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Additional file 2: Figure S2. of Activation of P2X7 receptor and NLRP3 inflammasome assembly in hippocampal glial cells mediates chronic stress-induced depressive-like behaviors
There was no significant sexual difference in the mice model of depression induced by chronic unpredictable stress. (A) Experimental paradigm. Wild-type C57BL6/J (WT) male and female mice were exposed to CUS for 35Β days. Behavioral indicators were then assessed, including (B) immobility time in forced swimming test (FST) (interaction: F1,34β=β0.0003, pβ=β0.9857; stress: F1,34β=β26.51, pβ<β0.0001; sex: F1,34β=β0.4940, pβ=β0.4869), (C) the number of rearing in open-field test (OFT) (interaction: F1,34β=β0.01154, pβ=β0.9151; stress: F1,34β=β20.44, pβ<β0.0001; sex: F1,34β=β0.1414, pβ=β0.7092), (D) total distance in open-field test (OFT) (interaction: F1,34β=β0.03584, pβ=β0.8510; stress: F1,34β=β4.501, pβ=β0.0412; sex: F1,34β=β0.1341, pβ=β0.7165), (E) open-arm entrance percent in elevated plus maze test (EPM) (interaction: F1,34β=β0.1817, pβ=β0.6728; stress: F1,34β=β16.47, pβ=β0.0003; sex: F1,34β=β7.879, pβ=β0.0084), (F) open-arm time percent in elevated plus maze test (EPM) (interaction: F1,34β=β0.7491, pβ=β0.3932; stress: F1,34β=β5.100, pβ=β0.0309; sex: F1,34β=β0.2789, pβ=β0.6011) nβ=β8β12 per group, all data are expressed as the meanβΒ±βSEM. # pβ<β0.05, ## pβ<β0.01, ### pβ<β0.001, compared to male before CUS. *pβ<β0.05 and **pβ<β0.01, compared to female before CUS. (G) Experimental paradigm. Wild-type C57BL6/J (WT) and P2X7-null female mice were exposed to CUS for 35Β days. Behavioral indicators were then assessed, including (H) immobility time in forced swimming test (FST) (interaction: F1,23β=β1.038, pβ=β0.3188; stress: F1,23β=β8.155, pβ=β0.0089; genotype: F1,23β=β1.610, pβ=β0.2171), (I) the number of rearing in open-field test (OFT) (interaction: F1,23β=β3.690, pβ=β0.0672; stress: F1,23β=β3.929, pβ=β0.0595; genotype: F1,23β=β1.221, pβ=β0.2805), (J) total distance in open-field test (OFT) (interaction: F1,23β=β4.348, pβ=β0.0483; stress: F1,23β=β0.2596, pβ=β0.6153; genotype: F1,23β=β2.684, pβ=β0.1150), (K) open-arm entrance percent in elevated plus maze test (EPM) (interaction: F1,23β=β5.294, pβ=β0.0308; stress: F1,23β=β2.595, pβ=β0.1208; genotype: F1,23β=β1.976, pβ=β0.1732), and (L) open-arm time percent in elevated plus maze test (EPM) (interaction: F1,23β=β5.914, pβ=β0.0232; stress: F1,23β=β3.100, pβ=β0.0916; genotype: F1,23β=β3.463, pβ=β0.0756). nβ=β5β8 per group, all data are expressed as the meanβΒ±βSEM. # pβ<β0.05, ## pβ<β0.01, ### pβ<β0.001, compared to wild-type before CUS. pβ<β0.05, comparing genotypes. (TIF 9760 kb