3 research outputs found
The Use of CFSE-like Dyes for Measuring Lymphocyte Proliferation : Experimental Considerations and Biological Variables
The measurement of CFSE dilution by flow cytometry is a powerful experimental tool to
measure lymphocyte proliferation. CFSE fluorescence precisely halves after each cell
division in a highly predictable manner and is thus highly amenable to mathematical
modelling. However, there are several biological and experimental conditions that can
affect the quality of the proliferation data generated, which may be important to consider
when modelling dye dilution data sets. Here we overview several of these variables
including the type of fluorescent dye used to monitor cell division, dye labelling
methodology, lymphocyte subset differences, in vitro versus in vivo experimental assays,
cell autofluorescence, and dye transfer between cells