59 research outputs found
A multifunctional tripodal fluorescent probe for the recognition of Cr3+, Al3+, Zn2+ and F− with controllable ESIPT processes
Three 4-(benzo[d]thiazol-2-yl)-2,5-dihydroxybenzaldehyde fluorophores were introduced to construct a tripodal multifunctional ESPIT fluorescence probe L. The fluorescent analysis revealed that probe L exhibited excellent recognition capabilities towards Cr3+, Al3+, Zn2+ and F− ions with large Stokes shifts. Furthermore, under optimal conditions, the detection limit of probe L towards Cr3+, Al3+, Zn2+ and F− were low, of the order of 10−8 M, which indicated that probe L was sensitive to these four ions. Interestingly, the fluorescent and 1H NMR titration experiments revealed that the recognition mechanism of probe L towards the ions Cr3+, Al3+, Zn2+ and F− were different. The presence of Cr3+ and Al3+ recovered the ESIPT, but the presence of Zn2+ trigger a moderate deprotonation of the phenolic OH and induced an ESIPT red-shifted (60 nm) emission wavelength. Finally, the presence of F− completely deprotonated the free phenolic OH and a remarkable red-shifted (130 nm) ESIPT emission was observed. In other words, the ESIPT process of probe L is controllable. Furthermore, the utility of probe L as a biosensor in living cells (PC3 cells) towards Cr3+, Al3+ and Zn2+ ions has been demonstrated
The Study of Mode Switching behavior of PSR J0614+2229 Using the Parkes Ultra-wideband Receiver Observations
In this paper, we presented a detailed single pulse and polarization study of
PSR J0614+2229 based on the archived data observed on 2019 August 15 (MJD
58710) and September 12 (MJD 58738) using the Ultra-wideband Low-frequency
Receiver on the Parkes radio telescope. The single-pulse sequences show that
this pulsar switches between two emission states, in which the emission of
state A occurs earlier than that of state B in pulse longitude. We found that
the variation in relative brightness between the two states is related to time
and both states follow a simple power law very well. Based on the phase-aligned
multi-frequency profiles, we found that there is a significant difference in
the distributions of spectral index across the emission regions of the two
states. Furthermore, we obtained the emission height evolution for the two
emission states and found that, at a fixed frequency, the emission height of
state A is higher than that of state B. What is even more interesting is that
the emission heights of both states A and B have not changed with frequency.
Our results suggest that the mode switching of this pulsar is possibly caused
by changes in the emission heights that alter the distributions of spectral
index across the emission regions of states A and B resulting in the
frequency-dependent behaviors, i.e., intensity and pulse width
Regulatory controls of duplicated gene expression during fiber development in allotetraploid cotton.
Polyploidy complicates transcriptional regulation and increases phenotypic diversity in organisms. The dynamics of genetic regulation of gene expression between coresident subgenomes in polyploids remains to be understood. Here we document the genetic regulation of fiber development in allotetraploid cotton Gossypium hirsutum by sequencing 376 genomes and 2,215 time-series transcriptomes. We characterize 1,258 genes comprising 36 genetic modules that control staged fiber development and uncover genetic components governing their partitioned expression relative to subgenomic duplicated genes (homoeologs). Only about 30% of fiber quality-related homoeologs show phenotypically favorable allele aggregation in cultivars, highlighting the potential for subgenome additivity in fiber improvement. We envision a genome-enabled breeding strategy, with particular attention to 48 favorable alleles related to fiber phenotypes that have been subjected to purifying selection during domestication. Our work delineates the dynamics of gene regulation during fiber development and highlights the potential of subgenomic coordination underpinning phenotypes in polyploid plants. [Abstract copyright: © 2023. The Author(s).
