DataSheet1_The prognostic value of preoperative D-dimer to albumin ratio for overall survival and progression-free survival in colorectal cancer.docx

Introduction: This study aimed to explore the predictive value of the D-dimer-to-albumin ratio (DAR) for progression-free survival (PFS) and overall survival (OS) in patients with colorectal cancer (CRC).Methods: The Kaplan-Meier method was used to plot survival curves for PFS and OS. Receiver operating characteristic (ROC) curve analysis was used to evaluate the predictive efficacy of the DAR for PFS and OS in patients with CRC. Cox proportional hazards regression analysis was used to analyze prognostic factors influencing outcomes. A nomogram based on the DAR was constructed to predict 1-, 3-, and 5-year prognoses of patients with CRC; its predictive ability was evaluated using the concordance index (C-index) and calibration curves. Additionally, the clinical utility of the DAR-based nomogram was validated using an internal randomized validation cohort.Results: A total of 1,339 patients with CRC who underwent surgery were enrolled. The optimal cut-off value for DAR was determined to be 3.320, dividing patients into low (Conclusion: Preoperative DAR is a promising biomarker for predicting PFS and OS among patients with CRC. The DAR-based prognostic prediction nomogram may serve as an effective tool for the comprehensive assessment of prognosis in patients with CRC.</p

DataSheet_1_The value of carcinoembryonic antigen stage in staging, prognosis, and management of colorectal cancer: results from two cohort studies.docx

BackgroundCombining the carcinoembryonic antigen (CEA) level (C stage) with TNM staging can provide a more comprehensive prognostic assessment of colorectal cancer (CRC). However, the clinical value of incorporating CEA status into the TNM staging system needs to be evaluated.MethodsWe used the SEER database (N = 49,350) and a retrospective cohort from China (N = 1,440). A normal CEA level was staged as C0 and an elevated CEA level was staged as C1. Restricted cubic spline analysis was used to examine the dose-response relationship between the CEA level and survival. The Kaplan-Meier method with the log-rank test was used to plot survival curves. Multivariable Cox proportional hazards regression models with forward stepwise variable selection were used to estimate the hazard ratios and 95% confidence intervals.ResultsPatients with C1 were more likely to have advanced disease than those with C0. CEA on a continuous scale was positively associated with mortality risk. Compared with patients with C0 stage, those with C1 stage had significantly lower survival rates. In the SEER dataset, C1 was independently associated with poor prognosis in patients with CRC, with an approximately 70% increased risk of mortality. Patients with C1 stage had significantly lower survival than those with C0 stage at all clinical stages. Incorporating the C stage into the TNM staging refined the prediction of prognosis of patients with CRC, with a gradual decline in prognosis from stage I C0 to stage IV C1. A similar pattern was observed in the present retrospective cohort study. At each lymph node stage, patients with C1 had significantly lower 5-year survival rates than patients with C0. Compared with lymph node positivity, CEA positivity may have a stronger correlation with a worse prognosis.ConclusionOur findings not only validated the independent prognostic significance of CEA in CRC but also demonstrated its enhanced prognostic value when combined with TNM staging. Our study provides evidence supporting the inclusion of C stage in the TNM staging system.</p

Three new antitumor annonaceous acetogenins from the seeds of <i>Annona squamosa</i>

<p>Two new annonaceous acetogenins squamocin P (2) and annosquatin III (3) and one new ACG precursor dieporeticenin B (1) along with five known precursors (4–8) were isolated from the seeds of <i>Annona squamosa</i>. Their structures were ascertained by chemical methods and various spectral evidences. These compounds showed inhibitory effects against three multidrug-resistant (MDR) cancer cell lines. Compound 2 and 3 displayed selective cytotoxicity against SMMC 7721/T (IC₅₀ 0.435 and 1.79 μM) and MCF-7/ADR (IC₅₀ values 3.34 and 4.04 μM).</p

Selective Production of Glycolic Acid from Cellulose Promoted by Acidic/Redox Polyoxometalates via Oxidative Hydrolysis

