Optimizing nitrogen fertilizer and straw management promote root extension and nitrogen uptake to improve grain yield and nitrogen use efficiency of winter wheat (<i>Triticum aestivum L.</i>)

Straw returning is an efficient straw usage strategy in rice-wheat rotation, but nitrogen (N) use efficiency (NUE) was decreased due to incorrect straw and N fertilizer managements. To investigate the effects of straw and N fertilizer management on root growth, N fertilizer fates, grain yield and NUE of wheat, a two-year field and micro-plot 15N-labelled experiment under three levels of N application rate (0, 180 and 240 kg N ha−1) with two basal N application stages [seeding (BN), and 3-leaf stage (TN)] and three straw treatments [no straw return (NS), straw return by rotary tillage (SR) and straw return by ploughing (SP)] was conducted. The results indicated that SP increased grain yield and NUE, and the increase was highest under TN180. SP increased N uptake by enhancing root extension and soil N supply capacity, and TN decreased 15N residual in 60–100 cm soil layer. SP and TN180 both decreased 15N fertilizer loss and increased 15N recovery. Reducing basal N and applied at third-leaf stage (TN180) under SP had the same grain yield level as conventional N management (BN240) under NS, while highly improved NUE due to more root extension in deep soil layer and less N fertilizer loss.</p

DataSheet_1_Low red/far-red ratio can induce cytokinin degradation resulting in the inhibition of tillering in wheat (Triticum aestivum L.).docx

Shoot branching is inhibited by a low red/far-red ratio (R/FR). Prior studies have shown that the R/FR suppressed Arabidopsis thaliana branching by promotes bud abscisic acid (ABA) accumulation directly. Given that wheat tiller buds are wrapped in leaf sheaths and may not respond rapidly to a R/FR, systemic cytokinin (CTK) may be more critical. Here, systemic hormonal signals including indole-3-acetic acid (IAA), gibberellins (GA) and CTK and bud ABA signals in wheat were tested under a low R/FR. The results showed that a low R/FR reduced the percentage of tiller occurrence of tiller IV and the tiller number per plant. The low R/FR did not rapidly induced ABA accumulation in the tiller IV because of the protection of the leaf sheath and had little effect on IAA content and signaling in the tiller nodes. The significant change in the CTK levels was observed earlier than those of other hormone (ABA, IAA and GA) and exogenous cytokinin restored the CTK levels and tiller number per plant under low R/FR conditions. Further analysis revealed that the decrease in cytokinin levels was mainly associated with upregulation of cytokinin degradation genes (TaCKX5, TaCKX11) in tiller nodes. In addition, exposure to a decreased R/FR upregulated the expression of GA biosynthesis genes (TaGA20ox1, TaGA3ox2), resulting in elevated GA levels, which might further promote CTK degradation in tiller nodes and inhibit tillering. Therefore, our results provide evidence that the enhancement of cytokinin degradation is a novel mechanism underlying the wheat tillering response to a low R/FR.</p