Marine spatial planning in reality: introduction to case studies and discussion of findings

This paper explores the realities of marine spatial planning (MSP'ing), drawing on 12 case studies around Europe, employing a structured qualitative empirical approach. The findings indicate that (1) MSP'ing is often focused on achieving specific sectoral objectives, related to nationally important strategic priorities, and might better be termed 'strategic sectoral planning'. (2) MSP'ing processes tend to be complex, fragmented and emergent on an ad hoc basis, rather than cyclical, adaptive and prescribed on an a priori basis. (3) Top-down processes tend to dominate, more participative platforms tending to be 'disconnected by design' from executive decision-making. (4) Blue growth is the dominant overall priority, often aligned with strategic sectoral priorities, despite growing indications that the target for Good Environmental Status (GES) by 2020 is unlikely to be met. This is consistent with growing concerns about the tensions between the Marine Strategy Framework Directive and the Directive Establishing a Framework for Maritime Spatial Planning. It is concluded that the realities of how MSP'ing is working contrast with widely recognised concepts and ideals as to how MSP'ing should work, as integrated-use MSP'ing based on political expedience and blue growth priorities is diverging from and potentially competing with ecosystem-based MSP'ing, including marine protected area networks, based on GES priorities. It is argued that a more empirical approach should be taken to MSP'ing research, whereby conceptual approaches which integrate sustainable blue growth and GES co-evolve with marine spatial planning practices through critical analyses of whether the realities of MSP'ing are consistent with these concepts

A standing-wave thermoacoustic engine driven by liquid nitrogen

Thermoacoustic oscillation at cryogenic temperatures, such as Taconis oscillation, has been typically suppressed in the former studies, and few efforts have been made to enhance it. We proposed a standing-wave thermoacoustic engine (TE) driven by liquid cryogens instead of the conventional heat to enhance the thermoacoustic effects and utilize the cold energy. Experimental and theoretical work has been performed on a self-made standingwave TE to demonstrate the feasibility and the operating characteristics of the engine driven by the liquid nitrogen. Experiments show that with nitrogen at 0.5 MPa as a working gas, a pressure ratio of 1.21 is obtained on the TE driven by liquid nitrogen with a much lower temperature difference along the stack compared to that of the conventional TE. The onset temperature difference decreases by 28.9% with helium at 0.63 MPa as a working gas, compared to that of the conventional TE. This study verifies the feasibility of enhancing the thermoacoustic oscillation at cryogenic temperatures. The TEs driven by liquid cryogens such as liquid nitrogen and liquefied nature gas (LNG), may be an alternative for recovering the cold energy

Distributed Scheduling of Recording Tasks with Interconnected Servers

We consider a system with multiple interconnected video servers storing TV programs that are received through satellite antennas. Users, equipped with set-top boxes, submit requests for TV programs, to each of which they assign a utility value according to their preferences. We develop a distributed scheduling algorithm that selects the programs to be recorded and the servers to store them, so that a high total utility is generated to the users' population. Our scheduling algorithm is based on the programs' broadcasting information, the users' preferences, the constraints regarding the capabilities of simultaneous recordings and storage, and the system's topology. In fact, servers belonging to the same cluster co-operate in order to attain increased e#ciency by exchanging content through streaming or replication. The e#cient performance of our scheduling algorithm is shown by means of experiments. The algorithm constitutes a practically applicable solution, already implemented and integrated in the testbed of the IST project UP-TV

CSF and Brain Structural Imaging Markers of the Alzheimer's Pathological Cascade

10.1371/journal.pone.0047406PLoS ONE712

PKCε-dependent potentiation of TTX-resistant Nav1.8 current by neurokinin-1 receptor activation in rat dorsal root ganglion neurons

<p>Abstract</p> <p>Background</p> <p>Substance P (SP), which mainly exists in a subtype of small-diameter dorsal root ganglion (DRG) neurons, is an important signal molecule in pain processing in the spinal cord. Our previous results have proved the expression of SP receptor neurokinin-1 (NK-1) on DRG neurons and its interaction with transient receptor potential vanilloid 1 (TRPV1) receptor.</p> <p>Results</p> <p>In this study we investigated the effect of NK-1 receptor agonist on Na_v1.8, a tetrodotoxin (TTX)-resistant sodium channel, in rat small-diameter DRG neurons employing whole-cell patch clamp recordings. NK-1 agonist [Sar⁹, Met(O₂)¹¹]-substance P (Sar-SP) significantly enhanced the Na_v1.8 currents in a subgroup of small-diameter DRG neurons under both the normal and inflammatory situation, and the enhancement was blocked by NK-1 antagonist Win51708 and protein kinase C (PKC) inhibitor bisindolylmaleimide (BIM), but not the protein kinase A (PKA) inhibitor H89. In particular, the inhibitor of PKCε, a PKC isoform, completely blocked this effect. Under current clamp model, Sar-SP reduced the amount of current required to evoke action potentials and increased the firing rate in a subgroup of DRG neurons.</p> <p>Conclusion</p> <p>These data suggest that activation of NK-1 receptor potentiates Na_v1.8 sodium current via PKCε-dependent signaling pathway, probably participating in the generation of inflammatory hyperalgesia.</p

