Additional file 1: Table S1. of Canonical correlation analysis (CCA) of anthropometric parameters and physical activities with blood lipids

Descriptive statistics for anthropometric parameters, physical activities and blood lipids from Hubei province in 2013, n = 5878. (DOCX 15 kb

Low FOXD3 expression was assotiated with poor prognosis in HGG patients.

<p>A and B: Kaplan-Meier curves OS and PFS in all HGG patients according to the expression levels of FOXD3, respectively. C and D: Kaplan-Meier curves OS and PFS in grade III glioma patients according to the expression levels of FOXD3, respectively. E and F: Kaplan-Meier curves OS and PFS in grade IV glioma patients according to the expression levels of FOXD3, respectively.</p

FOXD3 expression in HGG tissues and glioma cell lines.

<p>A: FOXD3 mRNA expression in 23 HGG cases and 13 control tissues. B: FOXD3 protein expression in 23 HGG cases and 13 control tissues. C: Representative protein expression of FOXD3 in 7 HGG cases and 5 control tissues by western blotting. D and E: Representative immunohistochemical staining of high expression and low FOXD3 expression in HGG cases, respectively. F and G: FOXD3 expression in five glioma cell lines by western blotting. Scale bar, 50μm.</p

Relationship between FOXD3 expression and clinicopathological features of 184 patients with HGGs.

<p>Abbreviations: GTR, gross total removal; KPS score, Karnofsky performance score; NTR, near total removal.</p><p>Relationship between FOXD3 expression and clinicopathological features of 184 patients with HGGs.</p

FOXD3 overexpression suppressed proliferation and enhanced starvation-induced apoptosis in U87MG cells.

<p>A: Western blotting analysis of FOXD3 and cleaved caspase-3 expression in U87MG cells. B: Cell proliferation assay showing significantly enhanced proliferation rate of FOXD3-overexpressed U87MG cells in comparison with EV-treated U87MG cells (<i>P</i><0.05) by MTT assay. C: Representative images of EdU assay in U87MG cells. D: Statistical results from five independent EdU assays. E: Representative results of apoptosis assay by flow cytometry in serum-starvated U87MG cells. F: Statistical results from three independent apoptosis assays. Summarized results are expressed as mean ±SD. EV, empy vector; NC, negative control. Scale bar, 50μm.</p

FOXD3 silencing promoted proliferation and inhibited starvation-induced apoptosis in SW1080 cells.

<p>A: Western blotting analysis of FOXD3 and cleaved caspase-3 expression in SW1080 cells. B: Cell proliferation assay showing significantly enhanced proliferation rate of FOXD3-silenced SW1080 cells in comparison with siNC-treated SW1080 cells (<i>P</i><0.05) by MTT assay. C: Representative images of EdU assay in SW1080 cells. D: Statistical results from five independent EdU assays. E: Representative results of apoptosis assay by flow cytometry in serum-starvated SW1080 cells. F: Statistical results from three independent apoptosis assays. Summarized results are expressed as mean ±SD. EV, empy vector; NC, negative control. Scale bar, 50μm.</p