A-6G and A-20C Polymorphisms in the Angiotensinogen Promoter and Hypertension Risk in Chinese: A Meta-Analysis

BACKGROUND: Numerous studies in Chinese populations have evaluated the association between the A-6G and A-20C polymorphisms in the promoter region of angiotensinogen gene and hypertension. However, the results remain conflicting. We carried out a meta-analysis for these associations. METHODS AND RESULTS: Case-control studies in Chinese and English publications were identified by searching the MEDLINE, EMBASE, CNKI, Wanfang, CBM, and VIP databases. The random-effects model was applied for dichotomous outcomes to combine the results of the individual studies. We finally selected 24 studies containing 5932 hypertensive patients and 5231 normotensive controls. Overall, we found significant association between the A-6G polymorphism and the decreased risk of hypertension in the dominant genetic model (AA+AG vs. GG: P=0.001, OR=0.71, 95%CI 0.57-0.87, P(heterogeneity)=0.96). The A-20C polymorphism was significantly associated with the increased risk for hypertension in the allele comparison (C vs. A: P=0.03, OR=1.14, 95%CI 1.02-1.27, P(heterogeneity)=0.92) and recessive genetic model (CC vs. CA+AA: P=0.005, OR=1.71, 95%CI 1.18-2.48, P(heterogeneity)=0.99). In the subgroup analysis by ethnicity, significant association was also found among Han Chinese for both A-6G and A-20C polymorphisms. A borderline significantly decreased risk of hypertension between A-6G and Chinese Mongolian was seen in the allele comparison (A vs. G: P=0.05, OR=0.79, 95%CI 0.62-1.00, P(heterogeneity)=0.84). CONCLUSION: Our meta-analysis indicated significant association between angiotensinogen promoter polymorphisms and hypertension in the Chinese populations, especially in Han Chinese

Association Study of the β2-Adrenergic Receptor Gene Polymorphisms and Hypertension in the Northern Han Chinese

Background: The beta 2-adrenergic receptor (ADRB2) gene has been widely researched as a candidate gene for essential hypertension (EH), but no consensus has been reached in different ethnicities. The aim of the present study was to evaluate the possible association between the ADRB2 gene polymorphisms and the EH risk in the Northern Han Chinese population. Methodology/Principal Findings: This study included 747 hypertensive subjects and 390 healthy volunteers as control subjects in the Northern Han Chinese. Genotyping was performed to identify the C-47T, A46G and C79G polymorphisms of the ADRB2 gene. G allelic frequency of A46G polymorphism was significantly higher in hypertensive subjects (P = 0.011, OR = 1.287, 95% CI [1.059-1.565]) than that in controls. Significant association could also be found in dominant genetic model (GG+AG vs. AA, P = 0.006, OR = 1.497, 95% CI [1.121-1.998]), in homozygote comparison (GG vs. AA, P = 0.025, OR = 1.568, 95% CI [1.059-2.322]), and in additive genetic model (GG vs. AG vs. AA, P = 0.012, OR = 1.282, 95% CI [1.056-1.555]). Subgroup analyses performed by gender suggested that this association could be found in male, but not in female. Stratification analyses by obesity showed that A46G polymorphism was related to the prevalence of hypertension in the obese population (GG vs. AG vs. AA, P<0.001, OR = 1.645, 95% CI [1.258-2.151]). Significant interaction was found between A46G genotypes and body mass index on EH risk. No significant association could be found between C-47T or C79G polymorphism and EH risk. Linkage disequilibrium was detected between the C-47T, A46G and C79G polymorphisms. Haplotype analyses observed that the T-47-A46-C79 haplotype was a protective haplotype for EH, while the T-47-G46-C79 haplotype increased the risk. Conclusions/Significances: We revealed that the ADRB2 A46G polymorphism might increase the risk for EH in the Northern Han Chinese population.Multidisciplinary SciencesSCI(E)7ARTICLE4null

Petrophysical facies characteristics and classification evaluation of Dongying Formation of Nanpu No. 4 structure

