3 research outputs found
Study on Retrieval of Chlorophyll-a Concentration Based on Landsat OLI Imagery in the Haihe River, China
The optical complexity of urban waters makes the remote retrieval of chlorophyll-a (Chl-a) concentration a challenging task. In this study, Chl-a concentration was retrieved using reflectance data of Landsat OLI images. Chl-a concentration in the Haihe River of China was obtained using mathematical regression analysis (MRA) and an artificial neural network (ANN). A regression model was built based on an analysis of the spectral reflectance and water quality sampling data. Remote sensing inversion results of Chl-a concentration were obtained and analyzed based on a verification of the algorithm and application of the models to the images. The analysis results revealed that the two models satisfactorily reproduced the temporal variation based on the input variables. In particular, the ANN model showed better performance than the MRA model, which was reflected in its higher accuracy in the validation. This study demonstrated that Landsat Operational Land Imager (OLI) images are suitable for remote sensing monitoring of water quality and that they can produce high-accuracy inversion results
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Spatially organized cellular communities form the developing human heart.
The heart, which is the first organ to develop, is highly dependent on its form to function1,2. However, how diverse cardiac cell types spatially coordinate to create the complex morphological structures that are crucial for heart function remains unclear. Here we integrated single-cell RNA-sequencing with high-resolution multiplexed error-robust fluorescence in situ hybridization to resolve the identity of the cardiac cell types that develop the human heart. This approach also provided a spatial mapping of individual cells that enables illumination of their organization into cellular communities that form distinct cardiac structures. We discovered that many of these cardiac cell types further specified into subpopulations exclusive to specific communities, which support their specialization according to the cellular ecosystem and anatomical region. In particular, ventricular cardiomyocyte subpopulations displayed an unexpected complex laminar organization across the ventricular wall and formed, with other cell subpopulations, several cellular communities. Interrogating cell-cell interactions within these communities using in vivo conditional genetic mouse models and in vitro human pluripotent stem cell systems revealed multicellular signalling pathways that orchestrate the spatial organization of cardiac cell subpopulations during ventricular wall morphogenesis. These detailed findings into the cellular social interactions and specialization of cardiac cell types constructing and remodelling the human heart offer new insights into structural heart diseases and the engineering of complex multicellular tissues for human heart repair