1 research outputs found
Fe-Porphyrin-Based Covalent Organic Framework As a Novel Peroxidase Mimic for a One-Pot Glucose Colorimetric Assay
Covalent
organic frameworks (COFs) have recently emerged as very
fascinating porous polymers due to their attractive design synthesis
and various applications. However, the catalytic application of COF
materials as enzymatic mimics remains largely unexplored. In this
work, the Fe-porphyrin-based covalent organic framework (Fe-COF) has
been successfully synthesized through a facile postsynthetic strategy
for the first time. In the presence of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), the Fe-COF can catalyze a chromogenic substrate (3,3′,5,5′-tetramethylbenzidine
(TMB)) to produce color, and this just goes to show that it has an
inner peroxidase-like activity. Moreover, the kinetic studies indicate
that the Fe-COF nanomaterial has a higher affinity toward both the
substrate H<sub>2</sub>O<sub>2</sub> and TMB than the natural enzyme,
horseradish peroxidase (HRP). Under the optimized conditions, the
Fe-COF nanomaterial was applied in a colorimetric sensor for the sensitive
detection of H<sub>2</sub>O<sub>2</sub>. The detection range was from
7 to 500 μM, and the detection limit was 1.1 μM. Furthermore,
the combination of the Fe-COF with glucose oxidase (GOx) can be implemented
to measure glucose by a one-pot method, and the obtained detection
range was from 5 to 350 μM; the detection limit was 1.0 μM.
It was proved that the sensor can be successfully used to detect the
concentration of glucose in human serum samples. As a peroxidase mimic,
the Fe-COF exhibits the advantages of easy preparation, good stability,
and ultrahigh catalytic efficiency. We believed that the proposed
method in this work would facilitate the applications of COF-based
composites as enzymatic mimics in biomedical fields