Strong diagenesis and reservoir heterogeneity as well as complex pore structure in the late stage of No. 4 structure in Nanpu sag make assessment of the petrophysical characteristics of its high-quality reservoir and evaluation of its effectiveness difficult. To address this, the sedimentary, diagenetic, and pore structure characteristics of the reservoir were comprehensively studied using core thin section analysis, scanning electron microscopy, X-ray diffraction, capillary analysis, and logging and oil tests. The results showed that the sedimentary facies of the Ed2 and Ed3 of the study area were mainly braided river delta types, and the sedimentary microfacies mainly developed in an underwater distributary channel, interdistributary bay, and mouth bar. Diagenetic facies can be divided into four types based on diagenesis and mineral types: weak dissolution facies, clay mineral filling facies, carbonate cementation facies, and compacted dense facies. The pore structure facies can be divided into four types based on the reservoir physical properties and mercury injection, I: macropore coarse throat type, II: macropore medium throat type, III: mesopore thin throat type, and IV: micropore throat type. Based on the superimposed cluster analysis of sedimentary, diagenesis, and pore structure, the reservoir petrophysical facies can be divided into PF1-PF4, and the corresponding quantitative classification and evaluation criteria can be established. PF1 is an advantageous reservoir with high oil, gas, and water productivity; PF2 is an oil-bearing reservoir with average productivity; PF3 is a poor reservoir with low productivity after reservoir reconstruction; and PF4 is an invalid reservoir. The quantitative classification and evaluation criteria of petrophysical facies are established by logging response rules, which provide technical support and a solid theoretical basis for the evaluation of reservoir effectiveness, superior reservoir prediction, and subsequent ongoing development in the study area

Side of Limb-Onset Predicts Laterality of Gray Matter Loss in Amyotrophic Lateral Sclerosis

Conflicting findings have been reported regarding the lateralized brain abnormality in patients with amyotrophic lateral sclerosis (ALS). In this study, we aimed to investigate the probable lateralization of gray matter (GM) atrophy in ALS patients. We focused on the relationship between the asymmetry in decreased GM volume and the side of disease onset in patients with limb-onset. Structural imaging evaluation of normalized atrophy (SIENAX) and voxel-based morphometry (VBM) were used to assess differences in global and local brain regions in patients with heterogeneous body onset and subgroups with different side of limb-onset. We found global brain atrophy and GM losses in the frontal and parietal areas in each patient group as well as left predominant GM losses in the total cohort. The intriguing findings in subgroup analyses demonstrated that the motor cortex in the contralateral hemisphere of the initially involved limb was most affected. We also found that regional brain atrophy was related to disease progression rate. Our observations suggested that side of limb-onset can predict laterality of GM loss in ALS patients and disease progression correlates with the extent of cortical abnormality

Sirtuin 2 regulates neutrophil functions through NAD+ synthesis pathway in virus infection

Summary: Neutrophils play an important role in antiviral immunity, but the underlying mechanisms remain unclear. Here, we found that SIRT2 deficiency inhibited the infiltration of neutrophils, as well as the secretion of inflammatory cytokines and the formation of neutrophil extracellular traps (NETs), ameliorating disease symptoms during acute respiratory virus infection. Mechanistically, SIRT2 deficiency upregulates quinolinic acid (QA)-producing enzyme 3-hydroxyanthranilate oxygenase (3-HAO) and leads to expression of quinolinate phosphoribosyltransferase (QPRT), which promotes the synthesis of QA for NAD+ and limits viral infection when de novo NAD+ synthesis is blocked. Tryptophan-2,3-oxygenase expressed in epithelial cells metabolizes tryptophan to produce kynurenine and 3-hydroxyaminobenzoic acid, which is a source of intracellular QA in neutrophils. Thus, our findings reveal a previously unrecognized QPRT-mediated switch in NAD+ metabolism by exploiting neutrophil-derived QA as an alternative source of replenishing intracellular NAD+ pools induced by SIRT2 to regulate neutrophil functions during virus infection, with implications for future immunotherapy approaches

Motor network plasticity and low-frequency oscillations abnormalities in patients with brain gliomas: a functional MRI study.

Brain plasticity is often associated with the process of slow-growing tumor formation, which remodels neural organization and optimizes brain network function. In this study, we aimed to investigate whether motor function plasticity would display deficits in patients with slow-growing brain tumors located in or near motor areas, but who were without motor neurological deficits. We used resting-state functional magnetic resonance imaging to probe motor networks in 15 patients with histopathologically confirmed brain gliomas and 15 age-matched healthy controls. All subjects performed a motor task to help identify individual motor activity in the bilateral primary motor cortex (PMC) and supplementary motor area (SMA). Frequency-based analysis at three different frequencies was then used to investigate possible alterations in the power spectral density (PSD) of low-frequency oscillations. For each group, the average PSD was determined for each brain region and a nonparametric test was performed to determine the difference in power between the two groups. Significantly reduced inter-hemispheric functional connectivity between the left and right PMC was observed in patients compared with controls (P<0.05). We also found significantly decreased PSD in patients compared to that in controls, in all three frequency bands (low: 0.01-0.02 Hz; middle: 0.02-0.06 Hz; and high: 0.06-0.1 Hz), at three key motor regions. These findings suggest that in asymptomatic patients with brain tumors located in eloquent regions, inter-hemispheric connection may be more vulnerable. A comparison of the two approaches indicated that power spectral analysis is more sensitive than functional connectivity analysis for identifying the neurological abnormalities underlying motor function plasticity induced by slow-growing tumors

