2,4-Dihydr­oxy-N′-(2-hydr­oxy-4-methoxy­benzyl­idene)benzohydrazide

In the title compound, C15H14N2O5, the dihedral angle between the two benzene rings is 4.3 (3)° and the mol­ecule adopts an E configuration with respect to the C=N bond. Intra­molecular O—H⋯N and N—H⋯O hydrogen bonds are observed. In the crystal structure, the mol­ecules are linked through inter­molecular N—H⋯O and O—H⋯O hydrogen bonds to form layers parallel to the ac plane

N′-(2-Chloro­benzyl­idene)-2-hydr­oxy-3-methyl­benzohydrazide

In the title compound, C15H13ClN2O2, the dihedral angle between the two benzene rings is 3.4 (5)° and the mol­ecule adopts an E configuration with respect to the C=N bond. There is an intra­molecular O—H⋯O hydrogen bond in the mol­ecule, which generates an S(6) loop. In the crystal structure, mol­ecules are linked through inter­molecular N—H⋯O hydrogen bonds, forming C(4) chains running along the a axis

Characterization of a female-specific protein from the wild silkworm Actias selene

Abstract In Actias selene (Hubner) (Lepidoptera Saturniidae) there is at least one female specific protein limited to the fat body. Sodium dodecyl sulfate polyacrylamide gel electrophoresis performed on the fat body proteins from male and female pupas of A. selene and a female-specific protein with a molecular weight of about 24 kDa was revealed. This protein was purified for determination of the Nterminal amino acid sequence. Conventional polymerase chain reaction (PCR) and rapid amplification of cDNA ends (RACE) PCR were also carried out to clone the cDNA encoding 24 kDa protein (As-24K) based on the amino acid residues. The As-24K cDNA consists of 832 bp and the amino acid sequence shares 71% similarity with the 24 kDa proteins from Antheraea pernyi (GuerinMeneville) and Antheraea yamamai (Guerin-Meneville) (Lepidoptera Saturniidae). In female pupa western blotting using antiserum against As-24K revealed that 24 kDa protein was restricted to the fat body. It was not found in any other pupal tissues

N′-(2,5-Dihydroxy­benzyl­idene)-2-hydr­oxy-3-methyl­benzohydrazide

In the title compound, C15H14N2O4, the dihedral angle between the two benzene rings is 4.1 (2)°. The mol­ecule adopts an E configuration with respect to the C=N bond. There are intra­molecular O—H⋯N and O—H⋯O hydrogen bonds in the mol­ecule. In the crystal structure, mol­ecules are linked through inter­molecular N—H⋯O and O—H⋯O hydrogen bonds, forming chains running along the c axis

2-Hydr­oxy-N′-(2-hydr­oxy-3-meth­oxy-5-nitro­benzyl­idene)-3-methyl­benzo­hydrazide

In the title compound, C16H15N3O6, the dihedral angle between the two benzene rings is 0.9 (2)°. The mol­ecule adopts an E configuration with respect to the C=N bond. There are intra­molecular O—H⋯N and O—H⋯O hydrogen bonds in the mol­ecule. In the crystal structure, mol­ecules are linked through inter­molecular N—H⋯O hydrogen bonds to form chains running along the c axis

Surface skyrmions and dual topological Hall effect in antiferromagnetic topological insulator EuCd2_2As2_2

In this work, we synthesized single crystal of EuCd$_2$As$_2$, which exhibits A-type antiferromagnetic (AFM) order with in-plane spin orientation below $T_N$ = 9.5~K.Optical spectroscopy and transport measurements suggest its topological insulator (TI) nature with an insulating gap around 0.1eV. Remarkably, a dual topological Hall resistivity that exhibits same magnitude but opposite signs in the positive to negative and negative to positive magnetic field hysteresis branches emerges below 20~K. With magnetic force microscopy (MFM) images and numerical simulations, we attribute the dual topological Hall effect to the N\'{e}el-type skyrmions stabilized by the interactions between topological surface states and magnetism, and the sign reversal in different hysteresis branches indicates potential coexistence of skyrmions and antiskyrmions. Our work uncovers a unique two-dimensional (2D) magnetism on the surface of intrinsic AFM TI, providing a promising platform for novel topological quantum states and AFM spintronic applications.Comment: 7 pages, 3 figure

MiR-143 acts as a tumor suppressor by targeting N-RAS and enhances temozolomide-induced apoptosis in glioma.

