Carrier dependent ferromagnetism in chromium doped topological insulator Cr0.2BixSb1.8−xTe3Cr_{0.2}Bi_xSb_{1.8-x}Te_3

Carrier-independent ferromagnetism of chromium doped topological insulator $Bi_xSb_{2-x}Te_3$ thin films,which cannot be explained by current theory of dilute magnetic semiconductor, has been reported recently. To study if it is related to the distinctive surface state of topological insulator, we studied the structural, magnetic and transport characters of $Cr_{0.2}Bi_xSb_{1.8-x}Te_3$ single crystals. The Curie temperature $T_c$, which is determined from magnetization and anomalous Hall effect measurements by Arrott plots, is found to be proportional to $p^{1/3}$, where p is the hole density. This fact supports a scenario of RKKY interaction with mean-field approximation. This carrier density dependent nature enables tuning and controlling of the magnetic properties by applying a gate voltage in the future science researches and spintronics applications.Comment: 6 pages, 3 figure

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Progress on modeling and simulation of directional solidification of superalloy turbine blade casting

Directional solidified turbine blades of Ni-based superalloy are widely used as key parts of the gas turbine engines. The mechanical properties of the blade are greatly influenced by the final microstructure and the grain orientation determined directly by the grain selector geometry of the casting. In this paper, mathematical models were proposed for three dimensional simulation of the grain growth and microstructure evolution in directional solidification of turbine blade casting. Ray-tracing method was applied to calculate the temperature variation of the blade. Based on the thermo model of heat transfer, the competitive grain growth within the starter block and the spiral of the grain selector, the grain growth in the blade and the microstructure evolution were simulated via a modified Cellular Automaton method. Validation experiments were carried out, and the measured results were compared quantitatively with the predicted results. The simulated cooling curves and microstructures corresponded well with the experimental results. The proposed models could be used to predict the grain morphology and the competitive grain evolution during directional solidification

Numerical simulation for the investment casting process of a large-size titanium alloy thin-wall casing

To optimize the investment casting process when producing high quality large-size titanium alloy thin-wall components is a time-consuming job due to the complicated metallurgical process. Numerical simulation is a high-efficiency method compared with trial and error, and therefore is introduced to the investment casting process optimization to shorten the new product development cycle and reduce the production cost. In this study, weakly compressible model (WCM) and ununiformed finite difference mesh (UFDM) was developed to reduce the memory consumption and ensure the simulation efficiency. The precision of the WCM and UFDM were verified by numerical simulation of cavity heat convection in a square cavity and hydraulics simulation of centrifugal filling in a transparent cavity. The numerical simulation of the investment casting process of a titanium alloy thin-wall casing under different process conditions was accomplished using a self-developed software, and the distribution characteristics of potential shrinkage defects were predicted. It was found that the predicted defects in the titanium alloy casing matched well with the actual X-ray experimental results. For the components investigated in this paper, more numerical simulation results show that the centrifugal casting process with respect to gravity casting had no obvious improvements in the concentrated shrinkage defects, and the gravity casting process can be more reasonable from the engineering point of view. Keywords: Titanium alloy, Investment casting, Numerical simulation, Finite difference method, Non-uniform mes

TMT-based quantitative proteomic analysis of spheroid cells of endometrial cancer possessing cancer stem cell properties

Abstract Background Cancer stem cells (CSCs) play an important role in endometrial cancer progression and it is potential to isolate CSCs from spheroid cells. Further understanding of spheroid cells at protein level would help find novel CSC markers. Methods Spheroid cells from endometrial cancer cell lines, Ishikawa and HEC1A, exhibited increased colony forming, subsphere forming, chemo-drug resistance, migration, invasion ability and tumorigenicity, verifying their cancer stem-like cell properties. The up-regulated CD90, CD117, CD133 and W5C5 expression also indicated stemness of spheroid cells. TMT-based quantitative proteomic analysis was performed to explore the potential alterations between parent cells and cancer stem-like spheroid cells. HK2-siRNA was transfected to Ishikawa and HEC1A cells to explore the roles and molecular mechanism of HK2 in endometrial cancer. Results We identified and quantified a total of 5735 proteins and 167 overlapped differentially expressed proteins of two cell types, 43 proteins were up-regulated and 124 were down-regulated in spheroid cells comparing with parent cells. KEGG pathway revealed a significant role of HIF-1 pathway in spheroid cells. qRT-PCR and western blot results of GPRC5A, PFKFB3 and HK2 of HIF-1 pathway confirmed their elevated expressions in spheroid cells which were consistent with proteomic results. HK2 promoted cancer stemness in endometrial cancer. Conclusion These findings indicate that spheroid cells from endometrial cancer cell lines possess cancer stem-like cell properties and enrich CSCs. HIF-1 pathway is activated in endometrial cancer stem-like spheroid cells

