One-Step 18

Positron emission tomography (PET) imaging is a useful method to evaluate in situ estrogen receptor (ER) status for the early diagnosis of breast cancer and optimization of the appropriate treatment strategy. The 18F-labeled estradiol derivative has been successfully used to clinically assess the ER level of breast cancer. In order to simplify the radiosynthesis process, one-step 18F-19F isotope exchange reaction was employed for the 18F-fluorination of the tracer of [18F]AmBF3-TEG-ES. The radiotracer was obtained with the radiochemical yield (RCY) of ~61% and the radiochemical purity (RCP) of >98% within 40 min. Cell uptake and blocking assays indicated that the tracer could selectively accumulate in the ER-positive human breast cancer cell lines MCF-7 and T47D. In vivo PET imaging on the MCF-7 tumor-bearing mice showed relatively high tumor uptake (1.4~2.3 %D/g) and tumor/muscle uptake ratio (4~6). These results indicated that the tracer is a promising PET imaging agent for ER-positive breast cancers

Polymorphisms of XRCC4 are involved in reduced colorectal cancer risk in Chinese schizophrenia patients

<p>Abstract</p> <p>Background</p> <p>Genetic factors related to the regulation of apoptosis in schizophrenia patients may be involved in a reduced vulnerability to cancer. XRCC4 is one of the potential candidate genes associated with schizophrenia which might induce colorectal cancer resistance.</p> <p>Methods</p> <p>To examine the genetic association between colorectal cancer and schizophrenia, we analyzed five SNPs (rs6452526, rs2662238, rs963248, rs35268, rs2386275) covering ~205.7 kb in the region of XRCC4.</p> <p>Results</p> <p>We observed that two of the five genetic polymorphisms showed statistically significant differences between 312 colorectal cancer subjects without schizophrenia and 270 schizophrenia subjects (rs6452536, p = 0.004, OR 0.61, 95% CI 0.44-0.86; rs35268, p = 0.028, OR 1.54, 95% CI 1.05-2.26). Moreover, the haplotype which combined all five markers was the most significant, giving a global <it>p </it>= 0.0005.</p> <p>Conclusions</p> <p>Our data firstly indicate that XRCC4 may be a potential protective gene towards schizophrenia, conferring reduced susceptibility to colorectal cancer in the Han Chinese population.</p

Theoretical study on hydrolytic step in biotransformation of β-hexachlorocyclohexane degraded by haloalkane dehalogenase LinB

The hydrolytic process of LinB-catalyzed biotransformation of a notorious contaminant β-HCH was investigated in atomistic detail with a combined quantum mechanics/molecular mechanics approach. The Boltzmann-weighted averaging method amended by disproportionate effect analysis was showed to capture the fluctuation of a single molecule enzyme reaction. With the potential barriers of 18.7 and 2.6 kcal/mol, two elementary steps that refer to formation and decomposition of a tetrahedral intermediate are involved in the hydrolytic reaction, respectively. Polarized by Glu132, His272 serves as a proton carrier along the whole hydrolysis reaction. The electrostatic influence analysis highlighted residue Leu248 as a possible mutation target for rational design of LinB in enzyme modification. Further spatial location analysis provided explanation for the opposite effect of Asn38 toward the two elementary steps. Getting insight into the catalytic details as well as the structure and function of LinB can enrich the knowledge of it and promote its application in bioremediation of chlorinated hydrocarbon pollutants.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Dielectric and Antiferroelectric Properties of AgNbO3 Films Deposited on Different Electrodes

