MOESM1 of Loki zupa (Luooukezupa) decoction reduced airway inflammation in an OVA-induced asthma mouse model

Additional file 1. Ethical approval documen

MOESM2 of Loki zupa (Luooukezupa) decoction reduced airway inflammation in an OVA-induced asthma mouse model

Additional file 2. The ARRIVE Guidelines Checklist

Additional file 2: of Genotyping of polymorphic effectors of Toxoplasma gondii isolates from China

GRA3 translations alignment. Aligning the full length of GRA3 translations of those strains, shows that Xz7 is the only one which has a longer length compared with the others. GRA3 of Wh6 and Xz8 strains are homologous to that of type II. The amino acid sequence from 131 to 180 is the mutation cluster region constituting a major part of all mutations. (TIFF 4457 kb

Additional file 4: Table S4. of Variation detection based on next-generation sequencing of type Chinese 1 strains of Toxoplasma gondii with different virulence from China

All the chosen genes and variations in those gene. (XLSX 26 kb

The genotypes and geographic regions of <i>T. gondii</i> Chinese isolates.

<p>Panel A: a map of China to show the cumulative data of geographic distribution where the Chinese isolates of <i>T. gondii</i> used for genotyping were collected. The provinces and cities where <i>T. gondii</i> isolates were obtained and indicated by red dots and black dots, respectively. Red dots represent the locations (counties) where the isolates were collected in this work. Black dots indicate the locations (counties) where the isolates were collected in the early studies. SX: Shanxi province; SD: Shandong province; HN: Henan province; AH: Anhui province; JS: Jiangsu province; HuB: Hubei province; HuN: Hunan province; GD: Guangdong province; ZJ: Zhejiang province; FJ: Fujian province; YN: Yunnan province; QH: Qinghai province; GS: Gansu province; XJ: Xinjiang province; BJ: Beijing city; SH: Shanghai city. Panel B: a: the cumulative data of genotypes of <i>T. gondii</i> Chinese isolates. b: the genotypes of <i>T. gondii</i> isolates from human patients in mainland China. This is the first report of the genotype ToxoDB#204 from humans.</p

Multilocus PCR-RFLP genotyping of <i>T. gondii</i> from animals and humans in China.

a<p>μ−1 and μ−2 represent unique RFLP genotypes, respectively.</p

Summary of mouse virulence of representative strains with differents genotypes from China.

<p>Summary of mouse virulence of representative strains with differents genotypes from China.</p

The medical data from patients with toxoplasmosis.

a<p>F, female; M, male;</p>b<p>IC, immunocompromised; HCC, hepato-cellular carcinoma;</p

Phylogenetic network analysis of <i>Toxoplasma gondii</i> isolates from humans and animals in China.

<p>From the 23 samples, 5 genotypes were identified. Genotype number (ToxoDB PCR-RFLP #) and the representative strains are listed for each taxonomic branch. The representative strains from each genotype were underlined found in this study. The numbers in parentheses indicate the number of isolates from this study belonging to that genotype. The phylogenetic network analysis with EF1, HP2, UPRT1, UPRT7, GRA6 and GRA7 shows a perfect match with PCR-RFLP allele types at 10 multilocus markers used in the present study.</p

The kinetics of infection in mice with TgCtwh6 isolates.

<p>Kinetics of infection in blood and organs after oral infection with 50 cysts of TgCtwh6 isolate (genotype Chinese 1) in mice. I: the number of DNA copies in blood and tissues of the TgCtwh6 isolate post infection at various intervals by qPCR. Each point represents the mean value of the <i>T. gondii</i> DNA copies for five mice ± SD. II: detection of PCR products of <i>T. gondii</i> 529 bp fragments extracted from brain or ascitic fluid in the recipient mice. Abbreviations: <b>A</b>; blood, <b>B</b>; heart, <b>C</b>; liver, <b>D</b>; brain, and <b>E</b>; lymph node. b, n, and p represent blank, negative and positive control. *<i>P</i><0.05.</p