2,141 research outputs found
Practical teaching reform of electrical major in colleges and universities in the new era
With the steady progress of a new round of energy reform, the rapid development of smart grid, the demand for high quality
electrical talents will increase. The course “Power system analysis” occupies a relatively important position in electrical majors, and the
course takes into account both theory and practice. Therefore, teachers should take the initiative to innovate their own teaching ideas, pay
attention to practical teaching, and promote the connection between theoretical teaching and practical teaching, so as to comprehensively
improve the teaching quality of the course and cultivate the high-quality talents needed by the industry. In this regard, this paper probes into
the practical teaching reform of electrical major in colleges and universities in the new era, for reference only
Cloning and expression analysis of potassium channel gene NKT3 from Nicotiana tabacum
Potassium (K+) is the predominant inorganic ion of plant cells. K+ channels in higher plant cells play an important role in regulating the influx and efflux of K+ from cells, and activity of these channels might be involved in plant stress resistance. A completely new K+ channel gene of Nicotiana tabacum was obtained through homologous cloning strategy. The complete cDNA sequence was submitted to the National Center for Biotechnology Information (NCBI) GenBank, designated as NKT3 and the accession number is FJ230956. The phylogenetic analysis indicated that NKT3 is located at the branch of weak-inwardly rectifying K+ channels and might be a member of the Shaker family. The spatial and temporal expression of the gene was also investigated. NKT3 is expressed abundantly in the roots, while little in the leaves of N. tabacum. It might be involved in the process of K+ acquirement and release in tobacco roots.Keywords: Potassium channel gene, NKT3, RACE, Nicotiana tabacu
New Reassortant H5N6 Highly Pathogenic Avian Influenza Viruses in Southern China, 2014
New reassortant H5N6 highly pathogenic avian influenza viruses were isolated from apparently healthy domestic ducks in Southern China in 2014. Our results show that the viruses grew efficiently in eggs and replicated systemically in chickens. They were completely lethal in chicken (100% mortality), and the mean death time (MDT) was 6 to 7 days post-inoculation (DPI). The viruses could transmit in chickens by naĂŻve contact. BLAST analysis revealed that their HA gene was most closely related to A/wild duck/Shangdong/628/2011 (H5N1), and their NA genes were most closely related to A/swine/Guangdong/K6/2010 (H6N6). The other genes had the highest identity with A/wild duck/Fujian/1/2011(H5N1). The results of phylogenetic analysis showed that their HA genes clustered into clade 2.3.4.4 of the H5N1 viruses and all genes derived from H5 were Mix-like or H6-like viruses. Thus, the new H5N6 viruses were reassortanted of H5N1 and H6N6 virus. Therefore, the circulation of the new H5N6 avian influenza viruses may become a threat to poultry and human health
Centrality dependence of spectra for identified hadrons in Au+Au and Cu+Cu collisions at GeV
The centrality dependence of transverse momentum spectra for identified
hadrons at midrapidity in Au+Au collisions at GeV is
systematically studied in a quark combination model. The
spectra of , , and in
different centrality bins and the nuclear modification factors () for
these hadrons are calculated. The centrality dependence of the average
collective transverse velocity for the hot and dense quark matter
is obtained in Au+Au collisions, and it is applied to a relative smaller Cu+Cu
collision system. The centrality dependence of spectra and
the for , and in Cu+Cu collisions at
GeV are well described. The results show that is only a function of the number of participants and it is
independent of the collision system.Comment: 7 pages, 6 figure
SUMOylation of Grb2 enhances the ERK activity by increasing its binding with Sos1
BACKGROUND: Grb2 (Growth factor receptor-bound protein 2) is a key adaptor protein in maintaining the ERK activity via linking Sos1 (Son of sevenless homolog 1) or other proteins to activated RTKs, such as EGFR. Currently, little knowledge is available concerning the post-translational modification (PTM) of Grb2 except for its phosphorylation. Since emerging evidences have highlighted the importance of SUMOylation (Small ubiquitin-related modifier), a reversible PTM, in modulating protein functions, we wondered if Grb2 could be SUMOylated and thereby influences its functions especially involved in the Ras/MEK/ERK pathway. METHODS: SUMOylation of Grb2 was analyzed with the in vivo SUMOylation assay using the Ni(2+)-NTA affinity pulldown and the in vitro E.coli-based SUMOylation assay. To test the ERK activity and cell transformation, the murine fibroblast cell line NIH/3T3 and the murine colon cancer cell line CMT-93 were used for the experiments including Grb2 knockdown, ectopic re-expression, cell transformation and migration. Immunoprecipitation (IP) was employed for seeking proteins that interact with SUMO modified Grb2. Xenograft tumor model in mice was conducted to verify that Grb2 SUMOylation regulated tumorigenesis in vivo. RESULTS: Grb2 can be SUMOylated by SUMO1 at lysine 56 (K(56)), which is located in the linker region between the N-terminal SH3 domain and the SH2 domain. Knockdown of Grb2 reduced the ERK activity and suppressed cell motility and tumorigenesis in vitro and in vivo, which were all rescued by stable ectopic re-expression of wild-type Grb2 but not the mutant Grb2(K56R). Furthermore, Grb2 SUMOylation at K(56) increased the formation of Grb2-Sos1 complex, which sequentially leads to the activation of Ras/MEK/MAPK pathway. CONCLUSIONS: Our results provide evidences that Grb2 is SUMOylated in vivo and this modification enhances ERK activities via increasing the formation of Grb2-Sos1 complex, and may consequently promote cell motility, transformation and tumorigenesis
The Resistance Phenotype and Molecular Epidemiology of Klebsiella pneumoniae in Bloodstream Infections in Shanghai, China, 2012–2015
Percutaneous closure of postinfarct muscular ventricular septal defects: A multicenter study in China
AbstractBackgroundSurgical repair is an effective method to treat ventricular septal defect (VSD) complicating acute myocardial infarction (AMI). However, the mortality rate remains high. This study was designed to assess the immediate and mid-term results of transcatheter closure of postinfarct muscular VSDs.MethodsData were retrospectively collected from 42 AMI patients who underwent attempted transcatheter VSD closure between 2008 and 2012 in seven heart centers of China.ResultsNine patients underwent emergent VSD closure in the acute phase (within two weeks from VSD) while the others underwent elective closure. The time between VSD occurrence and closure in emergency group and elective group was 7.7±2.3 days and 35±14.5 days, respectively (p<0.01). The percentage of procedure success in the emergency group and elective group was 77.8% (7/9) and 97% (32/33), respectively (p=0.048). The hospital mortality was higher for emergent closure in comparison to elective closure (66.7% vs. 6.1%, p<0.01). During a median follow-up of 25 months (0–58 months), two patients died at 8 and 29 months, respectively, and no serious complications occurred in other patients.ConclusionInterventional postinfarct VSD closure is a safe and effective approach that can be performed with a high procedural success rate, with favorable outcomes if it can be undertaken >14 days postinfarct
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