AgNbO3 antiferroelectric materials have become a hot topic due to their typical double polarization&ndash;electric field loops. AgNbO3 films usually exhibit superior properties to bulks. In this work, AgNbO3 films were fabricated via the pulsed laser deposition on (001) SrTiO3 substrate with (La0.5Sr0.5)CoO3, LaNiO3 and SrRuO3 bottom electrodes, in which the (La0.5Sr0.5)CoO3, LaNiO3 and SrRuO3 bottom electrodes were used to regulate the in-plane compressive stress of AgNbO3 films. It is found that AgNbO3 films deposited on (La0.5Sr0.5)CoO3, LaNiO3 and SrRuO3 bottom electrodes are epitaxial with dense microstructure. In changing the bottom electrodes from (La0.5Sr0.5)CoO3, LaNiO3 to SrRuO3, the in-plane compressive stress of AgNbO3 thin films becomes weaker, which leads to increased relative dielectric permittivity and reduced antiferroelectric&ndash;ferroelectric phase transition electric field EF from 272 kV/cm to 190 kV/cm. The reduced EF implies weakened antiferroelectric stability in AgNbO3 films. It can be seen that the antiferroelectric stability of AgNbO3 films could be regulated by changing the bottom electrodes

Applying optimized YOLOv8 for heritage conservation: enhanced object detection in Jiangnan traditional private gardens

Abstract This study aims to promote the protection and inheritance of cultural heritage in private gardens in the Jiangnan area of China. By establishing a precise visual labeling system and accelerating the construction of a database for private garden features, we deepen the understanding of garden design philosophy. To this end, we propose an improved Jiangnan private garden recognition model based on You Only Look Once (YOLO) v8. This model is particularly suitable for processing garden environments with characteristics such as single or complex structures, rich depth of field, and cluttered targets, effectively enhancing the accuracy and efficiency of object recognition. This design integrates the Diverse Branch Block (DBB), Bidirectional Feature Pyramid Network (BiFPN), and Dynamic Head modules (DyHead) to optimize model accuracy, feature fusion, and object detection representational capability, respectively. The enhancements elevated the model's accuracy by 8.7%, achieving a mean average precision ([email protected]) value of 57.1%. A specialized dataset, comprising 4890 images and encapsulating various angles and lighting conditions of Jiangnan private gardens, was constructed to realize this. Following manual annotation and the application of diverse data augmentation strategies, the dataset bolsters the generalization and robustness of the model. Experimental outcomes reveal that, compared to its predecessor, the improved model has witnessed increments of 15.16%, 3.25%, and 11.88% in precision, mAP0.5, and mAP0.5:0.95 metrics, respectively, demonstrating exemplary performance in the accuracy and real-time recognition of garden target elements. This research not only furnishes robust technical support for the digitization and intelligent research of Jiangnan private gardens but also provides a potent methodological reference for object detection and classification research in analogous domains

A kinetic study on mercury oxidation by HCl over typical Mn-based SCR catalysts

A kinetic approach was adopted to analyze the reaction mechanism of elemental mercury (Hg0) oxidation by HCl over three Mn-based low-temperature SCR catalysts, MnOx/TiO2, Fe-MnOx/TiO2 and CeMnO3. Experimental results validated that Hg0 oxidation efficiencies of the catalysts were promoted by HCl at different temperatures. The kinetic models for the heterogeneous Hg0 oxidation were established and verified based on the experimental data. The verification results demonstrated that Hg0 oxidation over the Mn-based catalysts follows Langmuir-Hinshelwood mechanism. The kinetic parameters of reaction rate constant and HCl adsorption constant were calculated from the model fitting equations. The reaction rate constant was raised with the increase of temperature, while the HCl adsorption constant presented the opposite trend. The Mn-based catalysts with the reaction rate constant of 84.17–335.77 s−1 showed the advantage over some other catalysts such as commercial V2O5-(WO3)/TiO2 and CeO2/TiO2. The kinetic parameters were further improved in the presence of O2. The apparent activation energy for Hg0 oxidation over the catalysts that was derived from the kinetic parameters was 4.13–12.53 kJ/mol, which was much lower than that of the other approaches. The advantageous kinetic parameters and apparent activation energy were favorable for the Mn-based catalysts to act as low-temperature SCR catalyst for synergistic Hg0 removal. The kinetic study results were of fundamental significance for designing the reactor according to the known flue gas conditions, used catalyst and required Hg0 oxidation efficiency.This research was supported financially by the National Natural Science Foundation of China (No. 52106169), the Natural Science Foundation of Shandong Province (No. ZR2021QE031), the China Postdoctoral Science Foundation (No. 2021M690097), the Foundation of State Key Laboratory of Coal Combustion (No. FSKLCCA2205), Taishan Scholars (No. ts201712003), the Postdoctoral Applied Research Project of Qingdao, and the Fundamental Research Funds of Shandong University (No. 2020GN008).Peer reviewe