The direct conversion of cellulose to glycolic acid (GA) with a high yield of up to 75% is realized using acidic/redox polyoxometalates (POMs) as catalysts in a one-pot reaction. Analysis of the reaction pathway and mechanism for the three POMs H3PMo12O40 (H3PMo), H3PW12O40 (H3PW), and H5PMo10V2O40 (H5PMoV2) by density functional theory calculations and experiments shows that H3PMo is especially promising. Activation of O2 to •O2– and 1O2 via one-electron transfer assists the depolymerization process of cellulose by acidic/redox H3PMo. The reduced form [PMo10VIMo2VO39]5– plays a crucial role in GA production due to its high activity and ability to stabilize the intermediates of the retro-aldol reaction. H3PMo was furthermore complexed by the ionic liquid 1-(3-sulfonic group) propyl-3-methyl imidazolium (MIMPS), which enables easy recovery from the reaction solution due to temperature-responsive properties of the complexes. [MIMPS]H2PMo provides an outstanding GA selectivity of 61% under aerobic conditions and is comparable to the homogeneous H3PMo. Activity and selectivity to GA could be improved to 100 and 75%, respectively, by performing the reaction in the microwave at 190 °C for 2 min. The work deepens the insight on cellulosic biomass transformation over POMs by acidic/oxidative synergetic catalysis and contributes to the effort of designing highly active, selective, and multifunctional catalysts

The novel protein C9orf116 promotes rat liver cell line BRL-3A proliferation - Fig 7

<p>Effect of C9orf116 on mitotic entry (A) EdU incorporation assays. The Click-it reaction revealed EdU staining (red). Cell nuclei were stained with Hoechst 33342 (blue). (B) The percentage of EdU-positive cells was quantified. (C) The expression of p-H3 (Ser10) was evaluated by Western blot analysis in C9orf116 knockdown and overexpression BRL-3A cells compared to their control, respectively. (D) The expression of p-H3 (Ser10) was quantified. The experiments were performed three times and three replicates in each one. The data were shown as the means ± SD. Representative images were shown *<i>P</i><0.05, **<i>P</i><0.01.</p

Effect C9orf116 on the expression of cell proliferation related genes.

<p>(A) The expression of cell proliferation related genes in C9-siR2 group at the mRNA level by qRT-PCR analysis. (B) The expression of cell proliferation related genes in C9-siR2 group at the protein level by western blot analysis. (C) The expression of cell proliferation related genes in pCDH-C9 group at the mRNA level by qRT-PCR analysis. (D) The expression of cell proliferation related genes in pCDH-C9 group at the protein level by western blot analysis. The experiments were performed three times and three replicates in each one, the data were shown as the means ± SD. Representative images were shown *<i>P</i><0.05, **<i>P</i><0.01.</p

Effect of C9orf116 on BRL-3A cell proliferation.

<p>(A) The cell viability was assessed at 24, 48 and 72 h after transfected with siRNA and control by MTT assay. (B) The cell viability was assessed at 24, 48 and 72 h in C9orf116 over-expression cells and control by MTT assay. (C) Proliferation measurement by counting live cells in haemocytometer chamber after trypan blue staining, the number of live cells was counted at 24, 48 and 72 h after transfected with siRNA and control. (D) The number of live cells was counted at 24, 48 and 72 h in C9orf116 over-expression cells and control. NC, C9-siR2 represents the cells treated with negative siRNA, siR2-C9orf116, respectively; pCDH, pCDH-C9 represents the cells treated with Empty vector (pCDH), pCDH-C9orf116, respectively. The above results were presented as means ± SD. The experiments were performed three times and three replications per group. * Indicates <i>p</i> <0.05.</p

The primer sequences used in the RT-PCR.

<p>The primer sequences used in the RT-PCR.</p

The effect of C9orf116 siRNAs on the mRNA expression level of C9orf116.

<p>NC. Negative siRNA; siR1, siR2 and siR3 represented cells treated with C9orf116 siRNA 1, siRNA 2 and siRNA 3, respectively. The experiments were performed three times and three replicates in each one, the data were shown as the means ± SD, *<i>P</i><0.05, **<i>P</i><0.01.</p

The sequence of C9orf116 siRNAs.

<p>The sequence of C9orf116 siRNAs.</p