Mapping high-resolution forest aboveground biomass of China using multisource remote sensing data

Forest aboveground biomass (AGB) estimation is crucial for carbon cycle studies and climate change mitigation actions. However, because of limitations in timely and reliable forestry surveys and high-resolution remote sensing data, producing a fine resolution and spatial continuous forest AGB map of China is challenging. Here, we combined 4789 ground-truth AGB measurements and multisource remote sensing data such as a recently released forest canopy-height product, optical spectral indexes, topographic data, climatological data, and soil properties to train a random forest regression model for forest AGB estimation of China at 30-m resolution. The accuracy of the estimated AGB can yield R2 = 0.67 and RMSE = 70.71 Mg/ha. The nationwide estimates show that the average forest AGB and total forest carbon storage were 97.57 ± 23.85 Mg/ha and 11.06 Pg C for the year 2019, respectively. The value of AGB uncertainty ranges from 0.68 Mg/ha to 37.80 Mg/ha, and the average AGB uncertainty was 4.32 ± 1.75 Mg/ha. The forest AGB estimates of China in this study correspond reasonably well with the AGB estimates derived from the forestry and grassland statistical yearbook at the provincial level (R2 = 0.61, RMSE = 30.15 Mg/ha). In addition, we found that previous AGB products generally underestimate the forest AGB compared with our estimated AGB at the pixel-level and ground-truth AGB measurements. The high-resolution forest AGB map provides an important alternative data source for forest carbon cycle studies and can be used as a baseline map for forest management and conservation practices

E-selectin gene polymorphisms are associated with essential hypertension: a case-control pilot study in a Chinese population

Abstract Background Genetic variation is thought to contribute to the etiology of hypertension, and E-selectin is a candidate essential hypertension-associated gene. This study thus sought to investigate possible genetic associations between the T1880C, C602A and T1559C polymorphisms of E-selectin and essential hypertension. Methods Hypertensive patients (n = 490) and healthy normotensive subjects (n = 495) were screened for the genotypes T1880C, C602A and T1559C using real-time quantitative polymerase chain reaction after DNA extraction to identify representative variations in the E-selectin gene. The associations between genotypes and alleles of the three mutations and essential hypertension were then analyzed using a case-control study. Results Hypertensive patients and normotensive subjects were significantly different with respect to the genotypes CC, CA and AA (P = 0.005) and the C-allele frequency of C602A (P = 0.001). A comparison of dominant versus recessive models also revealed significant differences between the two groups (P = 0.004 and P = 0.02). When subgrouped by gender, these indexes differed significantly between normotensive and essential hypertensive males, but not in females. The additive model of the T1559C genotype did not differ between essential hypertensive and normotensive groups overall (P = 0.39), but it was different between hypertensive and normotensive males (P = 0.046) and females (P = 0.045). The CC + TC versus TT frequency of T1559C was also different in the recessive model of male hypertensive and normotensive groups (P = 0.02). Further analysis showed that C602A and T1559C were significantly associated with hypertension (C602A: OR = 7.58, 95%CI = 1.53-11.97, P Conclusion C602A and T1559C may be independent risk factors for essential hypertension in the Chinese population, whereas T1880C is not.</p

Mapping high-resolution forest aboveground biomass of China using multisource remote sensing data

Forest aboveground biomass (AGB) estimation is crucial for carbon cycle studies and climate change mitigation actions. However, because of limitations in timely and reliable forestry surveys and high-resolution remote sensing data, producing a fine resolution and spatial continuous forest AGB map of China is challenging. Here, we combined 4789 ground-truth AGB measurements and multisource remote sensing data such as a recently released forest canopy-height product, optical spectral indexes, topographic data, climatological data, and soil properties to train a random forest regression model for forest AGB estimation of China at 30-m resolution. The accuracy of the estimated AGB can yield R2 = 0.67 and RMSE = 70.71 Mg/ha. The nationwide estimates show that the average forest AGB and total forest carbon storage were 97.57 ± 23.85 Mg/ha and 11.06 Pg C for the year 2019, respectively. The value of AGB uncertainty ranges from 0.68 Mg/ha to 37.80 Mg/ha, and the average AGB uncertainty was 4.32 ± 1.75 Mg/ha. The forest AGB estimates of China in this study correspond reasonably well with the AGB estimates derived from the forestry and grassland statistical yearbook at the provincial level (R2 = 0.61, RMSE = 30.15 Mg/ha). In addition, we found that previous AGB products generally underestimate the forest AGB compared with our estimated AGB at the pixel-level and ground-truth AGB measurements. The high-resolution forest AGB map provides an important alternative data source for forest carbon cycle studies and can be used as a baseline map for forest management and conservation practices.</p