The NLRP3 inflammasome is involved in resident intruder paradigm-induced aggressive behaviors in mice
Background: Aggressive behaviors are one of the most important negative behaviors that seriously endangers human health. Also, the central para-inflammation of microglia triggered by stress can affect neurological function, plasticity, and behavior. NLRP3 integrates stress-related signals and is a key driver of this neural para-inflammation. However, it is unclear whether the NLRP3 inflammasome is implicated in the development of aggressive behaviors.Methods: First, aggressive behavior model mice were established using the resident intruder paradigm. Then, aggressive behaviors were determined with open-field tests (OFT), elevated plus-maze (EPM), and aggressive behavior tests (AT). Moreover, the expression of P2X7R and NLRP3 inflammasome complexes were assessed by immunofluorescence and Western blot. The levels of NLRP3 and inflammatory cytokines were evaluated using enzyme-linked immunosorbent assay (ELISA) kits. Finally, nerve plasticity damage was observed by immunofluorescence, transmission electron microscope, and BrdU staining.Results: Overall, the resident intruder paradigm induced aggressive behaviors, activated the hippocampal P2X7R and NLRP3 inflammasome, and promoted the release of proinflammatory cytokines IL-1β in mice. Moreover, NLRP3 knockdown, administration of P2X7R antagonist (A804598), and IL-1β blocker (IL-1Ra) prevented NLRP3 inflammasome-driven inflammatory responses and ameliorated resident intruder paradigm-induced aggressive behaviors. Also, the resident intruder paradigm promoted the activation of mouse microglia, damaging synapses in the hippocampus, and suppressing hippocampal regeneration in mice. Besides, NLRP3 knockdown, administration of A804598, and IL-1Ra inhibited the activation of microglia, improved synaptic damage, and restored hippocampal regeneration.Conclusion: The NLRP3 inflammasome-driven inflammatory response contributed to resident intruder paradigm-induced aggressive behavior, which might be related to neuroplasticity. Therefore, the NLRP3 inflammasome can be a potential target to treat aggressive behavior-related mental illnesses
Three-point Step Size Gradient Method with Relaxed Generalized Armijo Step Size Rule
Based on differences of points and differences of gradients over the most
recent three iterations, together with the Taylor's theorem, two forms of the
quasi-Newton equations at the recent iteration are constructed. By using the
two forms of the quasi-Newton equation and the method of least squares,
three-point step size gradient methods for solving unconstrained optimization
problem are proposed. It is proved by using the relaxed generalized Armijo step
size rule that the new method is of global convergence properties if the
gradient function is uniformly continuous. Moreover, it is shown that, when the
objective function is pseudo-convex (quasi-convex) function, the new method has
strong convergence results. In addition, it is also shown under some suitable
assumptions that the new method is of super-linear and linear convergence.
Although multi-piont information is used, TBB has the feature of simplicity,
low memory requirement and only first order information being used, the new
method is very suitable for solving large-scale optimization problems.
Numerical experiments are provided and the efficiency, robustness and analysis
of TBB are confirmed
A Framework of Abnormal Behavior Detection and Classification Based on Big Trajectory Data for Mobile Networks
Big trajectory data feature analysis for mobile networks is a popular big data analysis task. Due to the large coverage and complexity of the mobile networks, it is difficult to define and detect anomalies in urban motion behavior. Some existing methods are not suitable for the detection of abnormal urban vehicle trajectories because they use the limited single detection techniques, such as determining the common patterns. In this study, we propose a framework for urban trajectory modeling and anomaly detection. Our framework takes into account the fact that anomalous behavior manifests the overall shape of unusual locations and trajectories in the spatial domain as well as the way these locations appear. Therefore, this study determines the peripheral features required for anomaly detection, including spatial location, sequence, and behavioral features. Then, we explore sports behaviors from the three types of features and build a taxi trajectory model for anomaly detection. Anomaly detection, including sports behaviors, are (i) detour behavior detection using an algorithm for global router anomaly detection of trajectories having a pair of same starting and ending points; this method is based on the isolation forest algorithm; (ii) local speed anomaly detection based on the DBSCAN algorithm; and (iii) local shape anomaly detection based on the local outlier factor algorithm. Using a real-life dataset, we demonstrate the effectiveness of our methods in detecting outliers. Furthermore, experiments show that the proposed algorithms perform better than the classical algorithm in terms of high accuracy and recall rate; thus, the proposed methods can accurately detect drivers’ abnormal behavior
Comparative Phenotypic and Transcriptomic Analyses Provide Novel Insights into the Molecular Mechanism of Seed Germination in Response to Low Temperature Stress in Alfalfa
Low temperature is the most common abiotic factor that usually occurs during the seed germination of alfalfa (Medicago sativa L.). However, the potential regulatory mechanisms involved in alfalfa seed germination under low temperature stress are still ambiguous. Therefore, to determine the relevant key genes and pathways, the phenotypic and transcriptomic analyses of low-temperature sensitive (Instict) and low-temperature tolerant (Sardi10) alfalfa were conducted at 6 and 15 h of seed germination under normal (20 °C) and low (10 °C) temperature conditions. Germination phenotypic results showed that Sardi10 had the strongest germination ability under low temperatures, which was manifested by the higher germination-related indicators. Further transcriptome analysis indicated that differentially expressed genes were mainly enriched in galactose metabolism and carbon metabolism pathways, which were the most commonly enriched in two alfalfa genotypes. Additionally, fatty acid metabolism and glutathione metabolism pathways were preferably enriched in Sardi10 alfalfa. The Weighted Gene Co-Expression Network Analysis (WGCNA) suggested that genes were closely related to galactose metabolism, fatty acid metabolism, and glutathione metabolism in Sardi10 alfalfa at the module with the highest correlation (6 h of germination under low temperature). Finally, qRT-PCR analysis further validated the related genes involved in the above pathways, which might play crucial roles in regulating seed germination of alfalfa under low temperature conditions. These findings provide new insights into the molecular mechanisms of seed germination underlying the low temperature stress in alfalfa
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