Numerical simulation on onset characteristics of traveling-wave thermoacoustic engines based on a time-domain network model

Onset characteristics of thermoacoustic engines are of great importance for understanding the internal working mechanisms of thermoacoustic conversion. A one-dimensional time-domain network model for predicting the onset characteristics of traveling-wave thermoacoustic engines with helium as working gas is built. The acoustic resistance, inertance, compliance, and thermal-relaxation effects of all the acoustic components are included. The viscous and heat transfer terms in the time-domain governing equations of the acoustic tubes and the heat exchangers are deduced from the frequency-domain linear thermoacoustic theory. Combining the time-domain governing equations of the regenerator, numerical simulations of the whole onset process are then conducted in a wide operating condition range. The complete dynamic pressure wave evolution processes are simulated successfully. It is shown that a steady standing-wave acoustic field forms in almost all parts of the traveling-wave thermoacoustic engine except for the regenerator area. Onset temperature, operating frequency, and quality factor are calculated with a relatively high accuracy. The thermal relaxation effects in the regenerator are found to have a remarkable impact on the onset characteristics, especially at high mean pressures. It is also shown that the experimental damping temperature is closer to the calculated onset temperature than the experimental onset temperature. Furthermore, the reasonable distributions of the pressure and volume flow rate and the phase relationship between them in the whole system are obtained and analyzed

Distinct genomic routes underlie transitions to specialised symbiotic lifestyles in deep-sea annelid worms.

Bacterial symbioses allow annelids to colonise extreme ecological niches, such as hydrothermal vents and whale falls. Yet, the genetic principles sustaining these symbioses remain unclear. Here, we show that different genomic adaptations underpin the symbioses of phylogenetically related annelids with distinct nutritional strategies. Genome compaction and extensive gene losses distinguish the heterotrophic symbiosis of the bone-eating worm Osedax frankpressi from the chemoautotrophic symbiosis of deep-sea Vestimentifera. Osedax's endosymbionts complement many of the host's metabolic deficiencies, including the loss of pathways to recycle nitrogen and synthesise some amino acids. Osedax's endosymbionts possess the glyoxylate cycle, which could allow more efficient catabolism of bone-derived nutrients and the production of carbohydrates from fatty acids. Unlike in most Vestimentifera, innate immunity genes are reduced in O. frankpressi, which, however, has an expansion of matrix metalloproteases to digest collagen. Our study supports that distinct nutritional interactions influence host genome evolution differently in highly specialised symbioses

Novel molecular approach to define pest species status and tritrophic interactions from historical Bemisia specimens

Museum specimens represent valuable genomic resources for understanding host-endosymbiont/parasitoid evolutionary relationships, resolving species complexes and nomenclatural problems. However, museum collections suffer DNA degradation, making them challenging for molecular-based studies. Here, the mitogenomes of a single 1912 Sri Lankan Bemisia emiliae cotype puparium, and of a 1942 Japanese Bemisia puparium are characterised using a Next-Generation Sequencing approach. Whiteflies are small sap-sucking insects including B. tabaci pest species complex. Bemisia emiliae’s draft mitogenome showed a high degree of homology with published B. tabaci mitogenomes, and exhibited 98–100% partial mitochondrial DNA Cytochrome Oxidase I (mtCOI) gene identity with the B. tabaci species known as Asia II-7. The partial mtCOI gene of the Japanese specimen shared 99% sequence identity with the Bemisia ‘JpL’ genetic group. Metagenomic analysis identified bacterial sequences in both Bemisia specimens, while hymenopteran sequences were also identified in the Japanese Bemisia puparium, including complete mtCOI and rRNA genes, and various partial mtDNA genes. At 88–90% mtCOI sequence identity to Aphelinidae wasps, we concluded that the 1942 Bemisia nymph was parasitized by an Eretmocerus parasitoid wasp. Our approach enables the characterisation of genomes and associated metagenomic communities of museum specimens using 1.5 ng gDNA, and to infer historical tritrophic relationships in Bemisia whiteflies.© The Author(s) 2017. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. The attached file is the published pdf

Amino Terminal Domains of the NMDA Receptor Are Organized as Local Heterodimers

The N-methyl-D-aspartate (NMDA) receptor, an obligate heterotetrameric assembly organized as a dimer of dimers, is typically composed of two glycine-binding GluN1 subunits and two glutamate-binding GluN2 subunits. Despite the crucial role that the NMDA receptor plays in the nervous system, the specific arrangement of subunits within the dimer-of-dimer assemblage is not conclusively known. Here we studied the organization of the amino terminal domain (ATD) of the rat GluN1/GluN2A and GluN1/GluN2B NMDA receptors by cysteine-directed, disulfide bond-mediated cross-linking. We found that GluN1 ATDs and GluN2 ATDs spontaneously formed disulfide bond-mediated dimers after introducing cysteines into the L1 interface of GluN2A or GluN2B ATD. The formation of dimer could be prevented by knocking out endogenous cysteines located near the L1 interface of GluN1. These results indicate that GluN1 and GluN2 ATDs form local heterodimers through the interactions in the L1-L1 interface and further demonstrate a dimer-of-heterodimer arrangement in GluN1/GluN2A and GluN1/GluN2B NMDA receptors