Comparative transcription profiling of mRNA and lncRNA in pulmonary arterial hypertension after C75 treatment

Abstract Objectives To investigate mRNA and long non-coding RNA (lncRNA) expression profiles in monocrotaline (MCT)- mice. Materials and methods Lung tissues (Control-Vehicle, MCT-Vehicle, and MCT-C75) were examined by high-throughput sequencing (HTS). Aberrantly expressed mRNAs and lncRNAs were analyzed by bioinformatics. Cell proliferation and cell cycle analysis were performed to detect the potential protective effects of C75, an inhibitor of fatty acid synthase. The signaling pathways associated with inflammatory responses were verified by real time-PCR. Results RNA sequencing data reveals 285 differentially expressed genes (DEGs) and 147 lncRNAs in the MCT-Vehicle group compared to the control. After five-week of C75 treatment, 514 DEGs and 84 lncRNAs are aberrant compared to the MCT-Vehicle group. Analysis of DEGs and lncRNA target genes reveals that they were enriched in pathways related to cell cycle, cell division, and vascular smooth muscle contraction that contributes to the PAH pathological process. Subsequently, the expression of eight DEGs and three lncRNAs is verified using RT-PCR. Differentially expressed lncRNAs (ENSMUSG00000110393.2, Gm38850, ENSMUSG00000100465.1, ENSMUSG00000110399.1) may associate in PAH pathogenesis as suggested by co-expression network analysis. C75 can protect against MCT-induced PAH through its anti-inflammatory and anti-proliferation. Conclusions These DEGs and lncRNAs can be considered as novel candidate regulators of PAH pathogenesis. We propose that C75 treatment can partially reverse PAH pathogenesis through modulating cell cycle, cell proliferation, and anti-inflammatory

Influence of SO3 on the MnOx/TiO2 SCR catalyst for elemental mercury removal and the function of Fe modification

Elemental mercury (Hg0) is a highly hazardous pollutant of coal combustion. The low-temperature SCR catalyst of MnOx/TiO2 can efficiently remove Hg0 in coal-burning flue gas. Considering its sulfur sensitivity, the effect of SO3 on the catalytic efficiency of MnOx/TiO2 and Fe modified MnOx/TiO2 for Hg0 removal was investigated comprehensively for the first time. Characterizations of Hg-TPD and XPS were conducted to explore the catalytic mechanisms of Hg0 removal processes under different conditions. Hg0 removal efficiency of MnOx/TiO2 was inhibited irreversibly from 92% to approximately 60% with the addition of 50 ppm SO3 at 150 ℃, which resulted from the transformation of Mn4+ and chemisorbed oxygen to MnSO4. The existence of H2O would intensify the inhibitory effect. The inhibition almost disappeared and even converted to promotion as the temperature increased to 250 ℃ and above. Fe modification on MnOx/TiO2 improved the Hg0 removal performance in the presence of SO3. The addition of SO3 caused only a slight inhibition of 1.9% on Hg0 removal efficiency of Fe modified MnOx/TiO2 in simulated coal-fired flue gas, and the efficiency maintained good stability during a 12 h experimental period. This work would be conducive to the future application of MnOx/TiO2 for synergistic Hg0 removal.This research was supported financially by the National Natural Science Foundation of China (No. 52106169), the Natural Science Foundation of Shandong Province (No. ZR2021QE031), the China Postdoctoral Science Foundation (No. 2021M690097), the Foundation of State Key Laboratory of Coal Combustion (No. FSKLCCA2205), Taishan Scholars (No. ts201712003), the Postdoctoral Applied Research Project of Qingdao, and the Fundamental Research Funds of Shandong University (No. 2020GN008)Peer reviewe