explored and new adjunctive immune drugs are urgently required. We analyzed the transcriptome data of peripheral blood leukocytes from patients with SCAP and healthy controls from three perspectives: differentially expressed genes, predicted functions of differentially expressed long non-coding RNAs, and transcriptional read-through. We discovered that the NETosis pathway was top-ranked in patients with SCAP caused by diverse kinds of pathogens. This provides a potential therapeutic strategy for treating patients. Furthermore, we calculated the correlation between the expression of genes involved in NETosis and the ratio of arterial oxygen partial pressure to fractional inspired oxygen. We identified four novel potential therapeutic targets for NETosis in patients with SCAP, including H4C15, H3-5, DNASE1, and PRKCB. In addition, a higher occurrence of transcriptional read-through is associated with a worse outcome in patients with SCAP, which probably can explain the high mortality rate of patients with SCAP.</p