Therapeutic applications of microRNAs (miRNAs) in RAS-driven glioma were valuable, but their specific roles and functions have yet to be fully elucidated. Here, we firstly report that miR-143 directly targets the neuroblastoma RAS viral oncogene homolog (N-RAS) and functions as a tumor-suppressor in glioma. Overexpression of miR-143 decreased the expression of N-RAS, inhibited PI3K/AKT, MAPK/ERK signaling, and attenuated the accumulation of p65 in nucleus of glioma cells. In human clinical specimens, miR-143 was downregulated where an adverse with N-RAS expression was observed. Furthermore, overexpression of miR-143 decreased glioma cell migration, invasion, tube formation and slowed tumor growth and angiogenesis in a manner associated with N-RAS downregulation in vitro and in vivo. Finally, miR-143 also sensitizes glioma cells to temozolomide (TMZ),the first-line drug for glioma treatment. Taken together, for the first time, our results demonstrate that miR-143 plays a significant role in inactivating the RAS signaling pathway through the inhibition of N-RAS, which may provide a novel therapeutic strategy for treatment of glioma and other RAS-driven cancers

Low Mannose Binding Lectin, but Not L-Ficolin, Is Associated With Spontaneous Clearance of Hepatitis C Virus After Infection

Some individuals can spontaneously clear the hepatitis C virus (HCV) after infection, whereas others develop a chronic infection. The exact mechanism of this phenomenon is unknown. We aimed to evaluate the association of plasma levels of MBL, L-ficolin, and cytokines with outcome of HCV infections in two groups of patients who cleared HCV spontaneously (CHS), and who developed chronic HCV infections (CHC). Altogether, 86 patients and 183 healthy controls were included. Of 86 patients, 36 had CHS and 50 had CHC. Concentrations of plasma MBL and L-ficolin were measured in patients and controls. Twenty plasma cytokines and adhesion molecules, including GM-CSF, ICAM-1, IFN-gamma, IFN-alpha, IL-1 alpha, IL-1 beta, IL-10, IL-12p70, IL-13, IL-17A, IL-4, IL-8, IP-10, MCP-1, IL-6, MIP-1 alpha, MIP-1 beta, sE-Selectin, sP-Selectin, and TNF-alpha, were determined in all patients and randomly selected 45 controls. The level of MBL was significantly lower in subjects with CHS than in healthy controls (median: 293.10 vs. 482.64 ng/ml, p = 0.008), whereas the level of MBL was significantly higher in patients with CHC than in controls (median: 681.32 vs. 482.64 ng/ml, p = 0.001). No such differences in plasma L-ficolin were observed. Plasma levels of all cytokines and adhesion molecules, except ICAM-1, were significantly higher in patients than in controls. Moreover, patients with CHC had significantly higher levels of IFN-gamma, IFN-alpha, IL-1 alpha, IL-10, IL-13, IL-4, IL-6, and TNF-alpha than those with CHS. These findings implicate that lower levels of plasma MBL, together with lower levels of above mentioned cytokines may play a part in virus clearance of HCV infection

A simulation study on the measurement of D0-D0bar mixing parameter y at BES-III

We established a method on measuring the \dzdzb mixing parameter $y$ for BESIII experiment at the BEPCII $e^+e^-$ collider. In this method, the doubly tagged $\psi(3770) \to D^0 \overline{D^0}$ events, with one $D$ decays to CP-eigenstates and the other $D$ decays semileptonically, are used to reconstruct the signals. Since this analysis requires good $e/\pi$ separation, a likelihood approach, which combines the $dE/dx$, time of flight and the electromagnetic shower detectors information, is used for particle identification. We estimate the sensitivity of the measurement of $y$ to be 0.007 based on a $20fb^{-1}$ fully simulated MC sample.Comment: 6 pages, 7 figure