Microstructure, Mechanical Properties, and Constitutive Models for Ti–6Al–4V Alloy Fabricated by Selective Laser Melting (SLM)

The mechanical performances and microstructure of Ti–6Al–4V built by selective laser melting were evaluated by optical microscopy, transmission electron microscopy, and room temperature tensile testing, and compared with the wrought and as-cast material. The flow behavior of the as-produced Ti–6Al–4V at temperatures varying from 700–900 °C at an interval of 50 °C and strain rates ranging from 10−2–101 s−1 was experimentally acquired. According to the experimental measurement, the Johnson–Cook, modified Arrhenius model, and artificial neural network were constructed. A comparative investigation on the predictability of established models was performed. The as-produced microstructure is made up of non-equilibrium martensite and columnar grains, leading to higher strength and lower ductility with respect to the conventional material. In room temperature tensile tests, the SLMed Ti–6Al–4V shows the characteristics of continuous yielding and unobvious work-hardening. The flow stress rapidly reaches the peak, and the softening rate depends on the strain rates and deformed temperatures in hot compression. The Johnson–Cook model could well predict the flow stress during quasi-static tensile deformation, but the model constants might vary with the process conditions. For dynamic compression, the artificial neural network exhibits higher accuracy to fit the flow stress of SLMed Ti–6Al–4V, and higher error to predict the conditions out of the model data, compared to the modified Arrhenius model involving the compensation of strain rate and strain

Interfacial Synthesis of an Ultrathin Two-Dimensional Polymer Film via [2 + 2] Photocycloaddition

A carbon–carbon-linked, ultrathin, two-dimensional (2D) polymer film was prepared at the air/water interface through photochemically triggered [2 + 2] cycloaddition. The preorganization of the monomers on the water surface and the subsequent photo-polymerization led to the successful preparation of the ultrathin 2D polymer film. The obtained film is continuous, free standing, and has a large area (over 50 μm2). Transmission electron microscopy (TEM) and atomic force microscopy (AFM) give clear evidence of the ultrathin film morphology. Raman spectroscopy and X-ray photoelectron spectroscopy (XPS) indicate successful photo-induced [2 + 2] polymerization

A Data-Driven Approach to Identify Major Air Pollutants in Shanghai Port Area and Their Contributing Factors

Air pollution is a growing concern in metropolitan areas worldwide, and Shanghai, as one of the world’s busiest ports, faces significant challenges in local air pollution control. Assessing the contribution of a specific port to air pollution is essential for effective environmental management and public health improvement, making the analysis of air pollution contributions at a selected port in Shanghai a pertinent research focus. This study aims to delve into the distribution patterns of atmospheric pollutants in port areas and their influencing factors, utilizing a data-driven approach to unveil the relationship between pollution sources and dispersion. Through a comparative analysis of pollution levels in the port’s interior, surrounding regions, and urban area concentrations, we ascertain that carbon monoxide (CO) and nitric oxide (NO) are the primary pollutants in the port, with concentrations significantly exceeding those of the surrounding areas and urban area levels. These two pollutants exhibit an hourly pattern, with lower levels during the day and higher concentrations at night. Employing a random forest model, this study quantitatively analyzes the contribution rates of different factors to pollutant concentrations. The results indicate that NO concentration is primarily influenced by operational intensity and wind speed, while CO concentration is mainly affected by meteorological factors. Further, an orthogonal experiment reveals that maintaining daily operational vehicle numbers within 5000 effectively controls NO pollution, especially at low wind speeds. Additionally, humidity and temperature exhibit similar trends in influencing NO and CO, with heightened pollution occurring within the range of 75% to 90% humidity and 6 °C to 10 °C temperature. Severe pollution accumulates under stagnant wind conditions with wind speeds below 0.2 m/s. The results help to explore the underlying mechanisms of port pollution further and use machine learning for early pollution prediction, aiding timely warnings and emission reduction strategy formulation

Research progress of preservative coating of natural polymer materials

The planting area and output of fruits and vegetables in China are the first in the world. However, due to the imperfection of storage and transportation fresh-keeping technology, the annual loss of fruits and vegetables in China is serious. Rational use of fresh-keeping coating is of great significance for improving the shelf life of fruits and vegetables. Natural polymer preservative coating is widely concerned in the field of fruits and vegetables due to its wide sources, degradability, low price and good film-forming ability. In this paper, the types of natural polymer preservative coating were reviewed, and its application in the freshkeeping of fruits and vegetables was summarized and prospected, so as to provide Reference for further research